LIU Songhu,LIANG Zhihao,LI Yihang,et al.Effects of Exogenous Melatonin Treatment Period on Growth of Cucumber Seedlings Under High Temperature Stress[J].Northern Horticulture,2025,(3):41-47.[doi:10.11937/bfyy.20243150]
外源褪黑素处理时期对高温胁迫下黄瓜幼苗生长的影响
- Title:
- Effects of Exogenous Melatonin Treatment Period on Growth of Cucumber Seedlings Under High Temperature Stress
- 文章编号:
- 1001-0009(2025)03-0041-07
- Keywords:
- cucumber; high temperaturestress; treatment period; melatonin; seedling growth
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以黄瓜品种“口口萃”为试材，采用100 mol·L-1的外源褪黑素（MT）对高温胁迫下的黄瓜幼苗进行叶面喷施，研究了外源MT不同处理时期对黄瓜幼苗表型和生理生化指标的影响，以期为解决黄瓜幼苗生长过程中遇到的高温胁迫问题提供参考依据。结果表明：在高温胁迫第1天对黄瓜幼苗进行MT处理能够显著促进幼苗株高、叶长、叶宽以及茎粗的生长；且能够显著增加黄瓜幼苗叶片的叶绿素含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性以及幼苗根系活力，降低丙二醛（MDA）的积累以及脯氨酸含量。综合分析表明，在高温胁迫第1天对幼苗进行MT处理可有效缓解高温胁迫所造成的伤害，提高植株的耐热性，促进黄瓜幼苗生长，为高温胁迫下喷施外源MT处理黄瓜幼苗的适宜时期。
- Abstract:
- Taking the cucumber variety ‘Koukoucui’ as the test material，100 μmol·L-1 exogenous melatonin (MT) was sprayed on the leaves of cucumber seedlings under high temperature stress.The effects of different treatment periods of exogenous MT on the phenotype and physiological and biochemical indicators of cucumber seedlings were studied，in order to provide reference for solving the problem of high temperature stress encountered during the growth process of cucumber seedlings.The results showed that MT treatment on cucumber seedlings at the 1st day of high temperature stress could significantly promote the growth of seedling height，leaf length，leaf width and stem diameter.It could significantly increase chlorophyll content，superoxide dismutase (SOD) activity，peroxidase (POD) activity and root activity of cucumber seedlings，and reduce malondialdehyde (MDA) accumulation and proline content.The comprehensive analysis showed that MT treatment on the 1st day of high temperature stress could effectively alleviate the damage caused by high temperature stress，improve the heat resistance of plants and promote the growth of cucumber seedlings，which was the appropriate period for exogenous MT treatment of cucumber seedlings under high temperature stress.
参考文献/References:
［1］田婧，郭世荣．黄瓜的高温胁迫伤害及其耐热性研究进展［J］．中国蔬菜，2012(18)：43-52.［2］张丰寅,杨再强,杨立,等.高温高湿对苗期黄瓜叶片光合特性和保护酶活性的影响［J］.北方园艺,2022(16):1-8.［3］徐向东，孙艳，孙波，等．高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响［J］．应用生态学报，2010，21(5)：1295-1300.［4］付丽军，李聪晓，苏胜宇，等．黄瓜苗期耐热种质筛选与耐热性评价体系构建［J］．植物生理学报，2020，56(7)：1593-1604.［5］宋聪慧，郭水欢，史小强，等．褪黑素调控果蔬采后保鲜研究进展［J］．食品科学，2023，44(3)：228-236.［6］卞凤娥，孙永江，牛彦杰，等．高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响［J］．植物生理学报，2017，53(2)：257-263.［7］SAQIB M，SHAHZAD U，ZULFIQAR F，et al．Exogenous melatonin alleviates cadmium-induced inhibition of growth and photosynthesis through upregulating antioxidant defense system in strawberry［J］．South African Journal of Botany，2023，157：10-18．［8］NAWAZ MA，JIAO Y，CHEN C，et al．Melatonin pretreatment improves vanadium stress tolerance of watermelon seedlings by reducing vanadium concentration in the leaves and regulating melatonin biosynthesis and antioxidant-related gene expression［J］．Journal of Plant Physiology，2018，220：115-127.［9］银珊珊，周国彦，顾博文，等．褪黑素引发对干旱胁迫下黄瓜幼苗生理特性的影响［J］．中国农学通报，2022，38(19)：30-36.［10］徐向东．外源褪黑素对高温胁迫下黄瓜幼苗生理反应的影响［D］．杨凌：西北农林科技大学，2010.［11］WANG Y，GUN S，LI Y，et al．Meta-analysis of effects of melatonin treatment on plant drought stress alleviation［J］．Agriculture，2022，12(9)：1335.［12］赵小红.温度胁迫下外源MT和CaCl2对黄瓜生长的影响［D］.重庆：西南大学，2018.［13］徐向东，孙艳，郭晓芹，等．高温胁迫下外源褪黑素对黄瓜幼苗光合作用及叶绿素荧光的影响［J］．核农学报，2011，25(1)：179-184.［14］赵娜，孙艳，王德玉，等．外源褪黑素对高温胁迫条件下黄瓜幼苗氮代谢的影响［J］．植物生理学报，2012，48(6)：557-564.［15］王博伟，陈艳丽，朱国鹏，等．叶面喷施褪黑素对海南高温季节水培叶用莴苣生长生理的影响［J］．中国蔬菜，2022(11)：80-85.［16］徐晨潇，张晓宇，刘超越，等．外源褪黑素与钙离子互作对高温胁迫下黄瓜幼苗过氧化伤害的缓解效应［J］．应用生态学报，2022，33(10)：2725-2735.［17］吴雪霞，张圣美，张爱冬，等．外源褪黑素对高温胁迫下茄子幼苗光合和生理特性的影响［J］．植物生理学报，2019，55(1)：49-60.［18］刘忠国．高温胁迫对黄瓜幼苗耐热性的研究［D］．泰安：山东农业大学，2010.［19］黄娟．高温胁迫下外源GABA对黄瓜幼苗生长的影响［J］．长江蔬菜，2016(8)：73-78.
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备注/Memo
第一作者简介：刘松虎（1977-），男，硕士，副教授，现主要从事蔬菜栽培生理与种质创新等研究工作。E-mail：shliu2012@126.com.责任作者：朱庆松（1976-），男，硕士，副教授，现主要从事设施作物栽培与遗传改良等研究工作。E-mail:zqs43@126.com.基金项目：河南省科技攻关资助项目（222102110443）；河南省高等学校重点科研资助项目（21B210009）。收稿日期：2024-08-02