WANG Xiao-xi,GAO Zeng-gui,ZHANG Shuo,et al.Study on Identification of Tomato Biotyoe Fusarium oxysporum f.sp.lycopersici[J].Northern Horticulture,2013,37(11):100-103.
尖孢镰孢菌番茄专化型的鉴定研究
- Title:
- Study on Identification of Tomato Biotyoe Fusarium oxysporum f.sp.lycopersici
- 文章编号:
- 1001-0009(2013)11-0100-04
- Keywords:
- usarium oxysporum; tomato fusarium wilt; rDNA-ITS; cross inoculation
- 分类号:
- S 436.412.1+9
- 文献标志码:
- A
- 摘要:
- 以27株镰孢菌菌株为试材，采用菌株形态学观察、rDNA-ITS序列分析以及寄主专化型鉴定相结合的方法,鉴定了尖孢镰孢菌番茄专化型。结果表明：经形态学和rDNA-ITS测序筛选，最后筛选出10株尖孢镰孢菌。将尖孢镰孢菌交叉接种到不同寄主植物，确定菌株编号YH070001和DL070009的镰孢菌为尖孢镰孢菌番茄专化型。与单一的传统方法相比，ITS rDNA方法有着较高的灵敏度，所以采取形态学、现代分子生物学以及传统交叉接种相结合的鉴定方式，能够使菌株专化型的鉴定更加准确、完善。
- Abstract:
- Taking 27 Fusarium strains as materials,F.oxysporum f.sp.lycopersici was identified using morphology observation and rDNA-ITS sequencing.The results showed that after selected by morphology and rDNA-ITS sequencing,10 F.oxysporum strains were identified by traditional method of specialized form as cross inoculation.Thus N0.YH070001 and DL070009 were confirmed F.oxysporum f.sp.lycopersici. Compared with single traditional method,rDNA-ITS sequencing has higher sensitivity.To test the accuracy and feasibility of this identification method,morphology observation,rDNA-ITS sequencing and traditional method of specialized form identification were used in this study.
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备注/Memo
第一作者简介：王小皙（1986-），男，硕士,研究方向为蔬菜病害。E-mail:wangxiaoxi1986@163.com.责任作者：高增贵（1966-），男，内蒙古准格尔旗人，博士，研究员，博士生导师，研究方向为玉米病害和蔬菜病害生物防治。E-mail:gaozenggui@sina.com.
基金项目：辽宁省科技攻关资助项目（2011214002）；沈阳市科技攻关专项资助项目（F12-119-3-00）。收稿日期：2013-01-17