[1]秦佳乐,宋立君,张芹,等.EMS诱导皂荚突变体的筛选及ISSR分析[J].北方园艺,2025,(11):32-39.[doi:10.11937/bfyy.20244314]
　QIN Jiale,SONG Lijun,ZHANG Qin,et al.Screening and ISSR Analysis of EMS-induced Gleditsia sinensis Mutants[J].Northern Horticulture,2025,(11):32-39.[doi:10.11937/bfyy.20244314]
点击复制

EMS诱导皂荚突变体的筛选及ISSR分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年11 页码: 32-39 栏目: 出版日期: 2025-06-16
Title:
Screening and ISSR Analysis of EMS-induced Gleditsia sinensis Mutants
文章编号:
1001-0009(2025)11-0032-08
作者:
秦佳乐1; 宋立君1; 张芹2; 郑建伟1; 马国栋1; 李保会1
（1.河北农业大学 林学院，河北 保定 071000；2.河北农业大学 园林与旅游学院，河北 保定 071000）
Author(s):
QIN Jiale1; SONG Lijun1; ZHANG Qin2; ZHENG Jianwei1; MA Guodong1; LI Baohui1
(1.College of Forestry，Hebei Agricultural University，Baoding，Hebei 071000；2.College of Landscape Architecture and Tourism，Hebei Agricultural University，Baoding，Hebei 071000)
关键词:
甲基磺酸乙酯诱变; 皂荚; 突变体库; ISSR分子标记
Keywords:
EMS mutagenesis; Gleditsia sinensis; mutant library; ISSR molecular markers
分类号:
S 722.3+5
DOI:
10.11937/bfyy.20244314
文献标志码:
A
摘要:
以皂荚萌动种子为试材，采用不同浓度的EMS溶液和不同的处理时间进行诱变处理，通过形态学、ISSR分子鉴定筛选皂荚突变体，以期为进一步挖掘皂荚基因功能和表型之间的关系及品种遗传改良提供参考依据。结果表明：随着EMS处理浓度和时间的增加，皂荚萌动种子的发芽率、发芽势、成苗率受到抑制，0.6% EMS处理12 h为皂荚种子最佳诱变处理，在该处理组合下，变异率为47.33%，主要为叶色、叶型、植株高矮等3种变异类型。12 h处理下的半致死剂量为0.581 7%。ISSR分析结果表明，19条引物共扩增出173个等位基因位点，多态性位点171个，多态性比率为99.42%。7个表型变异植株和对照的遗传相似系数变化范围为0.097 1～1.000 0。
Abstract:
Taking germinated seeds of Gleditsia sinensis as experimental materials and treated with EMS at different concentrations and durations to induce mutagenesis.Morphological screening and ISSR molecular marker analysis were conducted to identify mutants，in order to provide reference for further exploring the relationship between the gene function and phenotype of Gleditsia sinensis and for the genetic improvement of varieties.The results showed that increasing EMS concentration and treatment duration significantly inhibited seed germination rate，germination potential，and seedling survival rate.The optimal mutagenic treatment was identified as 0.6% EMS for 12 hours，which yielded a mutation frequency of 47.33%.The primary phenotypic variations included alterations in leaf color，leaf morphology，and plant height.The semi-lethal dose (LD50) for the 12 hours treatment was calculated to be 0.581 7%.ISSR analysis using 19 primers amplified 173 allelic loci，among which 171 were polymorphic，resulting in a polymorphism rate of 99.42%.The genetic similarity coefficients between seven phenotypically variant plants and the control group ranged from 0.097 1 to 1.000 0.

参考文献/References:

