SUNHua-li,SONGJian-kun,LIDing-li,et al.StudyonthePhysiologicalDynamicChangeofDifferentGraftCombinationsin PearDuringtheGraftedHealingProcess[J].Northern Horticulture,2013,37(16):25-29.
梨不同嫁接组合嫁接愈合过程中生理动态变化研究
- Title:
- StudyonthePhysiologicalDynamicChangeofDifferentGraftCombinationsin PearDuringtheGraftedHealingProcess
- 文章编号:
- 1001-0009(2013)16-0025-05
- Keywords:
- pear; graftcompatibility; healingprocess; physiologicaldynamicchange
- 分类号:
- S661.2
- 文献标志码:
- A
- 摘要:
- 以亲和性不同的2种嫁接组合‘鸭梨/豆梨’、‘OHF51/豆梨’为试材，研究了嫁接口部位在嫁接愈合过程中其多酚氧化酶（PPO）活性、苯丙氨酸解氨酶（PAL）活性、酚类物质含量、可溶性糖含量、可溶性蛋白含量、木质素含量等指标的变化情况，以期探究梨嫁接亲和的机理。结果表明：2个嫁接组合的PPO、PAL活性变化趋势基本一致，嫁接亲和性较好的‘鸭梨/豆梨’组合的PPO和PAL活性最高峰值均高于亲和性较差的‘OHF51/豆梨’组合；2个组合的多酚含量相近，且变化趋势基本相同，在愈合过程中总体呈下降趋势；‘鸭梨/豆梨’组合的可溶性糖含量在愈合过程前期高于‘OHF51/豆梨’组合，但后期下降较快；2个组合的可溶性蛋白含量的变化趋势基本一致，总体呈现先升高后降低的过程，嫁接愈合后期基本恢复到初始水平；‘鸭梨/豆梨’组合木质素含量在嫁接愈合初期是逐渐升高的，后期下降后上升。而‘OHF51/豆梨’嫁接组合在嫁接前期木质素含量一直呈下降趋势，后期木质素含量逐渐升高。
- Abstract:
- Takingtwodifferentgraftcombinationsof‘Yali/ P.calleryana Dcne’and‘OHF51/P.calleryanaDcne’asmaterials，thephysiologicaldynamicchangeincludingpolyphenoloxidase(PPO)andphenylalnineammonialyase(PAL)activity，polyphenol，solublesugar，solubleproteinandlignincontentduringgrafthealingprocesswerestudied，inordertoexplorethemechanismofgraftcompatibility.TheresultsshowedthatthechangetrendofactivityofPPOandPALwerebasicallythesame.ThehighestpeakvalueofPPOactivityandPALin‘Yali/P.calleryanaDcne’combinationwithagoodaffinitywerehigherthanthatin‘OHF51/P.cvalleryanaDcne’combinationwithapooraffinity；thepolyphenolcontentintwocombinationsshoweddecliningtrendonthewholewithasimilarchangecurveandtherewasnosensibledifferenceincontentbetweentwocombinations；thesolublesugarcontentin‘Yali/P.calleryana cne’combinationwashigherthanthatin‘OHF51/P.calleryanaDcne’combinationduringmostoftheperiod，butdeclinedrapidlyatthelatestage；thechangetrendofsolubleproteincontentintwocombinationswasbasicallythesameandshowedtheprocessoffirstincreasedandthendecreased，atthelatestageofhealingprocess，recoveredtothenormallevel；thelignincontentin‘Yali/P.calleryanaDcne’combinationwasgraduallyincreasedatearlystageofgrafthealingprocess，whilein‘OHF51/P.calleryanaDcne’combination，thelignincontenthadbeendecliningatfirststage，thengraduallyincreasedatlatestage.
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备注/Memo
第一作者简介：孙华丽（1986-），女，硕士研究生，研究方向为梨树砧木育种。
责任作者：王然（1960-），女，博士，教授，研究方向为梨树育种。E-mail:qauwr@126.com.
基金项目：国家现代梨产业技术体系资助项目（nycytx-29-06）。
收稿日期：2013-04-08