LYU Yunhao,JIANG Ying,YU Shuli,et al.Effects of Botrytis cinerea Infection on Postharvest Quality and Active Oxygen Metabolism of ‘Munage’ Grape[J].Northern Horticulture,2025,(1):51-59.[doi:10.11937/bfyy.20242327]
灰霉菌侵染对“木纳格”葡萄采后品质和活性氧代谢的影响
- Title:
- Effects of Botrytis cinerea Infection on Postharvest Quality and Active Oxygen Metabolism of ‘Munage’ Grape
- 文章编号:
- 1001-0009(2025)01-0051-09
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 以“木纳格”葡萄为试材，分别用灰霉菌孢子悬浮液和无菌水处理采后葡萄，并在（25.0±0.5）℃、相对湿度85%~90%下贮藏，每隔3 d测定“木纳格”葡萄的生理品质和活性氧代谢相关指标并分析其相关性，研究灰霉菌侵染对“木纳格”葡萄采后品质及活性氧代谢的影响，以期为“木纳格”葡萄采后贮藏保鲜及后期葡萄生物保鲜研提供参考依据。结果表明：灰霉菌侵染促使果实呼吸强度增强（13.11%），乙烯峰值（45.70 μL·kg-1·h-1）提前出现，加快了果实腐烂（51.51%）及硬度（4.15 N）的下降，总酚（0.39 mg·g-1）和总黄酮（5.08 mg·g-1）在贮藏末期含量均低于对照。灰霉菌侵染还破坏了葡萄活性氧动态平衡，导致果实中H2O2和O·2的过多产生，贮藏末期丙二醛含量（0.24 μmol·mg-1）显著提高。在侵染前中期，处理果实中的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）等抗氧化酶活性均高于对照果实。
- Abstract:
- ‘Munage’ grapes were used as the test material.Postharvest grapes were treated with Botrytis cinerea spore suspension and sterile water，respectively，and stored at (25±0.5)℃ and relative humidity of 85%-90%.The physiological quality and active oxygen metabolism of ‘Munage’ grapes were measured every 3 days，and the correlation was analyzed，the effects of Botrytis cinerea infection on postharvest quality and active oxygen metabolism of ‘Munage’ grape were studied，in order to provide reference for postharvest storage and preservation of ‘Munage’ grape，and to lay a theoretical foundation for the study of biological preservation of grape in the later period.The results showed that Botrytis cinerea infection increased the respiratory intensity of fruit (13.11%)，advanced the ethylene peak (45.70 μL·kg-1·h-1)，accelerated the decay of fruit (51.51%) and the decline of fruit firmness (4.15 N).The contents of total phenolics (0.39 mg·g-1) and total flavoids (5.08 mg·g-1) at the end of storage were lower than those of control.The infection of Botrytis cinerea also destroyed the dynamic balance of reactive oxygen species，resulting in excessive production of H2O2 and O·2 in grapes，and significantly increased the content of malondialdehyde (0.24 μmol·mg-1) at the end of storage.The activities of SOD，POD，CAT，APX and other antioxidant enzymes in treated fruits were higher than those in control fruits before and during the middle period of infection.
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备注/Memo
第一作者简介：吕云皓（1995-），男，博士研究生，研究方向为果蔬贮藏与加工。E-mail:1142114656@qq.com.责任作者：刘忆冬（1975-），女，博士，副教授，现主要从事果蔬加工与贮藏等研究工作。E-mail:393892096@qq.com.基金项目：国家自然科学基金资助项目(32160590)。收稿日期：2024-06-03