LIU Zhijin,SHAN Lulu,ZHANG Wenqi,et al.Soil Fungal Diversity in Different Age Walnut Orchards in Southern Xinjiang[J].Northern Horticulture,2024,(24):61-68.[doi:10.11937/bfyy.20241865]
南疆不同树龄核桃园土壤真菌多样性
- Title:
- Soil Fungal Diversity in Different Age Walnut Orchards in Southern Xinjiang
- 文章编号:
- 1001-0009(2024)24-0061-08
- Keywords:
- walnut; soil fungi; fungal diversity; high-throughput sequencing
- 分类号:
- S 664.1
- 文献标志码:
- A
- 摘要:
- 以南疆不同树龄核桃园土壤为试材,采用Illumina Mieq高通量测序方法,研究了不同树龄土壤真菌多样性,以期为生物菌肥及生防菌剂的开发与应用提供参考依据。结果表明:不同树龄核桃园土壤真菌群落结构存在明显差异,但不同树龄土壤真菌优势类群均为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)以及接合菌门(Zygomycota)。且随着年份的增长土壤真菌丰富度先增加后降低,而多样性随年份增加逐年升高。在门、纲、目、科和属分类水平上,丰度一直增加的土壤真菌类群分别为子囊菌门(Ascomycota)、酵母菌纲(Saccharomycetes)、散囊菌纲(Eurotiomycetes)、酵母菌目(Saccharomycetales)、小鬼伞科(Psathyrellaceae)、柯达酵母属(Kodamaea)、假鬼伞属(Coprinellus)。该研究揭示了南疆不同树龄核桃园土壤真菌群落的多样性,探明了不同树龄核桃园土壤真菌群落结构的变化规律,为开发利用土壤真菌资源和深入研究土壤真菌多样性与果树营养水平之间的联系打下基础。
- Abstract:
- Taking the soil of different walnut orchards in southern Xinjiang as the test material,Illumina Mieq high-throughput sequencing method was used to study the fungal diversity in soil of different ages,in order to provide reference for the development and application of bio-fertilizers and biocontrol agents.The results showed that there were significant differences in soil fungal community structure among different tree ages,but the dominant groups of soil fungi in different tree ages were Ascomycota,Basidiomycota and Zygomycota.With the increase of years,the richness of soil fungi first increased and then decreased,while the diversity increased with the increase of years.At the level of phyla,class,order,family,and genus classification,the soil fungi that had been increasing in abundance were Ascomycota,Saccharomycetes,Eurotiomycetes,Saccharomycetales,and Psathyrellaceae,Kodamaea,Coprinellus.The diversity of soil fungal community in walnut orchard of different ages and the changes of soil fungal community structure in walnut orchard of different ages were clarified,which laid a foundation for the development and utilization of soil fungal resources and further research on the relationship between soil fungal diversity and fruit tree nutrition level.
参考文献/References:
[1]沈旭,郑洁,侯如燕.核桃的营养价值及功效[J].食品安全导刊,2015(23):76-78.[2]傅本重,邹路路,朱洁倩,等.中国核桃生产现状与发展思路[J].江苏农业科学,2018,46(18):5-8.[3]BAI L,CUI J,JIE W,et al.Analysis of the community compositions of rhizosphere fungi in soybeans continuous cropping fields[J].Microbiological Research,2015,180:49-56.[4]SHEN Q,YANG J,SU D,et al.Comparative analysis of fungal diversity in rhizospheric soil from wild and reintroduced Magnolia sinica estimated via high-throughput sequencing[J].Plants,2020,9(5):600.[5]管冠,何天养,朱婧,等.柑橘黄龙病对赣南脐橙果园土壤生物学性质的影响[J].江苏农业科学,2017,45(20):162-164.[6]任利利,王焱,马风林,等.上海地区果树根癌病发生与土壤环境的关系[J].生态环境,2007,16(2):498-502.[7]吴斌,姜珊珊,张眉,等.小麦黄花叶病毒病不同发生度土壤的微生物多样性研究[J].山东农业科学,2017,49(12):44-49.[8]孙广宇,卫小勇,孙悦,等.苹果树腐烂病发生与叶片营养成分的关系[J].西北农林科技大学学报(自然科学版),2014,42(7):107-112.[19]CALLAHAN B J,MCMURDIE P J,ROSEN M J,et al.DADA2:High-resolution sample inference from Illumina amplicon data[J].Nature Methods,2016,13(7):581-583.[10]孙杨,王璐,赵璐,等.复合微生物菌肥对苹果再植病害调控及对根围土壤真菌群落结构的影响[J].植物病理学报,2022,52(2):256-268.[11]慕飞飞.西安市秋冬季微生物气溶胶的垂直分布特征研究[D].西安:长安大学,2020.[12]ROGNES T,FLOURI T,NICHOLS B,et al.VSEARCH:A versatile open source tool for metagenomics[J].PeerJ,2016,4:2584.[13]李欣蔚.基于高通量测序探究东北粳稻储藏期间真菌群落的演替[J].现代食品科技,2022,38(7):98-106.[14]ZAJC J,KOGEJ ZWITTER Z,FIER S,et al.Highly specific qPCR and amplicon sequencing method for detection of quarantine citrus pathogen Phyllosticta citricarpa applicable for air samples[J].Plant Pathology,2023,72(3):548-563.[15]KIM M.Invited review-assessment of the gastrointestinal microbiota using 16S ribosomal RNA gene amplicon sequencing in ruminant nutrition[J].Animal Bioscience,2023,36(2):364-373.[16]徐红梅,倪方方,宋腾蛟,等.高通量测序揭示杭白芍根际真菌群落年际变化[J].浙江中医药大学学报,2016,40(12):942-947.[17]李倩,杨水平,崔广林,等.不同种植年限条件下黄花蒿根际土壤微生物生物量、酶活性及真菌群落组成[J].草业学报,2017,26(1):34-42.[18]李越鲲,孙燕飞,雷勇辉,等.枸杞根际土壤真菌群落多样性的高通量测序[J].微生物学报,2017,57(7):1049-1059.[19]蔡丽琼,陈瑞,杨德强,等.基于高通量测序的天麻连作根际土壤真菌群落多样性分析[J].中国麻业科学,2022,44(6):321-330.
备注/Memo
第一作者简介:刘志金(1999-),男,硕士研究生,研究方向为植物病理学。E-mail:3115273782@qq.com.责任作者:陈小飞(1978-),男,硕士,教授,现主要从事作物病虫害绿色防控等研究工作。E-mail:cxfzky@126.com.基金项目:国家自然科学基金地区基金资助项目(31560007);国家林业科技成果推广资助项目([2019]55);新疆建设兵团“三区”人才支持计划科技人员专项计划资助项目(BT1720230048G)。收稿日期:2024-05-04