[1]陈苏丹,李秀珍,李学强,等.核桃枝皮多酚提取工艺优化及提取液抑菌研究[J].北方园艺,2025,(14):49-55.[doi:10.11937/bfyy.20245222]
　CHEN Sudan,LI Xiuzhen,LI Xueqiang,et al.Study on Optimization of Polyphenol Extraction From Twig Bark of Walunt and Antibacterial Activity of the Extract[J].Northern Horticulture,2025,(14):49-55.[doi:10.11937/bfyy.20245222]
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核桃枝皮多酚提取工艺优化及提取液抑菌研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年14 页码: 49-55 栏目: 出版日期: 2025-07-30
Title:
Study on Optimization of Polyphenol Extraction From Twig Bark of Walunt and Antibacterial Activity of the Extract
文章编号:
1001-0009(2025)14-0049-07
作者:
陈苏丹; 李秀珍; 李学强; 王沛京; 郑伟; 刘永康
（河南科技大学 园艺与植物保护学院，河南 洛阳 471003）
Author(s):
CHEN Sudan; LI Xiuzhen; LI Xueqiang; WANG Peijing; ZHENG Wei; LIU Yongkang
(College of Horticulture and Plant Protection，Henan University of Science and Technology，Luoyang,Henan 471003)
关键词:
核桃; 多酚; 超声辅助提取; 正交实验; 抑菌
Keywords:
Juglans regia L.; polyphenols; ultrasonic-assisted extraction; orthogonal test; antifungal activity
分类号:
S 664.1
DOI:
10.11937/bfyy.20245222
文献标志码:
A
摘要:
以核桃枝皮为试材，采用单因素试验和正交实验法，优化核桃枝皮多酚的超声辅助提取工艺，并测定不同浓度的多酚提取液对玉米大斑病原菌（Exsero turcicum）、辣椒疫霉（Phytophthora capsici）、兰花炭疽菌（Colletotrichum orchidearum）、小麦茎基腐镰刀菌（Fusarium graminearum）的抑制效果，以期为核桃的深入开发提供参考依据。结果表明：核桃枝皮多酚的最优提取工艺为乙醇体积分数40%、液料比1∶20 g·mL-1、超声时间30 min、超声温度40 ℃，在该工艺条件下，核桃枝皮多酚含量为0.72 mg·g-1。核桃枝皮多酚提取液对4种病原真菌均具有一定的抑制作用，当多酚溶液浓度为0.36 g·mL-1时，对兰花炭疽菌的抑菌率达到91.64%，对小麦茎基腐镰刀菌和玉米大斑的抑菌率分别为77.47%和66.64%，对辣椒疫霉的抑菌率为31.41%，表明核桃枝皮多酚具有良好的抑菌能力，可作为天然的抑菌剂进一步的开发和利用。
Abstract:
Taking twig bark of walunt as the test material，polyphenols was extracted by ultrasonic-assisted extraction technology base on orthogonal test，and antifungal activity of the different concentrations of polyphenols extracts on Exsero turcicum,Phytophthora capsici,Colletotrichum orchidearum and Fusarium graminearum were measured，in order to provide reference for the indepth development of walnuts.The optimal extraction process of polyphenols from twig bark of walunt were as follows，40% ethanol volume fraction，1∶20 g·mL-1 of material to liquid，30 minutes of ultrasonic time and 40 ℃ ultrasonic temperature.Under this optimal extraction condition，the content of polyphenols from twig bark of walunt was 0.72 mg·g-1.The results showed that the polyphenols extracts from twig bark of walunt have different range activities to four fungus.The maximum inhibiting rate could be reached 91.64% to Colletotrichum orchidearum，77.47% and 66.64% corresponding to Fusarium graminearum and Exsero turcicum，31.41% to Phytophthora capsici with the concentration of polyphenols as 0.36 g·mL-1，which illustrated that the polyphenols extracts from twig bark of walunt had the outstanding antifungal activity and could be developed and utilized deeply as the natural antifungal agent in the future.

参考文献/References:

