WU Ya-ping,TANG Dao-cheng.Conservation in vitro and Establishment of the Propagation System on Male Sterile Lines of Marigold[J].Northern Horticulture,2013,37(01):116-118.
万寿菊雄性不育系离体保存及快繁体系的建立
- Title:
- Conservation in vitro and Establishment of the Propagation System on Male Sterile Lines of Marigold
- 文章编号:
- 1001-0009（2013）01-0116-03
- 分类号:
- S 681.903.6
- 文献标志码:
- A
- 摘要:
- 以9个万寿菊雄性不育系植株的腋芽为外植体,进行诱导、继代和生根培养基配方的优化研究。结果表明：适合万寿菊雄性不育系腋芽诱导丛生芽的培养基最佳配方是：MS+6-BA 0.3 mg/L+NAA 0.10 mg/L；适合丛生芽继代增殖培养的培养基最佳配方是：MS+6-BA 0.5 mg/L+NAA 0.10 mg/L；适合生根的培养基最佳配方是：1/2MS+IBA 0.10 mg/L。初步建成了万寿菊雄性不育系离体保存体系。
- Abstract:
- Lateral buds in nine male sterile lines of marigold were used as explants to screen their culture medium of induction，subculture and rooting in vitro.The results showed that the culture medium was suitable for lateral bud in male sterile lines of marigold in inducing cluster buds was MS+6-BA 0.3 mg/L+NAA 0.10 mg/L;The culture medium was suitable for subculture of cluster buds was MS+6-BA 0.5 mg/L+NAA 0.10 mg/L；the best culture medium for rooting was 1/2MS+IBA 0.10 mg/L.So far the concervation system in vitro of male sterile lines on marigold were preliminarily established.
参考文献/References:
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备注/Memo
第一作者简介：伍亚平（1986-），女，在读硕士，现主要从事植物遗传育种工作。E-mail:wuyaping812@163.com.