SHAN Qinli,YANG Chunmei,WU Lifang,et al.Genetic Expression of Double Haploid Inflorescence in Gerbera hybrida Based on Ovule Induction Cultivation[J].Northern Horticulture,2025,(1):1-7.[doi:10.11937/bfyy.20242240]
基于胚珠诱导培育的非洲菊双单倍体花序遗传表现
- Title:
- Genetic Expression of Double Haploid Inflorescence in Gerbera hybrida Based on Ovule Induction Cultivation
- 文章编号:
- 1001-0009(2025)01-0001-07
- Keywords:
- Gerbera hybrida; double haploid; breeding; heredity
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以非洲菊品种及其胚珠诱导培育的双单倍体为试验材料，采用田间完全随机排列方法，对开花植株的花色、花序类型和花心颜色进行观测，解析非洲菊的花序遗传表现，以期为非洲菊定向育种的亲本选择提供参考依据。结果表明：非洲菊的花色和花心表现为偏母性遗传趋势，其中红色品种的后代双单倍体植株花色和母本植株一致的概率达95% 以上，花心颜色与母本植株一致的概率达55% 以上；单瓣品种后代双单倍体植株的花序类型偏母性遗传倾向明显，和母本植株一致的概率达90%以上；重瓣和半重瓣品种后代双单倍体植株的花序类型未表现出明显的偏母性遗传倾向，和母本植株一致的概率不到35%。
- Abstract:
- Taking Gerbera hybrida varieties and their double haploids induced from ovules as the test materials,the field completely random arrangement method was used to observe the flower color，inflorescence type，and flower center color of flowering plants，and analyzed the genetic performance of Gerbera hybrida inflorescence，in order to provide reference for parental selection in directional breeding of Gerbera hybrida.The results showed that the color of the flower petal and flower center of Gerbera hybrida showed a tendency towards maternal inheritance，and the probability of over 95% for the offspring of red varieties with double haploid plants to have the same flower color as the maternal plant，and the probability of flower center color being consistent with the maternal plant was over 55%.The inflorescence types of double haploid plants in the offspring of single petal varieties had a significant tendency towards maternal inheritance，and the probability of being consistent with the maternal plants was more than 90%.The inflorescence types of double haploid plants derived from double petal varieties and semidouble petal varieties did not show a significant maternal tendency，and the probability of being consistent with the maternal plants was less than 35%.
参考文献/References:
［1］LEFFRING L．Flower production in Gerbera:I．Correlations between shoot,leaf and flower formation in seedlings［J］．Scientia Horticulturae,1973,1(3):221-229.［2］LI F,LI S,SHAN Q．The effect of temperature on plant growth in four Gerbera hybrida cultivars［J］．HortScience,2019,54(7):1164-1167.［3］SINGH S，DHYANID，KARTHIGEYAN S.Characterization of variability in F1 progeny of inter-varietal cross of gerbera (Gerbera jamesonii Bolus ex.Hooker F)［J］.Journal of Ornamental Horticulture，2009，12(4)：225-231.［4］李绅崇,蒋亚莲,曹桦．切花非洲菊品种自交亲和性研究［J］．现代园艺,2008(9):44-46.［5］单芹丽,王继华,李绅崇,等．秋水仙素对非洲菊单倍体加倍效果研究［J］．西南农业学报,2017,30(10):2230-2234.［6］GERMAN M A．Anther culture for haploid and doubled haploid production［J］．Plant Cell,Tissue and Organ Culture (PCTOC),2011,104(3):283-300.［7］OLLITRAULT P,DAMBIER D,LURO F,et al．Ploidy manipulation for breeding seedless triploid Citrus［J］．Plant Breeding Reviews,2008（30）:323-352.［8］LI S．Comparative transcriptome analysis of two Gerbera hybrida cultivars revealed cold tolerance mechanism［J］．Plant Omics,2018,11(1):10-19.［9］ZOU J,RAMAN H,GUO S,et al．Constructing a dense genetic linkage map and mapping QTL for the traits of flower development in Brassica carinata［J］．Theoretical and Applied Genetics,2014,127(7):1593-1605.［10］YE L,HUANG Y,DAI F,et al．Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L.)［J］．BMC Genomics,2015,16(1):449.［11］ALEZA P,JUREZ J,HERNNDEZ M,et al．Recovery and characterization of a Citrus clementina Hort．ex Tan．‘Clemenules’ haploid plant selected to establish the reference whole Citrus genome sequence［J］．BMC Plant Biology,2009（9）:110.［12］单芹丽,李绅崇,王继华,等．基因型和预处理对非洲菊胚珠离体培养的影响［J］．江西农业学报,2015,27(7):44-47.［13］单芹丽,王继华,吴丽芳,等．不同供体材料对非洲菊胚珠离体培养的影响［J］．植物生理学报,2015,51(7):1151-1156.［14］王丽花,瞿素萍,杨秀梅,等．非洲菊未授粉胚珠的离体诱导和植株再生［J］．植物生理学通讯,2007,43(6):1089-1092.［15］LI F,CHENG Y,ZHAO X,et al．Haploid induction via unpollinated ovule culture in Gerbera hybrida［J］．Scientific Reports,2020,10(1):1702.［16］单芹丽,李绅崇,吴丽芳,等．非洲菊单倍体植株生根研究［J］．江西农业学报,2019,31(11):29-33.［17］王丽花,杨秀梅,吴学尉,等．非洲菊大孢子再生植株倍性的快速鉴定方法［J］．西北农业学报,2013,22(1):155-161.［18］单芹丽,汪国鲜,吴旻,等．非洲菊单倍体移栽苗培育的关键技术［J］．热带生物学报,2020,11(3):341-346.［19］单芹丽,阮继伟,吴丽芳,等．非洲菊单倍体植株的生长特性初报［J］．江西农业学报,2016,28(12):13-17.［20］BURCHI G,PRISA D,BALLARIN A,et al．Improvement of flower color by means of leaf treatments in lily［J］．Scientia Horticulturae,2010,125(3):456-460.［21］YAMAGISHI M,IHARA H,ARAKAWA K,et al．The origin of the LhMYB12 gene,which regulates anthocyanin pigmentation of tepals,in oriental and Asiatic hybrid lilies (Lilium spp．)［J］．Scientia Horticulturae,2014,174:119-125.［22］李绅崇，李淑斌，蒋亚莲，等.非洲菊品种间杂交主要观赏性状在Fl代的遗传表现［J］.云南农业大学学报,2007，22(2)：197-201.［23］董雪娜，陈希，蒋甲福，等.非洲菊F1代观赏性状的遗传表现［J］.南京农业大学学报，2015，38(2)：226-232.
