TANG Yanping,FAN Shichang,LI Mingjiao,et al.Isolation and Identification of Mycoparasites of Chinese Rose Powdery Mildew[J].Northern Horticulture,2025,(1):89-96.[doi:10.11937/bfyy.20242454]
月季白粉病菌重寄生菌的分离鉴定
- Title:
- Isolation and Identification of Mycoparasites of Chinese Rose Powdery Mildew
- 文章编号:
- 1001-0009(2025)01-0089-08
- 分类号:
- S 685.12
- 文献标志码:
- A
- 摘要:
- 以患有白粉病的月季叶片为试材，采用组织分离法分离重寄生真菌，并依据回接验证确定其寄生能力，结合形态学特征及rDNA-ITS序列鉴定重寄生菌的种类，以期为该病的防控提供参考依据。结果表明：从具有典型症状的病叶上分离得到13株重寄生真菌，寄生能力强的代表性菌株KMR3和KMR12寄生闭囊壳后，白粉孢子胞壁破碎，内含物释出。菌株KMR3菌落初期为白色，后期逐渐发展为棕褐色菌落，分生孢子为棕褐色，卵形、长棒状、椭圆形或倒梨形，ITS 序列与Alternaria alternata（MT482506.1）、Alternaria arborescens（PP097783.1）等均有 99%的相似性，在系统进化树中，菌株KMR3的序列和Alternaria alternata（MT482506.1）、Alternaria arborescens（PP097783.1）的距离最近；菌株KMR12菌落初期为白色，后期逐渐发展为棕褐色或浅黄色菌落，分生孢子呈棕褐色或黑褐色，椭圆形、卵形、棒状或倒梨形，ITS 序列与Alternaria angusstiovoidea（MK910070.1）、Alternaria longipes（MK675101.1）等均有 100%的相似性，在系统进化树中，菌株KMR12的序列和Alternaria angusstiovoidea（MK910070.1）、Alternaria longipes（MK675101.1）的距离最近。利用形态特征和 ITS 序列分析，鉴定出菌株 KMR3 和 KMR12为链格孢属。
- Abstract:
- Taking the leaves of Chinese rose powdery mildew as the test materials，the mycoparasite were isolated by the tissue separation method，and their parasitic ability was determined according to the reconnection test.Combined with morphological characteristics and rDNA-ITS sequence determination，the species of mycoparasite were identified,in order to provide a reference for disease prevention and control.The results showed that 13 strains of mycoparasite were isolated from the diseased leaves with typical symptoms.After the representative strains KMR3 and KMR12 with strong parasitic ability parasitised the capsule shell，the cell wall of powdery mildew spores was broken and the contents were released.The colony of strain KMR3 was white at the beginning，and then gradually developed into brown colony.The conidia were brown，oval，long rod，oval or inverted pear-shaped.ITS sequence was 99% similar to Alternaria alternata (MT482506.1) and Alternaria arborescens (PP 097783.1).In the phylogenetic tree，the sequence of strain KMR3 was the closest to Alternaria alternata (MT482506.1) and Alternaria arborescens (PP 097783.1).The colony of strain KMR12 was white at the initial stage，and gradually developed into brown or light yellow colony at the later stage.The conidia were brown or dark brown，oval，rod-shaped or inverted pear-shaped，and ITS sequence was 100% similar to those of Alternaria angustifoidea (MK 910070.1) and Alternaria longipes(MK675101.1).In the phylogenetic tree，the sequence of strain KMR12 was the closest to that of Alternaria angustifoidea (MK 910070.1) and Alternaria longipes(MK675101.1).Using morphological characteristics and ITS sequence analysis，strains KMR3 and KMR12 were identified as Alternaria.
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备注/Memo
第一作者简介：汤燕萍（1998-），女，硕士研究生，研究方向为资源微生物的开发与利用。E-mail:typ1872739499@163.com.责任作者：李靖（1978-），女，博士，教授，现主要从事微生物生理生化等研究工作。E-mail：lijingcas@163.com.基金项目：云南省农业联合重点资助项目（202301BD070001-159）；中央引导地方科技发展专项资金资助项目(219001)。收稿日期：2024-06-11