MAO Hong-yu,WANG Ying,LIU Di,et al.Study on Rapid Micropropagation of Tulip Via in Tissue Culture[J].Northern Horticulture,2012,36(14):114-118.
郁金香离体快繁技术研究
- Title:
- Study on Rapid Micropropagation of Tulip Via in Tissue Culture
- 文章编号:
- 1001-0009（2012）14-0114-05
- Keywords:
- Tulipa; bulb scale; tissue culture; rapid micropropagation; hormone
- 分类号:
- S 682.2+63
- 文献标志码:
- A
- 摘要:
- 以2个郁金香品种“阿波罗”和“琳?马克”为试材，用不同时期、不同部位、不同类型的郁金香鳞片作为外植体，以MS为基本培养基，分别添加不同质量浓度的6-BA、NAA、IAA和活性炭，研究不同外植体以及不同培养基对小鳞茎诱导的影响。结果表明：经春化处理后的鳞片作为外植体诱导小鳞茎的效果最好，鳞片部位以中层最适宜诱导小鳞茎；以带鳞茎盘的双鳞片作诱导小鳞茎的外植体效果最好；最适宜“阿波罗”小鳞茎诱导的激素组合为 MS+6-BA 2 mg/L+ NAA 2 mg/L+IAA 0.3 mg/L；最适宜“琳?马克”小鳞茎诱导的激素组合为 MS+6-BA 2 mg/L+ NAA 2 mg/L+IAA 0.1 mg/L;活性炭对小鳞茎的诱导有促进作用；最适宜的暗处理时间为 7 d。
- Abstract:
- Chosen two ‘Apeldoorn’and ‘Leen van der Mark’cultivars as test materials,different period,different position and different type of bulbs as explants,MS as basal medium,added the different concentration 6-BA,NAA,IAA and activated carbon respectively,the effects of different explants and media on bulblets induction of tulip scapes as experimental material were studied.The results showed that the vernalized scale was the best appropriate explant.Twin-scale with basal plate was the best appropriate explant and the cultivation effect of middle layer scale was the best.The most suitable medium for bulblets induction of‘Apeldoorn’was MS+6-BA 2 mg/L+NAA 2 mg/L+IAA 0.3 mg/L.The most suitable medium for bulblets induction of‘Leen van der Mark’was MS+6-BA 2 mg/L+NAA 2 mg/L+IAA 0.1 mg/L.Activated carbon had favourable effect on bulblets induction.The most appropriate date of darkness was 7 days.
参考文献/References:
[1]梅莉娟.中国郁金香属植物物候特征及居群形态分化[D].乌鲁木齐:新疆农业大学,2006.
[2]王斐,琚淑明.郁金香组织培养的研究进展[J].安徽农业科学,2010,38(16):8332-8333.
[3]邓涛.郁金香新品种引种区试及种球繁育研究[D].杨凌:西北农林科技大学,2008.
[4]Wright N A,Alderson P G.The growth of tulip tissues in vitro [J].Acta Horticulturae,1980,109:263-270.
[5]Taeb A G,Alderson P G.Micropropagation of tulip optimizing shoot production from floral stem explants[J].Acta Horticulturae,1987,212:677-681.
[6]李艳,方宏筠.郁金香叶片直接诱导试管人工种球的快速繁殖的研究[J].辽宁师范大学学报,1998,21(2):144-147.
[7]尹新新,杨瑞卿,金建邦,等.郁金香组织培养技术[J].江苏农业科学,2010(5)：85-86.
[8]龚明霞,何铁光,方锋学,等.郁金香鳞片离体培养再生小鳞茎研究[J].广西农业科学,2010,41(11):1158-1160. [9]陆文梁,王雪洁,郭仲琛.郁金香的组织培养及器官分化的研究[J].园艺学报,1986,13(1):55-60.
[10]黄勇,张千朋,郭善利.郁金香快速繁殖的研究[J].聊城师院学报,1996,9(3):65-68.
[11]杨永刚,代汉萍,胡新颖,等.郁金香器官离体培养再生小鳞茎的研究[J].园艺学报,2006,33(5)：1133-1136. [12]胡新颖,王锦霞,代汉萍,等.郁金香鳞片组织培养研究[J].沈阳农业大学学报,2007,38(3):304-307.
[13]孙晓梅,孙琪,杨宏光,等.洋水仙Arkle离体快速繁殖体系的建立[J].西北农林科技大学学报(自然科学版),2010,38(3):173-178.
[14]孙晓梅,李妍,杨宏光,等.风信子离体快繁体系的建立[J].沈阳农业大学学报,2010,41(1):33-36.
[15]Capuana M,Giannlni R,Lambardi M.Micropropagation of muture plants of Cyperus [J].Acta Hortical,1991,289:91.
[16]Bach A.Effect of AC on in vitro propagation of Iris [J].Biological Science,1988,36(4/6):107.
[17]Mohamed-Yaseen Y.Micropropagation of Psidium L.by using explant of seedling [J].HortSci,1992,27(6):692.
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备注/Memo
第一作者简介：毛洪玉(1974-)，女，博士，副教授，现主要从事园林植物栽培生理研究工作。E-mail:maohongyu74@163.com.
收稿日期：2012-04-09