[1]王伟霞,童小嫣,陈源,等.海鲜菇采后贮藏过程的转录组分析及褐变基因的挖掘[J].北方园艺,2025,(1):21-28.[doi:10.11937/bfyy.20241678]
　WANG Weixia,TONG Xiaoyan,CHEN Yuan,et al.Transcriptome Analysis of Hypsizygus marmoreus of Post Harvest Storage Process and Exploration of Browning Genes[J].Northern Horticulture,2025,(1):21-28.[doi:10.11937/bfyy.20241678]
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海鲜菇采后贮藏过程的转录组分析及褐变基因的挖掘

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年1 页码: 21-28 栏目: 出版日期: 2025-01-15
Title:
Transcriptome Analysis of Hypsizygus marmoreus of Post Harvest Storage Process and Exploration of Browning Genes
文章编号:
1001-0009(2025)01-0021-08
作者:
王伟霞1; 2; 童小嫣1; 2; 陈源1; 2; 韩琦1; 2; 赵书光3; 李福后1; 2
（1.江苏海洋大学 海洋资源与环境重点实验室，海洋生物技术重点实验室，江苏 连云港 222005；2.江苏省海洋生物产业技术协同创新中心，江苏 连云港 222000；3.江苏省灌南县农业技术推广中心，江苏 灌南 222500）
Author(s):
WANG Weixia1; 2; TONG Xiaoyan1; 2; CHEN Yuan1; 2; HAN Qi1; 2; ZHAO Shuguang3; LI Fuhou1; 2
(1.Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University/Jiangsu Key Laboratory of Marine Biotechnology，Lianyungang，Jiangsu 222005；2.Co-Innovation Center of Jiangsu Marine Bio-Industry Technology，Lianyungang，Jiangsu 222000；3.Agricultural Technology Extension Center of Jiangsu Guannan County，Guannan，Jiangsu 222500)
关键词:
海鲜菇; 转录组测序; 漆酶; 过氧化物酶
Keywords:
Hypsizygus marmoreus; transcriptome sequencing; laccase; peroxidase
分类号:
S 759.81
DOI:
10.11937/bfyy.20241678
文献标志码:
A
摘要:
以海鲜菇子实体为试材，采用高通量测序技术，研究了海鲜菇子实体在贮藏过程中的转录组变化，以期为揭示子实体采后品质变化、抑制子实体褐变发生提供参考依据。结果表明：在4 ℃贮藏条件下，第10天与第0天相比，获得3 659个差异表达基因，其中上调基因1 707个，下调基因1 952个；1-MCP处理后第10天与第0天相比，获得4 142个差异表达基因，其中上调基因2 122个，下调基因2 020个。共筛选得到3个差异表达的漆酶基因和1个差异表达的过氧化物酶基因，分别是[STBX]LAC1、LAC2、LAC4和POD2[STBZ]，这些基因可能与子实体褐变发生具有密切联系。
Abstract:
Taking the fruit body of Hypsizygus marmoreus as the test meterial,the transcriptome changes of post harvest Hypsizygus marmoreus fruiting bodies during storage were studied by using high-throughput sequencing technology,in order to provide reference for revealing post harvest quality changes and inhibiting fruiting body browning.The results showed that 3 659 differentially expressed genes were obtained on the 10th day compared to the 0th day，included 1 707 upregulated genes and 1 952 downregulated genes under storage conditions of 4 ℃.Moreover，on the 10th day after 1-MCP treatment，compared to the 0th day，4 142 differentially expressed genes were obtained，included 2 122 upregulated genes and 2 020 downregulated genes.Three differentially expressed laccase genes and one differentially expressed peroxidase gene were screened，namely LAC1，LAC2，LAC4，and POD2.These genes might be closely related to the occurrence of browning in fruiting bodies.

参考文献/References:

