ZHOU Yu,NIE Yulu,LIU Bo,et al.Domestication Cultivation and Antioxidant Activity of Seven Wild Ganoderma sichuanense Strains[J].Northern Horticulture,2026,(13):94-102.[doi:10.11937/bfyy.20252810]
七株野生赤芝驯化栽培及抗氧化活性
- Title:
- Domestication Cultivation and Antioxidant Activity of Seven Wild Ganoderma sichuanense Strains
- 文章编号:
- 1001-0009(2026)13-0094-09
- Keywords:
- Ganoderma sichuanense; domestication cultivation; bioactive compounds; antioxidant activity; germplasm resources
- 分类号:
- S567.31
- 文献标志码:
- A
- 摘要:
- 以贵州省黔西南州采集的7株野生赤芝（Ganoderma sichuanense）及栽培菌株“沪农1号”（HNLZ1）为试材，采用ITS序列分析进行分子鉴定，并利用代料栽培技术对其农艺性状、营养成分、活性物质含量及抗氧化活性进行系统研究，综合评价该野生资源的驯化潜力与育种价值，旨在为赤芝种质资源开发与品种选育提供参考依据。结果表明：所有野生菌株均鉴定为赤芝（自展值99%）；农艺性状分析显示菌株间存在显著差异，其中JZLZ5的子实体产量和生物学效率最优且孢子粉产量突出，JZLZ7的菌盖形态最佳；营养成分测定发现菌株纤维素含量呈梯度分化，JZLZ4含量最高，JZLZ2呈现“高蛋白-低脂肪”特性；活性物质分析表明菌株JZLZ1粗多糖、JZLZ3三萜类、JZLZ7麦角甾醇含量显著优于其他菌株及文献报道值；抗氧化活性评价揭示菌株JZLZ1（DPPH清除率）、JZLZ2（ABTS清除率）、JZLZ4/JZLZ5（羟自由基清除率）分别表现最优。
- Abstract:
- Taking seven wild strains of Ganoderma sichuanense collected from Qianxinan prefecture,Guizhou Province,and the cultivated strain ‘Hunong No.1’ (HNLZ1) as test materials,molecular identification was performed by ITS sequence analysis,and the agronomic traits,nutritional components,active substance contents,and antioxidant activities of the strains were systematically studied using the substitute cultivation technology,to comprehensively evaluate the domestication potential and breeding value of this wild resource，in order to provide references for the development of germplasm resources and variety breeding of Ganoderma lucidum.The results showed that all wild strains were identified as G.sichuanense (bootstrap value 99%).Analysis of agronomic traits revealed significant differences among strains.JZLZ5 exhibited the best sporocarp yield and biological efficiency,along with outstanding spore powder production,while JZLZ7 showed the optimal pileus morphology.Determination of nutritional components indicated a gradient divergence in cellulose content among strains,with JZLZ4 containing the highest level,and JZLZ2 displaying a ‘high-protein and low-fat’ characteristic.Analysis of active substances demonstrated that the contents of crude polysaccharides in JZLZ1,triterpenoids in JZLZ3,and ergosterol in JZLZ7 were significantly superior to those of other strains and the values reported in the literature.Evaluation of antioxidant activities revealed that JZLZ1 (DPPH scavenging rate),JZLZ2 (ABTS scavenging rate),and JZLZ4/JZLZ5 (hydroxyl radical scavenging rate) performed the best,respectively.
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备注/Memo
第一作者简介：周宇（1997-），男，硕士，农艺师，现主要从事食用菌栽培与育种等研究工作。E-mail：1964707578@qq.com.责任作者：龙声卫（1976-），男，本科，研究员，现主要从事现代山区特色农业关键技术与成果示范等研究工作。E-mail：1lsw3891619@163.com.基金项目：黔西南州农业林业科学研究院院级资助项目（2024-07）；黔西南州基础研究计划资助项目（州科合基础2024-9）；贵州省农业科技创新联盟科技协同创新资助项目(［2025］04)。收稿日期：2025-08-02