SHEN Shun-xian,LI Xue-hui,HE Ai-li,et al.Study on Micropropagation Technique System of Mentha spicata Linn[J].Northern Horticulture,2013,37(13):146-149.
留兰香微繁技术体系研究
- Title:
- Study on Micropropagation Technique System of Mentha spicata Linn
- 文章编号:
- 1001-0009(2013)13-0146-04
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- 以留兰香春季萌发的含顶芽幼嫩茎段为外植体，利用正交实验设计等方法研究了留兰香微繁技术体系。结果表明：用70%～75%酒精浸洗30 s，0.1%升汞浸洗6 min对外植体消毒灭菌效果最好，适宜芽诱导的培养基是MS+NAA 0.05 mg/L+6BA 1.5 mg/L+30 g/L蔗糖+7 g/L琼脂，最佳芽增殖培养基是MS＋6BA 0.5 mg/L＋NAA 0.01 mg/L＋GA3 0.1 mg/L＋20～30 g/L蔗糖＋7 g/L琼脂，最佳生根培养基是1/2MS＋IBA 0.2 mg/L＋KT 0～0.05 mg/L＋20～25 g/L蔗糖＋7 g/L琼脂。
- Abstract:
- Using the tender stem containing terminal bud in spring as explants，the orthogonal test was used,the micropropagation technique system of Mentha spicata ?Linn were studied.The results showed that the best method for disinfectant was 70% to 75% alcohol (washed for 30 s) and 01% mercuric chloride (washed for 6 min).The optimal medium for inducing shoot was MS+NAA 0.05 mg/L+6BA 1.5 mg/L+30 g/L sugar+7 g/L agar；the optimal medium for shoot generation was MS+6BA 0.5 mg/L+NAA 0.01 mg/L+GA3?0.1 mg/L+20 g/L to 30 g/L sugar+7 g/L agar；the optimal medium for rooting was 1/2MS+IBA 0.2 mg/L+KT 0 to 0.05 mg/L+20 g/L to 25 g/L sugar+7 g/L agar.
参考文献/References:
[1]沙红,廖康.药用植物留兰香的组织培养研究[J].新疆农业大学学报,2003,26(1):10-12.
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备注/Memo
第一作者简介：申顺先（1978-），男，本科，讲师，现主要从事作物遗传育种与植物组培快繁技术教学与科研工作。E-mail:shenshunxian@163.com.