TIAN Yongheng,YANG Xinxin,ZHANG Xiangyue,et al.Effects of High-temperature Sealed Greenhouse on Soil Microbial Community Structure for Facility-grown Melon and Watermelon[J].Northern Horticulture,2026,(13):121-131.[doi:10.11937/bfyy.20254340]
高温闷棚对设施西甜瓜土壤微生物群落结构的影响
- Title:
- Effects of High-temperature Sealed Greenhouse on Soil Microbial Community Structure for Facility-grown Melon and Watermelon
- 文章编号:
- 1001-0009(2026)13-0121-11
- Keywords:
- high-temperature sealed greenhouse; root-knot nematode; soil microorganism; community structure
- 分类号:
- S436.5
- 文献标志码:
- A
- 摘要:
- 以高温闷棚前、闷棚7、14、21 d等时间点采集的土壤样品为试材，采用漏斗分离法、土壤微生物总DNA提取及高通量测序技术，研究了高温闷棚对设施西甜瓜土壤微生物群落及病害防治的影响，以期为高温闷棚技术的科学应用提供参考依据。结果表明:高温闷棚7 d左右对大棚0~30 cm土层根结线虫的灭杀效果达到100%；高温闷棚后，土壤细菌的丰富度、均匀度均降低，土壤真菌的丰富度降低、均匀度升高；高温闷棚对大棚土壤细菌群落的影响大于对真菌群落的影响；随着闷棚时间延长，土壤中镰刀菌属真菌的相对丰度呈减少趋势。高温闷棚可减少设施西甜瓜土壤中根结线虫、镰刀菌属真菌等有害微生物种群数量，有助于防治设施西甜瓜病害。
- Abstract:
- Taking soil samples collected at time points such as before high-temperature sealing,7 days,14 days,and 21 days of sealing as test materials,through the use of funnel separation method,total DNA extraction of soil microorganisms and high-throughput sequencing technology,the effects of high-temperature sealing on the soil microbial community and disease control of greenhouse sweet and melon crops were studied,in order to provide reference for the scientific application of high-temperature sealing technology.The results showed that high-temperature closed greenhouse treatment for approximately 7 days achieved 100% control efficacy against root-knot nematodes in the 0 to 30 cm soil layer of greenhouses.After treatment,both the richness and evenness of soil bacteria decreased,while the richness of soil fungi decreased and the evenness increased.The effect of high-temperature sealed greenhouse on the soil bacterial community was greater than that on the fungal community.With the prolongation of treatment time,the relative abundance of Fusarium fungi in soil showed a decreasing trend.High-temperature sealed greenhouse treatment can reduce the population of harmful microorganisms such as root-knot nematodes and Fusarium fungi in the soil of facility-grown melon and watermelon,thus helping to control related diseases.
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备注/Memo
第一作者简介：田永恒（1997-），男，硕士研究生，研究方向为病虫害绿色防控。E-mail：2695524318@qq.com.责任作者：张自启（1974-），男，本科，副研究员，现主要从事西甜瓜栽培和病虫害防治等研究工作。E-mail：lynkyzzq@126.com.基金项目：河南省西甜瓜产业技术体系洛阳综合试验站资助项目（HARS-22-10-Z1）。收稿日期：2025-12-18