［1］张婷,钱亮亮,王伟霞,等.斑玉蕈菌丝体富集钙的研究［J］.北方园艺,2022(4):104-109.［2］王红,刘俊杰,温浩,等.斑玉蕈种质资源评价及遗传多样性分析［J］.中国食用菌,2023,42(6):41-47.［3］张旭,潘先兴,田一鸣,等.食用菌褐变机理及抑制方法研究进展［J］.中国果菜,2022,42(5):38-44.［4］赵越,白晋豪,张素敏,等.热处理对海鲜菇质构特性和酶活性的影响［J］.农产品加工,2021(12):1-4,8.［5］DIAS C,FONSECA A M A,AMARO A L,et al.Natural-based antioxidant extracts as potential mitigators of fruit browning［J］.Antioxidants,2020,9(8):715.［6］SUI X,MENG Z,DONG T,et al.Enzymatic browning and polyphenol oxidase control strategies［J］.Current Opinion in Biotechnology,2023,81:102921.［7］OMS-OLIU G,ROJAS-GRA M A,GONZLEZ L A,et al.Recent approaches using chemical treatments to preserve quality of fresh-cut fruit:A review［J］.Postharvest Biology and Technology,2010,57(3):139-148.［8］NICOLAS J J,RICHARD-FORGET F C,GOUPY P M,et al.Enzymatic browning reactions in apple and apple products［J］.Critical Reviews in Food Science and Nutrition,1994,34(2):109-157.［9］鲍大鹏,谢宝贵.我国食用菌遗传学中一些值得关注的研究方向［J］.菌物学报,2020,39(6):971-976.［10］张建,黄天宇,鲍大鹏,等.利用转录组分析光照对香菇菌丝生长的影响［J］.食用菌学报,2023,30(3):1-10.［11］曹子健,胡宝,徐子昕,等.逆境胁迫下食用菌转录组学研究进展［J］.北方园艺,2022(6):131-136.［12］徐爱国,储婷,杨瑞恒,等.利用转录组挖掘羊肚菌继代菌丝退化相关基因的初步探讨［J］.食用菌学报,2023,30(1):10-16.［13］彭博,李炳娟,关文强,等.双孢蘑菇转录组测序及褐变相关基因的挖掘［J］.食品科学,2019,40(2):126-132.［14］杨和川,苏文英,谭一罗,等.玉木耳转录组测序及褐变相关基因的挖掘［J］.江西农业学报,2020,32(5):7-12.［15］储婷,尚俊军,管婉,等.香菇菌丝后熟转色的转录组分析［J］.菌物学报,2022,41(2):260-273.［16］贾乐,韩延超,房祥军,等.褪黑素处理对香菇采后品质及活性氧代谢的影响［J］.食品科学,2021,42(23):229-236.［17］董闪闪,范刘敏,李云菲,等.非热加工技术失活果蔬多酚氧化酶研究进展［J］.食品工业,2021,42(9):210-214.［18］吴光美,严俊杰,杨志云,等.与金针菇子实体发育有关的漆酶编码基因Fv-lac4的结构及表达差异分析［J］.基因组学与应用生物学,2014,33(6):1254-1260.［19］卓睿,马富英,周帅,等.糙皮侧耳生长发育过程中漆酶基因家族的表达研究［J］.菌物学报,2015,34(4):712-716.［20］朱海生,刘建汀,王彬,等.丝瓜过氧化物酶基因的克隆及其在果实褐变中的表达分析［J］.农业生物技术学报,2018,26(1):43-52.［21］张琪,额日赫木,王梦茹,等.真空浸渍辅助曲酸对鲜切马铃薯多酚氧化酶和过氧化物酶活性的抑制作用［J］.粮食与饲料工业,2020(1):8-15.［22］陈军,陈怀宇,陈洪彬,等.2,4,2′,4′-四羟基查尔酮协同抗坏血酸抑制双孢菇酶促褐变的研究［J］.食品科技,2023,48(10):63-71.［23］周陈力,郭婷,纪光燕,等.基于基因组和转录组挖掘暗褐网柄牛肝菌转录因子以及漆酶表达调控因子［J］.北方园艺,2023(16):108-116.［24］ZHANG J,CHEN H,CHEN M,et al.Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus［J］.Microbiological Research,2015,179:54-63.［25］JIN W,LI J,FENG H,et al.Importance of a laccase gene (Lcc1) in the development of Ganoderma tsugae［J］.International Journal of Molecular Sciences,2018,19(2):471.［26］李京赞,刘玉德,石文天,等.植物果蔬的褐变及抑制的研究［J］.包装与食品机械,2019,37(1):63-68.［27］李佳欣,冉海榕,傅玉凡,等.不同基因型甘薯块根褐变度的差异分析［J］.西南大学学报(自然科学版),2023,45(5):48-56.［28］代雨杉,马秀菊,毕秀芳.超声波结合热处理对莲藕多酚氧化酶和过氧化物酶钝化效果研究［J］.包装与食品机械,2021,39(5):20-26.［29］李彦虎,贠建民,毕阳,等.两种精油熏蒸处理对双孢蘑菇贮藏特性的影响［J］.食品与发酵工业,2019,45(11):191-198.［30］林青,周聪,李学进,等.马齿苋提取物对鲜切苹果褐变的影响［J］.食品科学技术学报,2021,39(6):108-114.

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备注/Memo

第一作者简介：王伟霞（1977-），女，硕士，讲师，现主要从事食用菌栽培及农林废弃物资源化利用等研究工作。E-mail:2003000023@jou.edu.cn.责任作者：李福后（1978-），男，博士，副教授，现主要从事食用菌栽培及农林废弃物资源化利用等研究工作。E-mail:lifuhou2002@163.com.基金项目：江苏海洋大学2023年大学生创新创业训练计划资助项目（SY202311641638004）。收稿日期：2024-04-18

更新日期/Last Update: 2025-01-16