LI Wei,YU Ze-yuan,LI Xing-guo,et al.Comparison of Fruit Nutritional Traits and Isoenzymes in Different Ploidy Striffin Melon[J].Northern Horticulture,2013,37(01):95-98.
不同倍性薄皮甜瓜果实几种成分及同工酶比较研究
- Title:
- Comparison of Fruit Nutritional Traits and Isoenzymes in Different Ploidy Striffin Melon
- 文章编号:
- 1001-0009（2013）01-0095-04
- Keywords:
- striffin melon; autopolyploidy; isoenzymes; fruit nutritional traits
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以二倍体薄皮甜瓜品种及其人工诱导的同源四倍体、三倍体为试材，对不同倍性薄皮甜瓜果实营养品质及叶片EST、POD、SOD同工酶谱带进行研究。结果表明：不同倍性薄皮甜瓜果实成熟时期可溶性糖、可溶性固形物、可溶性蛋白质、维生素C、氨基酸含量存在差异，可溶性糖、维生素C、氨基酸含量有同样趋势，为四倍体＞三倍体＞二倍体；中心可溶性固形物、可溶性蛋白质含量有同样趋势，为三倍体＞四倍体＞二倍体；叶片中，二倍体和三倍体EST谱带均比同源四倍体多2条；而POD和SOD在谱带条数上没有差异，只是多倍体谱带亮度较二倍体强，表明其表达量较高，说明它们的酶种类相似但剂量不同。
- Abstract:
- Taking different ploidy striffin melon materials，including diploid，triploid crossed with 4××2× and autotetraploid induced by colchicine were selected for text their nutritional traits and the variabilities of EST，POD and SOD isoenzymes in leaves.The results showed that the content of soluble sugar，content of soluble solids,content of soluble protein，content of VC and content of amino acid were divergent in different ploidy striffin melon as follow：soluble sugar，VC and amino acid contents made the same trend，4×>3×>2×；soluble solids and soluble protein contents made the same trend，3×>4×>2×；the EST isoenzyme bands of diploid and triploid were all two more than those of autotetraploid in leaves.The number of POD and SOD isoenzyme bands of different ploidy melon materials were same，but the expressed quantity of polyploid was higher.The results indicated the isoenzyme band pattern of different ploidy melon materials were same,but the activities in polyploid were stronger than that in the diploid.
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备注/Memo
第一作者简介：李为（1984-），男，硕士，研究方向为果树生理学。E-mail：liwei85541446@163.com.