WANG Degang,TANG Yuwei,YANG Mengyu,et al.Genetic Diversity and SSR Fingerprint Analysis of Shushanggan Apricot[J].Northern Horticulture,2025,(8):1-7.[doi:10.11937/bfyy.20242911]
树上干杏遗传多样性与SSR指纹图谱分析
- Title:
- Genetic Diversity and SSR Fingerprint Analysis of Shushanggan Apricot
- 文章编号:
- 1001-0009(2025)08-0001-07
- Keywords:
- Ili of Xinjiang; Shushanggan apricots; SSR molecular marker; DNA fingerprint; genetic diversity
- 分类号:
- S 662.6
- 文献标志码:
- A
- 摘要:
- 以新疆伊犁河谷61团及其他区域共11个采样点的树上干杏为试材，采用分子标记的鉴定方法，研究了树上干杏DNA水平上的遗传多样性，并构建DNA指纹图谱，以期为新疆树上干杏种质资源的保存与应用提供参考依据。结果表明：群体a的遗传多样性最高，其次是k、d群体，遗传多样性最小的为e群体（I=0.416）。AMOVA分子方差分析表明，居群内的变异是杏树群体遗传变异的主要来源，群体之间存在中等程度的遗传分化(Fst=0.13)。贝叶斯聚类分析、主坐标轴分析均支持11个群体可分为4个亚群，通过邻接聚类(neighbor-joining，NJ)分析可得杏树群体分为4类，其中e、c群体分别聚为一类，a、d、k群体聚为一类，b、f、g、h、j、y群体聚为一类，杏树不同群体中存在着一定的基因渗透。
- Abstract:
- The Shushanggan apricots from 11 sampling sites in the 61st Regiment and other main production areas of Yili Valley，Xinjiang，China were used as experimental materials.The genetic diversity of Shushanggan apricots at the DNA level was studied by molecular marker identification method，and the DNA fingerprints were constructed，in order to provide reference for the conservation and application of Xinjiang Shushanggan apricots germplasm resources.The results showed that population a had the highest genetic diversity,next were the k and d groups，the least was that of population e (I=0.416).AMOVA molecular variance indicated that intrapopulation variation was the main source of genetic variation in apricot population,there was moderate genetic differentiation among populations (Fst=0.13).Bayesian cluster analysis,Principal axis analysis all supported 11 populations that could be divided into 4 subgroups.Through neighbor-joining analysis,the apricot population could be divided into four categories.Among them，groups e and c were clustered into one category，groups a,d and k were clustered into one category,groups b,f,g,h,j,y were clustered into one category.There was some gene penetration in different populations of apricots.
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备注/Memo
第一作者简介：王德钢（1994-），男，硕士，助理研究员，现主要从事果树种质资源利用及栽培管理等研究工作。E-mail:1462127987@qq.com.责任作者：何梦雅（1989-），女，硕士，林业工程师，现主要从事林业与林果业等研究工作。E-mail:359103844@qq.com.基金项目：新疆生产建设兵团第四师可克达拉市科技计划资助项目（2022NY15）。收稿日期：2024-07-12