LI Li,JIANG Tao,ZHOU An-pei,et al.Diversity Analysis of Gene Expression at Dormancy Stage of Lateral Buds in Populus yunnanensis by cDNA-AFLP Markers[J].Northern Horticulture,2013,37(15):117-121.
滇杨侧芽休眠期基因表达多样性的cDNA-AFLP分析
- Title:
- Diversity Analysis of Gene Expression at Dormancy Stage of Lateral Buds in Populus yunnanensis by cDNA-AFLP Markers
- 文章编号:
- 1001-0009(2013)15-0117-05
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- 为探讨滇杨分枝特性差异的分子机理，对采集于四川省和云南省的59个滇杨优树无性系3 a生苗木进行了cDNAAFLP差异研究，分析了苗木侧芽休眠时基因表达的多样性。结果表明：筛选出的7对引物组合共扩增出了总条带608条，其中有353条差异性条带，Nei’s基因多样性指数为0.1061，Shannon信息指数为0.1856，遗传分化系数为0.0123，居群间的基因流为40.28，表明居群间的遗传分化程度较低。分子生物学方差分析（AMOVA）结果表明，居群间的差异仅为0.99%，差异不显著（P=0.052），居群内差异占99.01%，差异极显著；表明滇杨侧芽在休眠期个体之间基因表达的复杂性；聚类分析结果显示，在亚分枝单元或最小分枝单元，大部分无性系能够以分枝性状相聚。该研究结果为进一步开展滇杨分枝相关基因的筛选及克隆等工作奠定了前期研究基础。
- Abstract:
- To reveal the molecular mechanism of branching characteristic difference in Populus yunnanensis,taking 59 three-year-old seedlings of plus trees clones of P.yunnanensis collected from Sichuan Province and Yunnan Province as the tested materials,cDNA AFLP molecular technique was conducted to examine the diversity of gene expression at dormancy stage of lateral buds of the seedlings.The results showed that a total of 608 bands including 353 different bands were amplified using selected seven primer combinations.Nei’s gene diversity index and Shannon’s information index were 0.1061 and 0.1856 respectively.Gene differentiation coefficience was 0.0167,and gene flow was 29.42,which indicated a lower genetic differentiation existed among populations of P.yunnanensis.Analysis of molecular variance (AMOVA) showed that variance was 0.99% among populations and 99.01% within population.Moreover,the variance only was highly and significantly different among population.The results suggested that gene expression was complicated at dormancy stage of lateral buds.Furthermore,the clustering results showed that most of the clones held the same branching characteristics fell into subcluster unit or single cluster unit.The results of this study laid the foundation of the previous research for further screening and cloning of genes related to branching in P.yunnanensis.
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备注/Memo
第一作者简介：李里（1985-），男，云南德宏人，硕士研究生，现主要从事林木遗传育种等研究工作。责任作者：何承忠（1970-），男，甘肃民勤人，博士，教授，现主要从事林木遗传育种与分子生物学方面的研究工作。E-mail：hcz70@163.com.
基金项目：国家自然科学基金资助项目（30960320）；国家林业公益性行业专项基金资助项目（201104076）；云南省中青年学术与技术带头人后备人才培养基金资助项目（2012HB021）。
收稿日期：2013-04-08