XIAO Zhong-jiu,LI Xiao-xia,XU Ai-bin,et al.Biological Characteristic of Leaf Blight:A New Cycad Disease in Guizhou Province[J].Northern Horticulture,2012,36(16):117-120.
贵州省铁树新病害叶枯病病原菌生物学特性研究
- Title:
- Biological Characteristic of Leaf Blight:A New Cycad Disease in Guizhou Province
- 文章编号:
- 1001-0009(2012)16-0117-04
- Keywords:
- cycad; Phomopsis cycadis; biological characteristic
- 分类号:
- S 436.8(273)
- 文献标志码:
- A
- 摘要:
- 对贵州省铁树上发生的一种新病害-叶枯病病原菌进行了生物学特性研究,探讨不同温度、pH、光照、碳源及氮源等条件对该病原体菌丝生长、分生孢子萌发的影响,以期系统掌握该病原菌的生物学特性,为铁树叶枯病防治提供理论依据。结果表明:铁树叶枯病菌菌丝在10~35℃温度范围内均能生长,在15~35℃之间孢子均能萌发,菌丝生长和孢子萌发最适温度分别为30℃和25℃;在pH 2~12之间,病原菌菌丝(孢子)均能生长(萌发),其中pH为6时病原菌菌丝生长最好,pH为10时病原菌孢子萌发率最高;光照条件对菌丝的扩展和孢子萌发影响不明显;该病原菌对单糖、双糖、多糖等碳源以及有机氮和无机氮均能利用,其中以蔗糖作碳源时,菌丝生长和孢子萌发最佳,以硝酸钾作氮源时,菌丝生长最好,供试氮源对分生孢子的萌发均不佳。
- Abstract:
- This paper aimed to study the biological characteristics of the pathogen (Phomopsis cycadis)causing leaf blight on cycad in Guizhou province,the effects of temperature,pH,illumination,the sources of nitrogen and carbon on the mycelium growth and the germination of conidiospore of the pathogen were explored,the study of this paper systematically made a research on the biological characteristics of Phomopsis cycadis,it could provide a theoretical basis for the prevention and control of the cycad disease.The temperature range was 10~35℃ for the mycelia growth and 15~35℃ for the conidiospore germination of the pathogen,the optimal temperature for mycelia growth and conidiospore germination were 30℃ and 25℃.The pathogen could grow and conidiospore germination with a pH of 2~12,while the optimal pH was 6.0 for mycelia growth and 10 for conidiospore germination.Light hardly affected the growth of fungus mycelium and the conidiospore germination.The pathogen could use monosaccharide,disaccharide,polysaccharide,the best mycelia growth and conidiospore germination were observed with sucrose as the carbon source,and the pathogen could use organic and inorganic nitrogen,the best mycelia growth were observed with KNO3 as the nitrogen source,but not suitable for conidiospore germination.
参考文献/References:
[1]Kowalskarnt,Itzhak y,Puett D.Presence of aromatase inhibitors in cycads[J].JEthnopharmacol,1995,47(3):113-116. [2]江苏新医学院.中药大词典[M].上海:上海科学技术出版社,1979:491-49. [3]孔繁翠,顾昊,王鹤尧.铁树提取物对人肺腺癌细胞凋亡的诱导及相关机制的初步研究 [J].中国新药杂志,2008,17(8):667-672. [4]肖龙骞,朱华.苏铁nrDNAITS区的序列多态性:不完全致同进化的证据[J].生物多样性,2009,17(5):476-481. [5]Baker C A,Adkins S.Tobacco ringspot virus Found in the Cardboard Cycad (Zamia furfuracea) in Florida[J].Plant Disease,2007,91(1):112. [6]王新荣,李楠,林月娥.苏铁镰刀菌球茎腐烂病化学防治技术研究[J].中国森林病虫,2005,24(6):34-37. [7]方中达.植病研究方法[M].北京:中国农业出版社,1998:123-125,152-153. [8]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001:55-56. [9]董国菊,申晚霞.重庆地区玉米圆斑病菌生物学特性的测定[J].西南大学学报(自然科学版),2010,32(12):8-13. [10]李小霞,肖仲久,李 黛.草乌白绢病菌生物学特性研究[J].广东农业科学,2010(8):128-130. [11]卢东升,王明好,贾晓.野菊内生真菌生物多样性与生态分布[J].东北林业大学学报,2011,39(8):88-89. [12]刘超,师俊玲,周小娟,等.产PDG杜仲内生菌的分离筛选和分类鉴定及生长条件研究[J].西北农林科技大学学报(自然科学版),2011,39(1):203-209. [13]王玉美,陈洪章.产喜树碱内生真菌的筛选及鉴定[J].微生物学通报2011,38(6):884-888. [14]姜淑霞,马洪兵.板栗上的拟茎点霉一新种[J].菌物学报,2010,29(4):467-471. [15]郑华英,钱国良,徐福元,等.苏北杨树新造林地溃疡病病原菌的鉴定[J].林业科学研究,2010,23(5):649-655.
备注/Memo
第一作者简介:肖仲久(1980-),男,湖南武冈人,博士,副教授,现主要从事植物病原真菌学研究工作。E-mail:xzj198099@163.com.