HAN Hong-yan,JI Ai-qing,LV Chen-chen,et al.The Study of Several Dates Varieties Lectin in Leaf for Blood Coagulation Effect[J].Northern Horticulture,2012,36(01):31-34.
相同生长条件下几种枣叶凝集素生物活性分析
- Title:
- The Study of Several Dates Varieties Lectin in Leaf for Blood Coagulation Effect
- 文章编号:
- 1001-0009(2012)01-0031-04
- Keywords:
- Date leaves; Lectin; blood cells; sugar suppression
- 分类号:
- S 665.1
- 文献标志码:
- A
- 摘要:
- 提取13个枣品种叶片中的凝集素,经硫酸铵沉淀、透析得到蛋白质提取液，用不同类型的红细胞检测凝集活性(人的A、B、AB、O型血)，并通过糖抑制试验比较凝集素的生物活性。结果表明：不同品种枣叶中凝集素活性各不相同；同种凝集素对不同种类的血细胞的凝集作用不同，如在凝集效价为20时，梨枣的枣叶凝集素对A、B、O、AB型血细胞分别为中度凝集、高度凝集、中度凝集、轻度凝集；不同凝集素对同一种血细胞的凝集作用也不同：如在凝集效价为20时，“壶瓶1号”对B型血细胞为高度凝集，“骏枣”对B型血细胞为中度凝集，“壶瓶6号”对B型血细胞为轻度凝集；糖对凝集素的凝集作用均有抑制，且抑制作用的强弱与其浓度和分子结构有关，葡萄糖和果糖的抑制作用较弱，而麦芽糖和蔗糖的抑制作用则较强。总体来看，“骏枣”和“壶瓶3号”的凝集作用最强，“壶瓶4号”的凝集作用最弱。
- Abstract:
- 13 dates Lectin of leaves extracted by ammonium sulfate precipitation, dialysis, got protein extracted fluid, which impacted different types of red blood cells detection agglutinate activity (people type A, type B, type AB, type O blood), and through the sugar suppression test, compared the biological activity of Lectin. The results showed that Lectin in different varieties dates had different activity; Same Lectin to different kinds of blood cells had different effect; As for the aggregation titer 20, date leaf Lectin of ‘Li Dates’ to type A, type B, type O, AB blood cells separately for moderate aggregation, highly agglutinate, moderate agglutinate, mild aggregation; Different kind of Lectin to same kind of blood cells had different aggregation function: As for the aggregation titer 20, ‘HuPing Date 1’ to type B cells for height of aggregation , ‘Jun Dates’ to type B cells for moderate aggregation, ‘HuPing 6’ to type B cells for mild aggregation; Sugar to the aggregation function of Lectin were restrained, and inhibiting effect was concerned with its concentration and molecular structure, the inhibition of glucose and fructose were relatively weak, but the inhibition of maltose and sucrose was stronger. In a word, ‘Jun Dates’ and ‘HuPing 6’ had the strongest aggregation function, HuPing 4 had the weakest aggregation function.
参考文献/References:
[1]Sharon N, Lis H. Lectins[A]. London:Chapman and Hall,1989:l-2.
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备注/Memo
第一作者简介：韩红艳（1976-），女，硕士，讲师，研究方向为园艺植物与细胞生物学。E-mail：hhy0822@126.com。