NIU Ru-xuan,ZHANG Jian-xia,WANG Yue-jin,et al.Research on the Embryo Rescue of Stenospemocarpic Seedless Grape[J].Northern Horticulture,2012,36(07):1-5.
种子败育型无核葡萄胚挽救研究
- Title:
- Research on the Embryo Rescue of Stenospemocarpic Seedless Grape
- 文章编号:
- 1001-0009(2012)07-0001-05
- Keywords:
- stenospemocarpic; seedless grapes; embryo rescue; sampling time; medium; screening
- 分类号:
- S 663.103.6
- 文献标志码:
- A
- 摘要:
- 为提高种子败育型无核葡萄胚挽救中胚的发育率和成苗率，以无核品种“红无籽露”、“波尔莱特”、“皇家秋天”为试材，研究了其在自然授粉条件下胚挽救的最佳取样时期，并对适宜胚培养的培养基进行了筛选。结果表明：“红无籽露”、“波尔莱特”、“皇家秋天”的最佳取样时期〖JP4〗分别为花后51～53、55和66 d；筛选获得的适宜培养基为MM4+0.5 mg/L GA3+1.5 mg/L IAA+〖JP3〗500 mg/L水解酪蛋白+60.0 g/L蔗糖+1.5 g/L活性炭+10.0 μmol/L ZnSO4+1.0 mmol/L 甘氨酸+1.0 mmol/L 半胱氨酸+100.0 mmol/L 甘露醇。“红无籽露”、“波尔莱特”、“皇家秋天”的胚发育率和成苗率分别为：15.00%和6.67%、24.00%和10.00%、10.00%和5.00%，平均达到16.33%和7.22%。
- Abstract:
- To improve the embryo development and germination in embryo rescue,taking stenospemocarpic seedless grapes of ‘Sultanina Rose’，‘Perlette’,‘Autumn Royal’ as test materials，the best timing for embryo rescue of the three species under natural pollination conditions were investigated,and the suitable media were selected for the cultivation of embryo culture.The results showed that the best sampling time for ‘Sultanina Rose’，‘Perlette’，‘Autumn Royal’were 51～53 d，55 d and 66 d after full bloom respectively.The optimum media that selected was MM4+0.5 mg/L GA3+1.5 mg/L IAA+500 mg/L hydrolyzed casein+60.0 g/L sucrose+1.5 g/L activated carbon+10.0 μmol/L ZnSO4+1.0 mmol/L glycine+1.0 mmol/L cysteine+100.0 mmol/L mannitol.The development rate and germination rate of ‘Sultanina Rose’，‘Perlette’and ‘Autumn Royal’s’ovules was 15.00% and 6.67%，24.00% and 10.00%，10.00% and 5.00% respectively，and an average of 16.33% and 7.22%.
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备注/Memo
第一作者简介：牛茹萱（1987-），女，甘肃兰州人，在读硕士，研究方向为葡萄种质资源与生物技术育种。E-mail：niuruxuan2006@163.com。
责任作者：张剑侠（1964-），男，博士，副教授，硕士生导师，现主要从事葡萄种质资源与生物技术育种研究工作。E-mail：zhangjx666@126.com。
基金项目：国家现代农业产业技术体系建设专项基金资助项目（CARS-30-yz-7）；公益性行业（农业）科研专项资助项目（200903044）；陕西省科技攻关计划资助项目（2011K02-10）。
收稿日期：2012-01-29