KOU Wenhua,WANG Xia,JIA Wenze.Effects of Exogenous 2,4-Epibrassinolide on Physiological and Biochemical Indexes in Rose Seedlings Under Low Temperature Stress[J].Northern Horticulture,2025,(9):87-94.[doi:10.11937/bfyy.20244095]
外施2,4-表油菜素内酯对低温胁迫月季苗生理生化指标的影响
- Title:
- Effects of Exogenous 2,4-Epibrassinolide on Physiological and Biochemical Indexes in Rose Seedlings Under Low Temperature Stress
- 文章编号:
- 1001-0009(2025)09-0087-08
- Keywords:
- rose; low temperature stress; 2; 4-epibrassionolide; physiological and biochemical indicators; membership function
- 分类号:
- S 685.12
- 文献标志码:
- A
- 摘要:
- 以月季品种‘Angela’为试验材料，采用生理生化指标测定和隶属函数分析的方法，研究低温胁迫下外施不同浓度的2,4-表油菜素内酯（EBR）对月季生理生化指标的影响，以期为月季在温度较低地区的种植和推广提供参考依据。结果表明：低温胁迫下，外施EBR可以明显提高月季的PSⅡ最大光化学量子产量（Fv/Fm）值和相对含水量（RWC）的下降速度，缓解相对电导率（REC）和丙二醛（MDA）含量的增加速度。同时，脯氨酸（Pro）含量、可溶性糖（SS）含量和可溶性蛋白质（SP）含量的增长速度有所缓解，且超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性和过氧化氢酶（CAT）活性的增长幅度也明显降低。综上所述，外施EBR可以明显提高月季的抗寒性，进一步通过隶属函数分析的方法发现外施0.4 mg·L-1EBR的效果最佳。
- Abstract:
- Taking the rose cultivar‘Angela’ as the test materials，physiological and biochemical indicators determination and membership function analysis method were used,the effects of different concentrations of 2,4-epibrassionolide (EBR) on the physiological and biochemical indicators of rose under low temperature stress were studied,in order to provide a reference for the planting and promotion in the lower temperature areas of rose seedlings.The results showed that spraying EBR under low temperature stress could significantly increase the maximal quantum yield of PSⅡ (Fv/Fm) value and the decrease rate of relative water content (RWC)，relief the rate of increase in relative conductivity (REC) and malondialdehyde (MDA) content in rose seedling leaves.At the same time，the growth rate of proline (Pro) content，soluble sugar (SS) content and soluble protein (SP) content were alleviated.In addition，the increase rate of superoxide dismutase (SOD) activity，peroxidase (POD) activity，and catalase (CAT) activity were also significantly reduced.In conclusion，the external application of EBR could significantly improve the cold resistance of rose seeding，and it was further found that the best effect of 0.4 mg·L-1EBR was applied by membership function analysis.
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备注/Memo
第一作者简介：寇文华（1986-），女，硕士，副教授，现主要从事植物生态环境及园林植物应用等研究工作。E-mail:kouwenhuakz@163.com.基金项目：国家自然科学基金资助项目（32001165）；重庆市高等教育教学改革研究重点资助项目（232147）。收稿日期：2024-10-17