［1］许海峰,陈新,张士刚,等.基于代谢组学分析‘秋香’核桃贮藏期间脂肪酸的变化［J］.园艺学报,2021,48(11):2161-2170.［2］余启明,谢代祖,蔡锦源,等.19种不同产地核桃的营养成分及脂肪酸的分析比较研究［J］.食品研究与开发,2020,41(2):149-156.［2］张琴,康明丽,赵茹,等.青皮核桃采后腐烂病病原菌分离与鉴定［J］.北方园艺,2024(4):74-80.［3］COSMULESCU S，TRANDAFIR I，ACHIM G，et al.Phenolics of green husk in mature walnut fruits［J］.Notulae Botanicae Horti Agrobotanici Cluj-Napoca,2010，38(1):53-56.［4］董科,冷云,何方婷,等.植物多酚及其提取方法的研究进展［J］.食品工业科技,2019,40(20):326-330.［5］ZAMANI-GARMSIRI F,EMAMGHOLIPOUR S,RAHMANI FARD S,et al.Polyphenols:Potential anti-inflammatory agents for treatment of metabolic disorders［J］.Phytotherapy Research,2022,36(1):415-432.［6］PRABAKARAN M,KIM S H,SASIREKA A,et al.Polyphenol composition and antimicrobial activity of various solvent extracts from different plant parts of Moringa oleifera［J］.Food Bioscience,2018(26):23-29.［7］王智宸.环剥及生长调节剂对核桃枝条发育和碳氮代谢物积累的影响［D］.阿拉尔：塔里木大学,2024.［8］邱明清,韦思洁,李灵犀,等.辣木叶多酚的提取与分离方法研究进展［J］.沈阳药科大学学报,2022,39(8)：1036-1046.［9］王长雷,张文娥,潘学军.核桃雄花中总多酚提取工艺的研究［J］.食品工业,2015,36(1):77-81.［10］路振康,吴庆智,张建,等.核桃青皮提取物的多酚含量、体外抗氧化和抗菌活性的评价［J］.食品科学,2023,44(3):79-87.［11］杜慧平,赵瑞芬,燕照玲.核桃青皮活性物质提取方法及其抑菌作用研究进展［J］.河南农业科学,2017,46(9):1-5.［12］王纪辉,耿阳阳,刘亚娜,等.不同溶剂浸提下核桃果实不同部位多酚物质响应及其组成［J］.南京师大学报(自然科学版),2022,45(3):35-45.［13］王纪辉,耿阳阳,胡伯凯,等.核桃分心木多酚物质响应及组成与抗氧化能力探究［J］.西南大学学报(自然科学版),2023,45(8):83-94.［14］刘静,黄慧福,刘继华,等.响应面优化核桃分心木多酚超声辅助提取工艺［J］.食品研究与开发,2020,41(23):155-160,191.［15］王纪辉,何佳丽,耿阳阳,等.核桃叶多酚提取及其组成和抗氧化能力探究［J］.中国粮油学报,2023,38(4):104-112.［16］曹文利,薛雨菲,杨永兴,等.核桃青皮不同溶剂提取物抗氧化及抑菌活性比较［J］.中国酿造,2019,38(1):149-153.［17］王倩宁,杜彦慧,张楠,等.核桃青皮不同溶剂提取物抗氧化和抑菌活性对比研究［J］.天津农业科学,2024,30(2):62-67,73.［18］ROMERO-DEZ R,MATOS M,RODRIGUES L,et al.Microwave and ultrasound pre-treatments to enhance anthocyanins extraction from different wine lees［J］.Food Chemistry,2019,272:258-266.［19］张一格.核桃不同部位多酚的比较、消化特性及稳定性研究［D］.马鞍山：安徽工业大学,2021.［20］杨雨乐.五种药用植物提取物抑菌活性及益母草抑菌活性成分研究［D］.南昌:江西农业大学,2023.［21］刘丽金.核桃酚酸类成分抗氧化活性的初步研究［D］.大理:大理大学,2018.［22］沈碧寒,陈嘉颖,李治康,等.响应面法优化核桃青皮多酚提取工艺及其自由基清除活力的研究［J］.粮食与油脂,2020,33(10):117-120.［23］杨郑州,李曦,谢晓娜.芒果皮多酚提取工艺的优化及抗氧化能力分析［J］.江西农业学报,2023,35(5):103-108.［24］苏学友.核桃青皮提取物抑菌活性研究［D］.乌鲁木齐：新疆农业大学,2008.［25］翟梅枝,王磊,何文君,等.核桃青皮乙醇提取物抑菌活性研究［J］.西北植物学报,2009,29(12):2542-2547.［26］马良进,吴美卿,苏秀,等.山核桃外果皮提取物活体抑菌活性［J］.浙江林学院学报,2009,26(5):620-624.［27］左龙亚.柑橘亚属植物果皮多酚类物质提取及其抗氧化、抑菌活性检测［D］.重庆:西南大学,2018.［28］朱奇奇,张杰,谢平,等.八角多酚提取物对植物病原真菌的抑制作用研究［J］.中国调味品,2017,42(5):5-8.［29］谢水香,雷彩燕.油茶叶多酚的提取及其对常见植物病原真菌抑制作用研究［J］.天然产物研究与开发,2023,35(9):1562-1568,1649.

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备注/Memo

第一作者简介：陈苏丹（1984-），女，博士，副教授，现主要从事园艺植物种质资源评价与创新等研究工作。E-mail：elims2006@163.com.责任作者：李秀珍（1969-），女，博士，教授，博士生导师，现主要从事园艺植物种质资源创新及高效栽培技术等研究工作。E-mail：lxzly737@163.com.基金项目：国家自然科学基金资助项目（62271192）；河南省科技研发计划联合基金（应用攻关类）资助项目（232103810040）。收稿日期：2024-12-26

更新日期/Last Update: 2025-07-28