[1]刘威,蓝祖栽,叶云峰,等.药水苏白绢病病原鉴定及其生防木霉菌株筛选[J].北方园艺,2013,37(05):125-128.
　LIU Wei,LAN Zu-zai,YE Yun-feng,et al.Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain[J].Northern Horticulture,2013,37(05):125-128.
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药水苏白绢病病原鉴定及其生防木霉菌株筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年05期 页码: 125-128 栏目: 出版日期: 2013-03-15
Title:
Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain
文章编号:
1001-0009(2013)05-0125-04
作者:
刘威; 蓝祖栽; 叶云峰; 蒋妮; 刘丽辉
广西壮族自治区药用植物园，广西药用资源保护与遗传改良重点实验室，广西 南宁 530023
Author(s):
LIU Wei; LAN Zu-zai; YE Yun-feng; JIANG Ni; LIU Li-hui
Guangxi Botanical Garden of Medicinal Plant，Guangxi Medicinal Resources Conservation and Genetic Improvement Laboratory，Nanning，Guangxi 530023
关键词:
药水苏; 白绢病; 病原鉴定; 拮抗木霉; 筛选
Keywords:
Betonica officinalis; Sclerotium rolfsii; identification of the pathogen; antagonistic trichoderma strain; screen
分类号:
S 567.23+9
文献标志码:
A
摘要:
以药水苏白绢病株为试材，通过致病性测定法确定病原菌，采用病原菌的形态鉴定方法并结合该菌的ITS rDNA序列分析鉴定病原菌种类；采用平皿对峙法筛选拮抗菌株。结果表明：药水苏白绢病的病原菌为罗氏白绢小菌核菌（Sclerotium rolfsii Sacc.）；5个供试木霉菌株中以哈茨木霉（H2）对病原菌的抑制作用最强，拮抗指数为Ⅱ级，抑菌率为86.27%。
Abstract:
Taking diseased plants of Betonica officinalis as experimental materials，the pathogen was identified by pathogenicity test；the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis；the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc；among the five tested trichoderma strains，Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis，its antagonistic index and control efficiency were Ⅱ level and 86.27%，respectively.

参考文献/References:

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备注/Memo

第一作者简介：刘威（1982-），女，广西北流人，本科，助理工程师，现主要从事药用植物病虫害等研究工作。
基金项目：广西省卫生厅自筹基金资助项目（Z2011161）。
收稿日期：2012-11-09

更新日期/Last Update: 2014-08-22