[1]郭瑾,吴永成,覃彩芳,等.黑果枸杞LrWRKY44的克隆、生物信息学及差异表达分析[J].北方园艺,2026,(5):21-29.[doi:10.11937/bfyy.20252223]
　GUO Jin,WU Yongcheng,QIN Caifang,et al.Cloning，Bioinformatics and Expression Analysis of LrWRKY44 in Lycium ruthenicum Murr.[J].Northern Horticulture,2026,(5):21-29.[doi:10.11937/bfyy.20252223]
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黑果枸杞LrWRKY44的克隆、生物信息学及差异表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年5 页码: 21-29 栏目: 出版日期: 2026-03-15
Title:
Cloning，Bioinformatics and Expression Analysis of LrWRKY44 in Lycium ruthenicum Murr.
文章编号:
1001-0009(2026)05-0021-09
作者:
郭瑾1; 吴永成1; 覃彩芳1; 刘慧1; 王静1; 2
（1.北方民族大学 生物科学与工程学院，宁夏 银川 750021；2.北方民族大学 经济林遗传改良创新团队，宁夏 银川 750021）
Author(s):
GUO Jin1; WU Yongcheng1; QIN Caifang1; LIU Hui1; WANG Jing1; 2
(1.College of Biological Science and Engineering,North Minzu University,Yinchuan,Ningxia 750021；2.Innovative Team for Genetic Improvement of Economic Forests,North Minzu University,Yinchuan，Ningxia 750021)
关键词:
黑果枸杞; LrWRKY44; 生物信息学分析; 表达特性
Keywords:
Lycium ruthenicum Murr.; LrWRKY44; bioinformatics analysis; expression analysis
分类号:
S853.75
DOI:
10.11937/bfyy.20252223
文献标志码:
A
摘要:
以黑果枸杞的果实、根、茎、叶、花为试材,通过生物信息学工具分析了黑果枸杞LrWRKY44的结构，并通过RT-qPCR分析黑果枸杞在不同组织及不同发育时期的表达模式，以期初步探究LrWRKY44 在黑果枸杞生长发育中的生物学功能。结果表明：LrWRKY44在黑果枸杞的根、茎、叶、花及果实中均有表达，其中在果实中的表达量最高。随着果实的发育，LrWRKY44的表达量逐渐增加，在成熟期达到峰值。这一表达模式与花青素含量的积累趋势相吻合。LrWRKY44可能与黑果枸杞花青素的生物合成与积累密切相关，对黑果枸杞果实色泽形成具有重要调控作用。
Abstract:
Taking Lycium ruthenicum fruit，root，stem，leaf，and flower as the experimental materials,the structure of LrWRKY44 was analyzed using bioinformatics tools，and its expression patterns in different tissues and at different developmental stages were determined by RT-qPCR，in order to preliminarily explore its biological function in the growth and development of L.ruthenicum.The results showed that LrWRKY44 was expressed in all parts of L.ruthenicum，including roots，stems，leaves，flowers，and fruits，with the highest expression level found in fruits.As the fruit matures，the expression of LrWRKY44 gradually increases，peaking at maturity，which aligns with the accumulation trend of anthocyanin content.The results indicated that LrWRKY44 might be closely related to the biosynthesis and accumulation of anthocyanins in L.ruthenium and played an important regulatory role in the formation of fruit color.

参考文献/References:

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备注/Memo

第一作者简介：郭瑾（1994-），女，硕士研究生，研究方向为植物次生代谢产物调控。E-mail：1282524504@qq.com.责任作者：王静（1984-），女，博士，副教授，现主要从事植物次生代谢产物调控等研究工作。E-mail：wangjing_imu2@163.com.基金项目：北方民族大学研究生创新资助项目（YCX24405）；国家自然科学基金资助项目（32160392）；宁夏回族自治区重点研发计划资助项目（2021BEF02002）。收稿日期：2025-06-17

更新日期/Last Update: 2026-03-16