LIN Huifeng,XU Kezheng,LI Lihong,et al.Authenticity Identification of Spathiphyllum kochii F1 Hybrids Using SSR Markers and Morphology[J].Northern Horticulture,2026,(6):18-27.[doi:10.11937/bfyy.20252797]
基于SSR标记与形态学的白掌F1代杂种真实性鉴定
- Title:
- Authenticity Identification of Spathiphyllum kochii F1 Hybrids Using SSR Markers and Morphology
- 文章编号:
- 1001-0009(2026)06-0018-10
- 分类号:
- S682
- 文献标志码:
- A
- 摘要:
- 以白掌的9个亲本、8个杂种F1代以及“星光”ד美酒”的39个杂种F1代群体植株为试材，采用多态性SSR标记分析与形态学观测相结合的方法，研究了SSR标记与形态学方法在白掌F1代杂种真实性鉴定中的效率差异，建立高效、准确的早期鉴定技术体系，以期为白掌育种提供参考依据。结果表明：从16对多态性SSR引物中筛选出8对具有父本特异性或双亲互补型条带的引物，可用于杂种鉴定。8个杂交后代的SSR扩增均显示父本或双亲基因型，鉴定真实率为100%；形态学观测仅能明确鉴定其中6个后代（“青美”“星辉”“维美”“阳萌1号”“阳萌2号”和“维墨”）为真杂种，“玉手”与“萌香”难以从形态区分，形态学鉴定效率为75%。SSR标记鉴定效率显著高于形态学方法，尤其适用于未表现明显父本性状的真杂种。进一步利用P14标记对“星光”ד美酒”组合的39个后代检测，均扩增出父本带型，真实率为100%，再次验证了SSR标记的高效性与可靠性。综上，SSR标记鉴定准确、高效，可作为白掌杂交后代早期真实性鉴定的关键技术。
- Abstract:
- Taking nine parental lines of Spathiphyllum kochii，eight hybrid F1 progenies，and a population of 39 hybrid F1 plants from the cross ‘Xingguang’בMeijiu’ as the test materials，polymorphic SSR marker analysis with morphological observation were combined to compare the efficiency of SSR markers and morphological methods in authenticating the hybridity of Spathiphyllum kochii F1 progenies,in order to establish an efficient and accurate early identification system to support Spathiphyllum kochii breeding.The results showed that eight pairs of SSR primers with paternal specific or complementary parental bands were selected from 16 polymorphic primers for hybrid identification.SSR amplification of the eight hybrid progenies all displayed paternal or biparental genotypes，achieving an authentication rate of 100%.Morphological observation clearly identified only six of the progenies (‘Qingmei’‘Xinghui’ ‘Weimei’‘Yangmeng 1’‘Yangmeng 2’ and ‘Weimo’) as true hybrids，while ‘Yushou’ and ‘Mengxiang’ could not be reliably distinguished based on morphology，giving a morphological identification efficiency of 75%.The efficiency of SSR markers was significantly higher than that of morphological methods，especially for true hybrids that did not exhibit obvious paternal traits.Further detection using the P14 marker on the 39 progenies from the ‘Xingguang’בMeijiu’ cross all produced paternal band patterns，with a true hybrid rate of 100%，reconfirming the high efficiency and reliability of SSR markers.In conclusion，SSR marker identification is accurate and efficient，and can serve as a key technique for early authenticity assessment of Anthurium hybrid progenies.
