[1]郑群,陈芮,李茂生,等.植物激素调控薄壳山核桃容器苗生长与根系代谢组[J].北方园艺,2025,(17):58-68.[doi:10.11937/bfyy.20250881]
　ZHENG Qun,CHEN Rui,LI Maosheng,et al.Phytohormone Regulate the Growth of Pecan Container Seedlings and the Root Metabolome[J].Northern Horticulture,2025,(17):58-68.[doi:10.11937/bfyy.20250881]
点击复制

植物激素调控薄壳山核桃容器苗生长与根系代谢组

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年17 页码: 58-68 栏目: 出版日期: 2025-09-15
Title:
Phytohormone Regulate the Growth of Pecan Container Seedlings and the Root Metabolome
文章编号:
1001-0009(2025)17-0058-11
作者:
郑群1; 陈芮1; 李茂生1; 宋志光1; 万学康1; 刘嘉2
（1.滁州市润森林业投资开发有限责任公司，安徽 滁州 219039；2.滁州学院，安徽 滁州 219039）
Author(s):
ZHENG Qun1; CHEN Rui1; LI Maosheng1; SONG Zhiguang1; WAN Xuekang1; LIU Jia2
(1.Chuzhou Runsen Forestry Investment and Development Co.Ltd.，Chuzhou,Anhui 219039；2.Chuzhou University，Chuzhou,Anhui 219039)
关键词:
山核桃幼苗; 植物激素; 根系; 代谢
Keywords:
pecan seedlings; phytohormone; root; metabolism
分类号:
S 664.1
DOI:
10.11937/bfyy.20250881
文献标志码:
A
摘要:
以薄壳山核桃容器苗为试材，采用非靶向代谢组学，研究了6种植物植物激素处理对薄壳山核桃容器苗地径、苗高、细根形态和根系代谢的影响,以期为容器苗栽培提供参考依据。结果表明：GA3及其组合处理显著促进了地径和苗高的生长，而IBA处理抑制了苗高生长，IBA组合处理（IBA+NAA+IAA）抑制地径。与IAA相关的处理显著增加了总根长和根表面积，其中IAA+NAA+IBA处理增加了平均根直径。代谢组学分析显示，在苯丙烷生物合成、ABC 转运蛋白、苯丙氨酸、酪氨酸和色氨酸生物合成、硫胺素代谢、黄酮类生物合成、单环β-内酰胺生物合成、苯丙氨酸代谢、氰基氨基酸代谢和异喹啉生物碱生物合成等代谢途径中存在显著差异。
Abstract:
Taking container seedlings of pecan as test materials，the effects of six plant hormone treatments on ground diameter，seedling height，fine root morphology，and root metabolism were investigated employing non-targeted metabolomics,in order to provide reference for container seeding cultivation.The results showed that GA3 and its combination (GA3+NAA+IAA) significantly promoted stem diameter and height growth，while IBA treatment inhibited height and IBA combination (IBA+NAA+IAA) reduced stem diameter.IBA-related treatments significantly enhanced total root length and surface area，with IBA+NAA+IAA treatment increasing average diameter.Metabolomics revealed significant differences in metabolic pathways including phenylpropanoid biosynthesis，ABC transporters，phenylalanine，tyrosine and tryptophan biosynthesis，thiamine metabolism，flavonoid biosynthesis，monobactam biosynthesis，phenylalanine metabolism,cyanoamino acid metabolism and isoquinoline alkaloid biosynthesis.This study elucidates the physiological mechanisms of phytohormone regulation in pecan seedling growth.

参考文献/References:

