REN Xi-bo,DAI Xi-yao,ZHANG Jun-hua,et al.Study on Differences of The Amylase Activity in Different Raphanus sativus L.[J].Northern Horticulture,2012,36(22):21-23.
不同萝卜品种淀粉酶活性的差异研究
- Title:
- Study on Differences of The Amylase Activity in Different Raphanus sativus L.
- 文章编号:
- 1001-0009(2012)22-0021-03
- Keywords:
- Raphanus sativus L.(radish）; amylase activity; variety
- 分类号:
- S 631.1
- 文献标志码:
- A
- 摘要:
- 以25个萝卜品系和组合为试材，采用田间随机区组设计，肉质根采收后采用3,5-二硝基水杨酸法测定萝卜α,β-淀粉酶活性及总酶活性，探讨不同萝卜α,β-淀粉酶活性及总酶活性的差异并建立α,β-淀粉酶及总淀粉酶活性之间的关系。结果表明：萝卜不同品种和组合的α，β-淀粉酶活性及总淀粉酶酶活性淀粉酶活性差异显著，其中A04、S01、A17、A20、S05和A07的α-淀粉酶活性较高；β-淀粉酶活性及总淀粉酶酶活性较高的品系同为A19、A06、A17、A12和S03。α-淀粉酶活性占总酶活性的比例为8.11%～25.57%，β-淀粉酶活性为75.26%～91.89%，三者之间关系为Y总淀粉酶活性=-0.029+1.039Xα-淀粉酶活性+0.998Xβ-淀粉酶活性。
- Abstract:
- To determine the α,β-amylase activity and total amylase activity in 25 radish varieties，field experiments used randomized block design and repeated 3 times，α-amylase，β-amylase and total amylase activity were estimated by the 3，5-dinitrosalicylic acid method after fleshy root harvest，for providing a reference and establishment relationship of α-amylase，β-amylase and total amylase activity.The results showed that there were significance differences of α-amylase，β-amylase and total amylase activity in different radish varieties；the A04，S01，A17，A20，S05 and A07 were the higher α-amylase activity and the A19，A06，A17，A12 and S03 were the the higher β-amylase and total amylase activity.The ratio of α，β-amylase activity in total amylase activity was 8.11% to 25.57% and 75.26%~91.89%，respectively and the relationship of them was YTotal amylase activity=-0.029+1.039Xα-amylase activity+0.998Xβ-amylase activity.
参考文献/References:
[1]
李合生
.
植物生理生化实验原理和技术
[M].
北京
:
高等教育出版社
,2000:169-172.
[2]罗志刚,杨景峰,罗发兴.α-淀粉酶的性质及应用[J].食品研究与开发,2007,128(8):163-164. [3]卫秀英,薛香,李一卓.硝基水杨酸法与凝胶扩散法测定小麦α-淀粉酶活性的比较[J].种子,2007,26(10):68-70. [4]岳海凤.小麦α-淀粉酶活性与主要品质性状的相关性及其遗传分析[D].保定：河北农业大学,2009:5-6. [5]岳海凤,郜庆炉,薛香.小麦α-淀粉酶活性测定方法比较[J].陕西农业科学,2008(6):6-7.
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备注/Memo
第一作者简介：任喜波（1978-），女，博士，副教授，研究方向为蔬菜遗传育种。E-mail:renxibo@163.com.