相似文献/References:
[1]尹立荣,管长志,陈磊,等.胡萝卜游离小孢子培养与植株再生技术研究[J].北方园艺,2012,36(15):126.
YIN Li-rong,GUAN Chang-zhi,CHEN Lei,et al.Study on Isolated Microspore Culture and Plant Regeneration of Carrot[J].Northern Horticulture,2012,36(1):126.
[2]乔永旭,张永平,张红心,等.非洲菊再生体系的建立[J].北方园艺,2012,36(18):133.
QIAO Yong-xu,ZHANG Yong-ping,ZHANG Hong-xin,et al.Establishment of Regeneration System of Gerbera[J].Northern Horticulture,2012,36(1):133.
[3]李飞鹏,曹荣根,舒洋,等.四种红色系非洲菊品种特性的比较[J].北方园艺,2012,36(20):55.
LI Fei-peng,CAO Rong-gen,SHU Yang,et al.Comparison of Characteristics of Four Red Cultivars of Gerbera jamesonii[J].Northern Horticulture,2012,36(1):55.
[4]刘 旭,刘 博,吕春华,等.不同激素处理对非洲菊开花的影响[J].北方园艺,2012,36(24):83.
LIU Xu,LIU Bo,LV Chun-hua,et al.Effect of Different Hormone Treatments on Flowering of Gerbera[J].Northern Horticulture,2012,36(1):83.
[5]王洁琼,彭绍峰,周子发,等.非洲菊试管苗生根培养方法的优化[J].北方园艺,2012,36(13):140.
WANG Jie-qiong,PENG Shao-feng,ZHOU Zi-fa,et al.The Optimization of Gerbera Plantlets Rooting Methods[J].Northern Horticulture,2012,36(1):140.
[6]徐玲丽,张鸿翎,韩玲玲,等.鲜切花家用保鲜剂的配方研究[J].北方园艺,2012,36(19):165.
XU Ling-li,ZHANG Hong-ling,HAN Ling-ling,et al.Study on the Formula of the Preservation of Cut Flower Freshness[J].Northern Horticulture,2012,36(1):165.
[7]冯 会.不同温度下GA 3 对非洲菊采后生理效应的影响[J].北方园艺,2010,34(05):0.[doi:10.11937/bfyy.201005065]
FENG Hui,PAN Dong -ming.Influence of Post-harvest Physiology of Gerbera in Different T emperature Conditionswith Different Concentrations of GA 3 in Preservation[J].Northern Horticulture,2010,34(1):0.[doi:10.11937/bfyy.201005065]
[8]桂 敏,陈 敏,龙 江,等.非洲菊切花品种引种试种研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010033]
GUI Min,CHEN Min,LONG Jiang,et al.Research of Introduction and T rial of Cultivars for Gerbera jamesonii[J].Northern Horticulture,2010,34(1):0.[doi:10.11937/bfyy.2010010033]
[9]蔡 小 东,胡 文 静.非洲菊愈伤组织和不定芽的诱导[J].北方园艺,2010,34(21):0.[doi:10.11937/bfyy.201021063]
CAI Xiao -dong,HU Wen -jing.Callusand Shoots Induction of Gerbera jamesoni[J].Northern Horticulture,2010,34(1):0.[doi:10.11937/bfyy.201021063]
[10]吴 庆 丽.温室非洲菊组培苗灰霉病发生规律与防治技术[J].北方园艺,2010,34(12):0.[doi:10.11937/bfyy.2010012069]
WU Qing -li.Incidence and Control of Gerbera in vitro Plantlet of Botrytiscinerea in Greenhouse[J].Northern Horticulture,2010,34(1):0.[doi:10.11937/bfyy.2010012069]
备注/Memo
第一作者简介：单芹丽（1973-），女，硕士，研究员，现主要从事非洲菊种质创新、种苗繁育及栽培等研究工作。E-mail:1249087008@qq.com.责任作者：李绅崇（1975-），男，博士，研究员，现主要从事观赏园艺育种及绿色生产等研究工作。E-mail:693243551@qq.com.基金项目：云南省重大科技专项计划资助项目（202102AE090052）；兴滇英才支持计划和云南省技术创新人才培养对象资助项目（2018HB117）。收稿日期：2024-05-27