［1］LIU Y,WANG Q,DING Y,et al.Effects of external ABA,GA3 and NAA on the tiller bud outgrowth of rice is related to changes in endogenous hormones［J］.Plant Growth Regulation,2011,65(2):247-254.［2］FERNIE A R,SCHAUER N.Metabolomics-assisted breeding:A viable option for crop improvement?［J］.Trends in Genetics,2009,25(1):39-48.［3］SULPICE R,PYL E T,ISHIHARA H,et al.Starch as a major integrator in the regulation of plant growth［J］.Proceedings of the National Academy of Sciences of the United States of America,2009,106(25):10348-10353.［4］TOHGE T,WATANABE M,HOEFGEN R,et al.Shikimate and phenylalanine biosynthesis in the green lineage［J］.Frontiers in Plant Science,2013,4:62.［5］MATSUDA F,HIRAI M Y,SASAKI E,et al.AtMetExpress development:A phytochemical atlas of Arabidopsis development［J］.Plant Physiology,2010,152(2):566-578.［6］薛婷婷,刘嘉,沈永宝,等.温度对薄壳山核桃种子萌发的影响及其机制初探［J］.中南林业科技大学学报,2017,37(11):42-50.［7］HEDDEN P,THOMAS S G.Gibberellin biosynthesis and its regulation［J］.Biochemical Journal,2012,444(1):11-25.［8］SUN T P,GUBLER F.Molecular mechanism of gibberellin signaling in plants［J］.Annual Review of Plant Biology,2004,55:197-223.［9］FRIML J,PALME K.Polar auxin transport-old questions and new concepts?［J］.Plant Molecular Biology,2002,49(3):273-284.［10］ALONI R,ALONI E,LANGHANS M,et al.Role of auxin in regulating Arabidopsis flower development［J］.Planta,2006,223(2):315-328.［11］LUDWIG-MLLER J.Indole-3-butyric acid in plant growth and development［J］.Plant Growth Regulation,2000,32(2):219-230.［12］STADEN J V，ZAZIMALOVA E，GEORGE E F.Plant growth regulators II：Cytokinins，their analogues and antagonists［J］.The Annals of Thoracic Surgery，2006，82(6)：2031-2036.［13］OKUSHIMA Y,FUKAKI H,ONODA M,et al.ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis［J］.The Plant Cell,2007,19(1):118-130.［14］OVERVOORDE P,FUKAKI H,BEECKMAN T.Auxin control of root development［J］.Cold Spring Harbor Perspectives in Biology,2010,2(6):a001537.［15］de KLERK G J,van DER KRIEKEN W,de JONG J C.Review the formation of adventitious roots:New concepts,new possibilities［J］.In Vitro Cellular & Developmental Biology-Plant,1999,35(3):189-199.［16］HEDDEN P.Gibberellin metabolism and its regulation［J］.Journal of Plant Growth Regulation,2001,20(4):317-318.［17］UBEDA-TOMS S,SWARUP R,COATES J,et al.Root growth in Arabidopsis requires gibberellin/DELLA signalling in the endodermis［J］.Nature Cell Biology,2008,10(5):625-628.［18］PEER W A,BROWN D E,TAGUE B W,et al.Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis［J］.Plant Physiology,2001,126(2):536-548.［19］BHALERAO R P,EKLF J,LJUNG K,et al.Shoot-derived auxin is essential for early lateral root emergence in Arabidopsis seedlings［J］.Plant Journal,2002,29(3):325-332.［20］GEISLER M,MURPHY A S.The ABC of auxin transport:The role of p-glycoproteins in plant development［J］.FEBS Letters,2006,580(4):1094-1102.［21］KRETZSCHMAR T,BURLA B,LEE Y,et al.Functions of ABC transporters in plants［J］.Essays in Biochemistry,2011,50(1):145-160.［22］SWARUP R,KRAMER E M,PERRY P,et al.Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal［J］.Nature Cell Biology,2005,7(11):1057-1065.［23］GRUNEWALD W,de SMET I,LEWIS D R,et al.Transcription factor WRKY23 assists auxin distribution patterns during Arabidopsis root development through local control on flavonol biosynthesis［J］.Proceedings of the National Academy of Sciences of the United States of America,2012,109(5):1554-1559.［24］NGUYEN N H,KIM J H,KWON J,et al.Characterization of Arabidopsis thaliana FLAVONOL SYNTHASE 1 (FLS1)-overexpression plants in response to abiotic stress［J］.Plant Physiology and Biochemistry,2016,103:133-142.［25］XU J,YU Y,SHI R,et al.Organ-specific metabolic shifts of flavonoids in Scutellaria baicalensis at different growth and development stages［J］.Molecules,2018,23(2):428.［26］LIU W C,SONG R F,ZHENG S Q,et al.Coordination of plant growth and abiotic stress responses by tryptophan synthase β subunit 1 through modulation of tryptophan and ABA homeostasis in Arabidopsis［J］.Molecular Plant,2022,15(6):973-990.［27］PASCUAL M B,EL-AZAZ J,de LA TORRE F N,et al.Biosynthesis and metabolic fate of phenylalanine in conifers［J］.Frontiers in Plant Science,2016,7:1030.［28］CHENG Q,ZENG J,LE J.Process of cell biology of isoquinoline alkaloid biosynthesis,transport and storage［J］.Chinese Bulletin of Botany,2014,49(6):720.［29］SAMANANI N,PARK S U,FACCHINI P J.Cell type-specific localization of transcripts encoding nine consecutive enzymes involved in protoberberine alkaloid biosynthesis［J］.The Plant Cell,2005,17(3):915-926.［30］YOSHIDA H,NAGATA M,SAITO K,et al.Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases［J］.BMC Plant Biology,2005(5):14.

