ZHANG Yanping,YE Chunlei,QI Yanni,et al.Salt-tolerant Gene Mapping of Flax Using BSA-Seq Analysis[J].Northern Horticulture,2025,(3):27-34.[doi:10.11937/bfyy.20242807]
基于BSA-Seq定位胡麻耐盐相关基因位点
- Title:
- Salt-tolerant Gene Mapping of Flax Using BSA-Seq Analysis
- 文章编号:
- 1001-0009(2025)03-0027-08
- Keywords:
- flax; BSA-Seq; salt resistance gene
- 分类号:
- S 853.75
- 文献标志码:
- A
- 摘要:
- 以胡麻耐盐材料STS和盐敏感材料DYMS及其杂交后代RIL群体为试材，采用BSA-Seq方法，研究筛选与胡麻抗盐相关的QTLs，以期为胡麻创新培育耐盐种质和分子标记辅助育种提供参考依据。结果表明：经过一系列分析筛选出14个与胡麻耐盐相关的SNP位点，其中第2号染色体上分布了8个，第3条染色体上分布了2个，第5、6、9号和13号染色体上各分布1个。通过实时荧光定量PCR过表达验证，获得一个候选基因Lus_GLEAN_10024365，其在第2号染色体上，scaffold208号位点5 913，基因长度4 981 bp。通过注释发现Lus_GLEAN_10024365与胞间连丝胼胝质（β-1,3-葡萄糖聚合物）结合蛋白合成相关，而胼胝质在盐胁迫过程中会有沉积。有关该基因与胼胝质合成与代谢的相关性尚需进一步深入研究。
- Abstract:
- The salt-tolerant STS and salt-sensitive DYMS and their hybrid offspring RIL populations of flax were used as the test materials,the BSA-Seq method was used，QTLs related to flax salt resistance were studied and screened，in order to provide reference for innovative cultivation of salt-tolerant germplasm and molecular marker-assisted breeding of flax.The results showed that 14 SNPS related to flax salt tolerance were selected after a series of analyses.8 SNP loci were screened on chromosome 2，2 SNP loci were screened on chromosome 3，and 1 SNP loci was screened on chromosome 5，6，9 and 13,respectively.Overexpression verified by real-time fluorescence quantitative PCR showed that the Lus_GLEAN_10024365 gene was a salt-tolerance candidate gene.It was on chromosome 2，which was scaffold208 on loci 5 913 with a length of 4 981 bp.It was found that Lus_GLEAN_10024365 was related to the synthesis of plasmodesmata callose (β-1，3-glucose polymer) binding protein which was deposited during salt stress.The correlation between this gene and the synthesis and metabolism of callose will be further investigated.
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备注/Memo
第一作者简介：张艳萍（1978-），女，本科，副研究员，现主要从事胡麻抗盐等研究工作。E-mail：64929217@qq.com.责任作者：叶春雷（1980-），男，本科，副研究员，现主要从事胡麻育种及示范推广等研究工作。E-mail：362240481@qq.com.基金项目：国家自然科学基金资助项目（32360502）；国家现代农业产业技术体系资助项目（CARS-14-2-23）；甘肃省重点研发计划资助项目（24YFWA002）；兰州市科技计划资助项目（2023-3-37）；兰州市安宁区科技计划资助项目（2023-JB-8）；甘肃省农科院院列科技创新专项资助项目（2021GAAS18）。收稿日期：2024-07-04