WU Qiang,ZHANG Xiaoshen,CAO Hui,et al.Transcriptomic Analysis of Lonicera japonica in Response to Powdery Mildew Infection[J].Northern Horticulture,2025,(13):132-139.[doi:10.11937/bfyy.20250303]
金银花响应白粉病侵染的转录组学分析
- Title:
- Transcriptomic Analysis of Lonicera japonica in Response to Powdery Mildew Infection
- 文章编号:
- 1001-0009(2025)13-0132-08
- Keywords:
- Lonicera japonica; powdery mildew; transcriptome
- 分类号:
- S 853.75
- 文献标志码:
- A
- 摘要:
- 以金银花（Lonicera japonica）品种“封花1号”为试材，采用高通量测序的方法对金银花进行转录组测序分析，研究了忍冬叉丝壳菌（Erysiphe lonicerae var.lonicera）引起的白粉病对金银花生长发育的影响，筛选抗白粉病相关基因和转录因子，并对筛选到的转录组进行qRT-PCR验证，以期挖掘金银花抵御白粉病分子机制，为金银花的遗传改良和品种选育提供参考依据。结果表明：包括物-病原菌互作、植物激素信号转导、类黄酮生物合成和丙酮酸代谢在内的多个代谢通路以及多个差异基因参与到金银花抵抗白粉病菌过程中，也找到LjWRKY13、LjWRKY27、LjERF14、LjMYB20和LjNTL5这些转录因子是金银花响应白粉病菌侵染的抗病转录因子。
- Abstract:
- Taking the Lonicera japonica variety ‘Fenghua No.1’ as the experimental material，the transcriptome sequencing analysis of Lonicera japonica was conducted by high-throughput sequencing to study the effect of powdery mildew caused by Erysiphe lonicerae var.lonicera on the growth and development of Lonicera japonica,screen the genes and transcription factors related to resistance to powdery mildew，and verify the screened transcriptome by qRT-PCR，in order to explore the molecular mechanism of Lonicera japonica′s resistance to powdery mildew and provide reference for the genetic improvement and variety breeding of Lonicera japonica.The results showed that multiple metabolic pathways，including drug-pathogen interactions，plant hormone signal transduction，flavonoid biosynthesis and pyruvate metabolism，as well as multiple differentially expressed genes，were involved in the process of Lonicera japonica′s resistance to powdery mildew pathogen.It was also found that the transcription factors LjWRKY13，LjWRKY27，LjERF14，LjMYB20 and LjNTL5 were the disease-resistant transcription factors of Lonicera japonica in response to powdery mildew infection.
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备注/Memo
第一作者简介：吴强（1997-），男，硕士，助理研究员，现主要从事甘薯遗传育种与金银花抗病机理等研究工作。E-mail：786772455@qq.com.责任作者：张晓申（1976-），男，博士，副研究员，现主要从事甘薯遗传育种及栽培与中药材新品种选育及栽培技术等研究工作。E-mail：E-mail：nkszyc@126.com.基金项目：国家现代农业产业技术体系资助项目（CARS-21）；河南省林木科技兴林资助项目（豫财环资［2023］60号）。收稿日期：2025-02-05