相似文献/References:

[1]刘存宏,张富娥.植物激素在山楂上的应用研究[J].北方园艺,2013,37(10):18.
　LIU Cun-hong,ZHANG Fu-e.Application of Plant Hormone to Hawthorn[J].Northern Horticulture,2013,37(17):18.
[2]赵丽华.洋葱组培快繁技术研究[J].北方园艺,2013,37(18):86.
　ZHAO Li-hua.Study on the Tissue Culture and Rapid Propagation Technology of Allium cepa L.[J].Northern Horticulture,2013,37(17):86.
[3]黄 颖,胡 磊,谷 晴,等.“大马士革”玫瑰茎尖组培快繁技术研究[J].北方园艺,2014,38(03):104.
　HUANG Ying,HU Lei,GU Qing,et al.Study on Tissue Culture and Rapid Propagation on Stem Tip of Rosa damascus[J].Northern Horticulture,2014,38(17):104.
[4]智利婷,洪培培,陈洪伟,等.外源植物激素对一串红侧芽生长的影响[J].北方园艺,2013,37(07):52.
　ZHI Li-ting,HONG Pei-pei,CHEN Hong-wei,et al.Effects of Applying Exogenous Plant Hormone on Lateral Bud Growth of Salvia splendens[J].Northern Horticulture,2013,37(17):52.
[5]徐宗才,马明呈,田丰,等.基质及植物激素对枸杞嫩枝扦插效果的影响[J].北方园艺,2012,36(16):174.
　XU Zong-cai,MA Ming-cheng,TIAN Feng,et al.The Effect of Different Substrates and Plant Hormones on Chinese Wolfberry Shoots Cuttings[J].Northern Horticulture,2012,36(17):174.
[6]金建邦,祝遵凌.桦木科植物组织培养研究进展[J].北方园艺,2013,37(23):202.
　JIN Jian-bang,ZHU Zun-ling.Research Progress of Tissue Culture of Betulaceae[J].Northern Horticulture,2013,37(17):202.
[7]王萍,沈晓婷,王罡,等.植物激素对黄瓜愈伤组织形成与芽分化的影响[J].北方园艺,2013,37(24):84.
　WANG Ping,SHEN Xiao-ting,WANG Gang,et al.Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber[J].Northern Horticulture,2013,37(17):84.
[8]黄铭慧,冯舒涵,罗姗姗,等.外源植物激素对园艺产品品质形成的调控作用[J].北方园艺,2015,39(12):178.[doi:10.11937/bfyy.201512047]
　HUANG Ming-hui,FENG Shu-han,LUO Shan-shan,et al.Regulation Effect of Exogenous Plant Hormone on the Quality Formation of Horticultural Products[J].Northern Horticulture,2015,39(17):178.[doi:10.11937/bfyy.201512047]
[9]周 　 恒.荷 包 花 离 体 繁 殖 研 究 初 报[J].北方园艺,2009,33(05):0.[doi:10.11937/bfyy.200905036]
　ZHOU Heng.Reports of The Dicentra Pusilla Exsomatize Reproduction[J].Northern Horticulture,2009,33(17):0.[doi:10.11937/bfyy.200905036]
[10]徐宗才,马明呈,张义英,等.植物激素处理对枸杞硬枝插穗的影响[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108067]
　XU Zong-eai. MA Ming-cheng,ZHANG Yi-ying,CHEN Ying-chun,et al.Study on the Plant Hormone Treatments of Wolfberry Hardwood Cuttings[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.201108067]

备注/Memo

第一作者简介：郑群（1978-），男，本科，中级经济师，现主要从事林业经济与薄壳山核桃栽培等研究工作。E-mail：czuuzq@139.com.责任作者：刘嘉(1988-),男,博士,副教授,现主要从事林木种苗等研究工作。E-mail：liujia6228701@outlook.com.基金项目：安徽省教育厅优秀青年基金资助项目（2022AH030111）；安徽省教育厅重点资助项目 （2023AH051628）。收稿日期：2025-03-11

更新日期/Last Update: 2025-10-10