参考文献/References:
［1］周辉明,林辉锋,莫智龙,等.四倍体白掌新品种‘绿萌’的选育［J］.热带作物学报,2022,43(5):964-970.［2］许克正,周辉明,甘玮欣,等.抑菌剂对白掌‘维美’组培苗生长的影响［J］.亚热带农业研究,2024,20(3):187-193.［3］CHEN J,MCCONNELL D B,HENNY R J，et al.The foliage plant industry［J］.Horticultural Reviews,2005,31:45-110.［4］周辉明,林辉锋,曹奕鸯,等.基于数量性状与SSR标记的白掌种质遗传关系分析［J］.福建农林大学学报(自然科学版),2024,53(3):314-322.［5］贾凯旋,张笑玲,孟义江,等.紫苏杂种F1的SSR标记鉴定及性状分析［J］.分子植物育种,2024,22(17):5701-5706.［6］陈梦洁,吕长平,秧拯民,等.基于SSR标记的牡丹杂交子代真实性鉴定［J］.分子植物育种,2023,21(6):1948-1954.［7］苏鸣.基于形态标记和分子标记的杜鹃花品种杂交子代真实性研究［D］.宁波:宁波大学,2021.［8］贾美鑫.芍药属组间远缘杂交后代优株筛选及杂种真实性鉴定［D］.泰安:山东农业大学,2023.［9］曹煜,李心彤,刘鹏,等.地被菊‘延农舒绾’ב延农朱颜’杂交后代形态学及耐盐性分析［J］.北方园艺,2025(15):98-107.［10］曹奕鸯,夏朝水,陈玮婷,等.应用SSR分子标记鉴定非洲菊F1代杂种的真实性［J］.福建农业学报,2023,38(7):842-850.［11］胡文舜,黄爱萍,姜帆,等.龙眼正反交后代的SSR鉴定及遗传多样性分析［J］.园艺学报,2015,42(10):1899-1908.［12］齐兰,黄丽云,杨玉娟,等.应用SSR和InDel标记鉴定槟榔杂交子代的真实性［J］.分子植物育种,2025,23(8):2663-2668.［13］郑崇文,王婉尧,白斌,等.利用微卫星标记鉴定核桃杂交子代的亲本［J］.四川林业科技,2024,45(2):103-108.［14］刘小飞,孙映波,黄丽丽,等.白掌SSR引物筛选及12份种质资源亲缘关系分析［J］.分子植物育种,2022,20(20):6802-6810.［15］周辉明,林辉锋,曹奕鸯,等.基于白掌株型突变转录组差异的SSR位点分析［J］.分子植物育种,2023,21(16):5365-5377.［16］李文秀,贺军军,张华林,等.SSR分子标记鉴定橡胶树F1真伪杂种［J］.热带作物学报,2021,42(5):1305-1309.［17］刘金梅,刘小飞,于波,等.不同白掌组合授粉结实和种子特性研究［J］.热带作物学报,2015,36(7):1213-1219.［18］OZAKI T,SHIMADA T,NAKANISHI T,et al.RAPD analysis for parentage determination in Prunus mume sieb.et zucc［J］.Engei Gakkai Zasshi,1995,64(2):235-242.［19］叶刚,张笑笑,陈静波,等.结缕草属植物杂交后代形态特征和SRAP分子标记鉴定［J］.草地学报,2021,29(7):1406-1415.［20］郝芳敏,马二磊,丁伟红,等.利用SSR分子标记鉴定甜瓜杂交种种子纯度［J］.北方园艺,2025(11):8-16.［21］文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较［J］.中国油料作物学报,2006,28(3):233-239.［22］尹跃,赵建华,何昕孺,等.枸杞种间杂交F1群体的SSR鉴定及遗传分析［J］.西北农业学报,2019,28(12):2027-2034.［23］黄郑晖,林焕章,曾巧英,等.甘蔗与斑茅杂交后代的SSR标记鉴定方法［J］.分子植物育种,2023,21(21):7085-7096.［24］周文才,田仟仟,李田,等.浙江红花油茶×广宁红花油茶杂交子代的表型性状及其SSR分子鉴定［J］.广西植物,2024,44(7):1269-1277.［25］田婉莹.基于SSR和InDel标记的海南荔枝种质资源遗传多样性分析及杂交后代鉴定研究［D］.海口:海南大学,2015.［26］POWELL W,MACHRAY G C,PROVAN J.Polymorphism revealed by simple sequence repeats［J］.Trends in Plant Science,1996,1(7):215-222.［27］GIANFRANCESCHI L,SEGLIAS N,TARCHINI R,et al.Simple sequence repeats for the genetic analysis of apple［J］.Theoretical and Applied Genetics,1998,96(8):1069-1076.［28］周宁宁,李淑斌,李远波,等.二倍体月季F1群体的SSR鉴定与遗传分析［J］.园艺学报,2017,44(1):151-160.［29］王业云.牡丹杂交后代的形态学和SSR分子标记鉴定［D］.北京:中国林业科学研究院,2016.［30］朱骏驰,郭印山,刘镇东,等.利用SSR分子标记鉴定葡萄F1代杂种［J］.沈阳农业大学学报,2016,47(2):148-152.
