WEI Shuhua,WANG Ling,ZHANG Yue,et al.Determination of Low-temperature Half-lethal Temperature and Evaluation of Cold Resistance of Fifteen Brassica rapa L.Varieties[J].Northern Horticulture,2026,(6):83-90.[doi:10.11937/bfyy.20252764]
十五份芜菁品种低温半致死温度的测定与抗寒性评价
- Title:
- Determination of Low-temperature Half-lethal Temperature and Evaluation of Cold Resistance of Fifteen Brassica rapa L.Varieties
- 文章编号:
- 1001-0009(2026)06-0083-08
- Keywords:
- Brassica rapa L.; low-temperature stress; semi-lethal temperature (LT50); low-temperature tolerance coefficient
- 分类号:
- S631.3
- 文献标志码:
- A
- 摘要:
- 以课题组收集到的15份地方品种芜菁种质资源为试材，采用人工模拟低温法，研究了四叶一心期芜菁幼苗在4、0、-2、-4、-6、-8 ℃低温处理下叶片相对电导率及生长与生理指标的响应，将各温度处理下的相对电导率以Logistic方程拟合以求低温半致死温度（LT50），结合主成分分析（PCA）与隶属函数法对15份材料的耐寒潜力进行了综合评价，以期为芜菁的抗寒育种以及在高寒地区的种植提供参考依据。结果表明：15份材料的低温半致死温度分别为-6.88、-5.95、-5.37、-5.16、-4.64、-4.21、-3.93、-2.56、-2.48、-2.38、-2.25、-1.98、-1.13、-1.09、-0.92 ℃，其中WJ1的低温半致死温度最低，WJ12的低温半致死温度最高；通过综合评价将15份材料分为2个耐寒型、9个中度耐寒型和4个寒冷敏感型，其中筛选出2个耐寒型材料WJ1和WJ6；得到了初步预测芜菁苗期抗寒能力的量化回归方程Y=-0.489+0.081X1+0.178X2+0.181X3。
- Abstract:
- Taking 15 local turnip (Brassica rapa L.) germplasm resources collected by the research team as experimental materials,artificial low-temperature simulation was conducted to investigate the responses of relative electrical conductivity (REC) of leaves,as well as growth and physiological indexes of turnip seedlings at four-leaf-and-one-heart stage under low temperature treatments of 4 ℃,0 ℃,-2 ℃,-4 ℃,-6 ℃ and -8 ℃.The REC data under each temperature treatment were fitted by Logistic equation to calculate the low-temperature semi-lethal temperature (LT50).Combined with principal component analysis (PCA) and membership function analysis to comprehensively evaluate the cold tolerance potential of the 15 materials,in order to provide reference for cold-resistant breeding of turnips and their cultivation in high-altitude cold regions.The results showed that the low-temperature sublethal temperatures for the 15 materials were -6.88,-5.95,-5.37,-5.16,-4.64,-4.21,-3.93,-2.56,-2.48,-2.38,-2.25,-1.98,-1.13,-1.09 ℃ and -0.92 ℃.Among these，WJ1 exhibited the lowest low-temperature sublethal temperature，while WJ12 showed the highest.Through comprehensive evaluation，the 15 materials were classified into 2 cold-tolerant types，9 moderately cold-tolerant types，and 4 cold-sensitive types.Among these，2 cold-tolerant materials，WJ1 and WJ6，were selected.A preliminary quantitative regression equation predicting turnip seedling cold tolerance was obtained Y=-0.489+0.081X1+0.178X2+0.181X3.
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备注/Memo
第一作者简介：魏书华(2001-)，男，硕士研究生，研究方向为植物生理生态与逆境生理。E-mail:ahuaw01@163.com.责任作者：海梅荣(1973-)，女，博士，教授，硕士生导师，现主要从事植物生理生态等研究工作。E-mail:2250029499@qq.com.基金项目：西藏昌都光宇利民药业有限责任公司委托横向课题资助项目（KX132020033）。收稿日期：2025-07-29