［1］刘富华,王秀荣,赵杨,等.皂荚种皮结构和休眠特性研究［J］.种子,2024,43(6):91-99.［2］韩丽君.山西省皂荚种实性状变异与环境因子的关系及优良种质筛选［J］.东北林业大学学报,2024,52(7):13-18.［3］王召伟,张玉华,张霞,等.皂荚树的应用价值及综合开发研究［J］.中国林副特产,2021(5):80-81,84.［4］李灵会.皂荚经济利用价值与市场前景分析及发展存在的问题及策略［J］.现代园艺,2018(6):22-23.［5］李佳秋,田佳赫,于丽娜,等.不同施氮量对皂荚幼苗生长及光合特性的影响［J］.林业与生态科学,2024,39(1):28-33.［6］DHAKSHANAMOORTHY D,SELVARAJ R,CHIDAMBARAM A.Utility of RAPD marker for genetic diversity analysis in gamma rays and ethyl methane sulphonate (EMS)-treated Jatropha curcas plants［J］.Comptes Rendus Biologies,2015,338(2):75-82.［7］ACANDA Y,MARTNEZ ,PRADO M J,et al.EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine［J］.Plant Cell Reports,2014,33(3):471-481.［8］安佰义,丁晓丽,吴双,等.EMS诱变白桦种子发芽的条件筛选［J］.分子植物育种,2019,17(18):6073-6079.［9］刘艳菊,徐玉芬,于钊妍,等.EMS诱变对油茶种子萌发及幼苗生长的影响［J］.热带农业科学,2022,42(9):39-43.［10］张慧,阮明菊,王韦,等.辣木甲基磺酸乙酯(EMS)诱变及表型变异的初步研究［J］.云南农业大学学报(自然科学),2022,37(2):302-310.［11］张蕊,齐钦辉,朱文瑛,等.皂荚ISSR-PCR反应体系的建立与优化［J］.林业与生态科学,2024,39(3):264-272.［12］谢玲玲,周火强,弭宝彬,等.EMS诱变技术研究概况及应用进展［J］.湖南农业科学,2020(6):92-95.［13］李志萌,崔丽朋,万秀娟,等.EMS诱变处理对洋葱种子萌发的影响及其变异表型的观察［J］.北方园艺,2024(18):1-6.［14］钟程,田鑫,唐其蔚,等.EMS诱变对不结球白菜愈伤效果及其抗热性的影响［J］.耕作与栽培,2023,43(1):30-34,42.［15］吴正景,职钤华,刘素娟,等.化学诱变提高植物抗逆性的研究进展［J］.核农学报,2024,38(2):274-281.［16］宋兰芳,华明艳,崔少杰,等.EMS诱变对不同萌发状态草莓种子发芽率的影响［J］.贵州农业科学,2023,51(7):74-78.［17］喇黑麦,董文科,马福钦,等.EMS诱变对鹰嘴紫云英种子萌发及幼苗表型特征的影响［J］.草原与草坪,2024,44(3):116-124.［18］罗文龙,陈立凯,王慧,等.“神舟八号” 搭载‘航恢173’种子的当代生物效应及SSR分析［J］.中国农学通报,2014,30(15):11-16.［19］杨绍华,刘华清,王锋.水稻温敏雄性不育突变体mh86s的鉴定及基因克隆［J］.福建农业科技,2020,51(1):1-5.［20］LIU Y,LI Y,WANG A,et al.Enhancing cold resistance in Banana (Musa spp.) through EMS-induced mutagenesis,L-Hyp pressure selection:Phenotypicalterations,biomasscomposition,and transcriptomic insights［J］.BMC Plant Biology,2024,24(1):101.［21］陈星灼,张星星,陈俊锟,等.EMS诱变对甘蓝型油菜主要农艺性状的影响［J］.贵州农业科学,2022,50(9):1-6.［22］尹丽娟,王春霞,李陈浩,等.西瓜EMS诱导的突变体筛选及表型分析［J］.园艺学报,2023,50(11):2401-2416.［23］寇紫倩,金梦然,刘雅兰,等.甲基磺酸乙酯处理对紫丁香种子萌发及幼苗表型的影响［J］.新疆农业大学学报,2022,45(4):285-291.［24］MACDONALD M S,ABBRIANO R M,HERDEAN A,et al.Random mutagenesis of Phaeodactylum tricornutum using ultraviolet,chemical,and X-radiation demonstrates the need for temporal analysis of phenotype stability［J］.Scientific Reports,2023,13(1):22385.［25］KIANI S,KAHRIZI D,VARMIRA K,et al.Molecular and biochemical evaluation of ethyl methanesulfonate-induced mutant lines in Camelina sativa L.［J］.Iranian Journal of Biotechnology,2022,20(1):e2948.［26］刘丽丽,刘汝明,刘燕,等.基于ISSR标记的12份樱花种质亲缘关系分析［J］.分子植物育种,2024,22(10):3247-3252.［27］姚强,张元祺,李文刚,等.基于ISSR分子标记的羊肚菌山东主栽菌株遗传多样性分析［J］.食用菌,2024,46(4):21-24.［28］张运峰,屈安姜,付薪蒙.基于PAGE检测技术的平菇ISSR-PCR体系的建立和优化［J］.北方园艺,2017(2):91-95.［29］朱晨,张舒婷,常笑君,等.福建省53份茶树种质资源遗传多样性ISSR分析［J］.热带作物学报,2017,38(7):1303-1310.［30］刘靖,赵双双,古敬锋,等.纤花香茶菜EMS突变体库构建及ISSR与SSR分析［J］.种子,2024,43(4):17-26.

相似文献/References:

[1]张萍,姜景彬,杨欢欢,等.番茄EMS突变体库的构建及表型分析[J].北方园艺,2019,43(07):1.[doi:10.11937/bfyy.20183698]
　ZHANG Ping,JIANG Jingbin,YANG Huanhuan,et al.Creation and Phenotypic Analysis of Tomato EMS Mutant Library[J].Northern Horticulture,2019,43(11):1.[doi:10.11937/bfyy.20183698]

备注/Memo

第一作者简介：秦佳乐（1999-），女，硕士研究生，研究方向为药用植物种质创新。E-mail:1261739477@qq.com.责任作者：李保会（1967-），男，硕士，研究员，现主要从事功能性植物种质创新及开发利用等研究工作。E-mail:kjjl@hebau.edu.cn.基金项目：河北省重点研发计划资助项目(21326312D-9)；河北省现代农业产业体系建设专项资助项目(HBCT2024190207)；保定市科技局资助项目(2494N002)；河北省中央财政林草科技推广示范资助项目(SFQ［2023］005)。收稿日期：2024-11-03

更新日期/Last Update: 2025-06-20