相似文献/References:
[1]李庆玲,陈荣,杨跃生,等.两种天南星科植物根尖组织染色体观察方法的优化[J].北方园艺,2012,36(02):134.
LI Qing-ling,CHEN Rong,YANG Yue-sheng,et al.Optimization of Chromosome Observing Method of Root-tip Tissue in Two Species of Araceae[J].Northern Horticulture,2012,36(6):134.
[2]赵红艳.引种地芦荟雄性不育的生殖生物学研究[J].北方园艺,2014,38(23):133.
ZHAO Hong-yan.Research on Reproductive Biology of Aloe Male Sterility in Introduction Area[J].Northern Horticulture,2014,38(6):133.
[3]王洁菲,曹支敏,白露超,等.陕甘两省花椒疫霉病病原鉴定及其拮抗菌筛选[J].北方园艺,2015,39(05):113.[doi:10.11937/bfyy.201505034]
WANG Jie-fei,CAO Zhi-min,BAI Lu-chao,et al.Identification of Phytophthora Species Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes[J].Northern Horticulture,2015,39(6):113.[doi:10.11937/bfyy.201505034]
[4]陈星星,邵秀丽,何松林.LED光源不同光质比对白掌组培苗生长的影响[J].北方园艺,2015,39(06):86.[doi:10.11937/bfyy.201506024]
CHEN Xing-xing,SHAO Xiu-li,HE Song-lin.Effect of Different Light Quality Ratios of LED on Growth of Spathiphyllum in vitro[J].Northern Horticulture,2015,39(6):86.[doi:10.11937/bfyy.201506024]
[5]毛 红 俊,孔 祥 生,张 妙 霞,等.香水白掌离体培养褐化反应的初步研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010066]
MAO Hong -jun,KONG Xiang -sheng,ZHANG Miao -xia,et al.The Preliminary Studies on Browning Reactions invitro of Spathyllum patinii[J].Northern Horticulture,2010,34(6):0.[doi:10.11937/bfyy.2010010066]
[6]宋冰,付永平,郭昱秀,等.一株野生侧耳属菌株的鉴定及生物学特性[J].北方园艺,2017,41(24):182.[doi:10.11937/bfyy.20171661]
SONG Bing,FU Yongping,GUO Yuxiu,et al.Identification and Biological Characteristics of a Wild Pleurotus sp．[J].Northern Horticulture,2017,41(6):182.[doi:10.11937/bfyy.20171661]
[7]马银鹏,焦枥禾,包旭翔,等.一种黑木耳菌包中病原菌的分离鉴定及其生物学特性[J].北方园艺,2023,(24):126.[doi:10.11937/bfyy.20231361]
MA Yinpeng,JIAO Lihe,BAO Xuxiang,et al.Isolation，Identification and Biological Characteristics of Pathogen in Auricularia heimuer Bag[J].Northern Horticulture,2023,(6):126.[doi:10.11937/bfyy.20231361]
[8]高晓芬,施艳萍,张黎.外源激素对蝴蝶兰光合生理与花芽分化的影响[J].北方园艺,2024,(13):57.[doi:10.11937/bfyy.20234381]
GAO Xiaofen,SHI Yanping,ZHANG Li.Effects of Exogenous Hormones on Photosynthetic Physiology and Flower Bud Differentiation in Phalaenopsis[J].Northern Horticulture,2024,(6):57.[doi:10.11937/bfyy.20234381]
[9]孙修远,高文凯,徐超,等.紫荆枝枯病及叶斑病病原菌种类鉴定[J].北方园艺,2025,(13):121.[doi:10.11937/bfyy.20250294]
SUN Xiuyuan,GAO Wenkai,XU Chao,et al.Identification of Cercis chinensis Shoot Canker and Leaf Spot Pathogen Species[J].Northern Horticulture,2025,(6):121.[doi:10.11937/bfyy.20250294]
备注/Memo
第一作者简介：林辉锋（1984-），男，硕士，副研究员，现主要从事花卉生物技术等研究工作。E-mail：289687622@qq.com.责任作者：周辉明（1980-），男，硕士，研究员，现主要从事花卉选育与生物技术等研究工作。E-mail:120914051@qq.com.基金项目：三明市科技计划资助项目(2024-N-2)；福建省农业引导性(重点)资助项目(2022N0037)。收稿日期：2025-07-31