HUANG Jing,HE Yaowei,LI Peng,et al.Analysis of the Correlation Between Mycelial Characteristics and Extracellular Enzyme Activity in Subsequent Cultivation of Agrocybe aegerita[J].Northern Horticulture,2025,(2):50-58.[doi:10.11937/bfyy.20242054]
菌丝特性及胞外酶活性对茶树菇继代培养菌丝的相关性分析
- Title:
- Analysis of the Correlation Between Mycelial Characteristics and Extracellular Enzyme Activity in Subsequent Cultivation of Agrocybe aegerita
- 文章编号:
- 1001-0009(2025)02-0050-09
- Keywords:
- A.aegerita; subculture; mycelial characteristics; extracellular enzyme system; correlation
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以茶树菇CS8、CS15、CS16为试材，采用继代培养方法，研究了不同继代次数对茶树菇菌丝的形态、生长速度、胞外酶活性、菌丝吃料快慢的影响，以期为后续茶树菇的生产提供参考依据。结果表明：随着继代次数的增加，茶树菇菌丝的生长速度逐渐降低、菌丝密度逐渐变稀疏、菌丝活力在F5开始逐渐降低；淀粉酶活性随着继代次数的增加没有显著相关性；纤维素酶和蛋白酶在F1就开始下降，木聚糖酶在F3或F5开始下降，漆酶在F5或F7开始下降；对各指标进行相关性分析，发现菌丝吃料快慢受母种菌丝生长速度、漆酶和纤维素酶活性影响，木聚糖酶与菌丝吃料速度呈负相关，蛋白酶和淀粉酶与茶树菇菌丝吃料无显著相关性或相关性不显著。因此，为确保菌种的优良性状及活性，继代次数应严格控制在F5以内，且选择漆酶和纤维素酶活性高的菌株。
- Abstract:
- Taking Agrocybe aegerita CS8，CS15 and CS16 as the experimental materials，the effects of different subculture times on mycelia morphology，growth rate，extracellular enzyme activity and mycelia feeding speed of Agrocybe aegerita were studied，in order to provide reference for the subsequent production of Agrocybe aegerita.The results showed that with the increase of subgenerations，the growth rate of mycelia gradually decreased，the density of mycelia gradually became sparse，and the activity of mycelia gradually decreased in F5.Amylase activity had no significant correlation with the increase of subgenerations.Cellulase and protease began to decline in the F1，xylanase began to decline in F3 or F5，laccase began to decline in F5 or F7.Correlation analysis of each index showed that mycelia feeding speed was affected by parent mycelia growth rate，laccase and cellulase activities，xylanase was negatively correlated with mycelia feeding speed，protease and amylase were not significantly correlated with mycelia feeding.Therefore，in order to ensure the good properties and activities of the strains，the number of subgenerations should be strictly controlled within F5，and strains with high laccase and cellulase activities should be selected.
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备注/Memo
第一作者简介：黄静（1993-），女，硕士，助理研究员，现主要从事食用菌类种质资源创新与技术栽培推广等工作。Email：1250886036@qq.com.责任作者：向准（1976-），男，苗族，硕士，副研究员，现主要从事食用菌品种选育与栽培等研究工作。Email：564375391@qq.com.基金项目：贵州省科技计划资助项目（黔科合中引地［2024］026，黔科合重大专项字［2019］30075号，黔科合成果［2020］1Z003号，黔科合支撑［2021］一般195）；贵州省食用菌现代农业产业技术体系建设资助项目（GZCYTX2024）；2023年省级十二个农业特色优势产业专班资助项目（GZTSCYZBSYZ 2023001）。收稿日期：2024-02-08