YUAN Qingsong,GAO Yanping,GUO Lanping,et al.Effects of Fusarium Wilt on Soil Microbial Structure and Function of Pseudostellaria heterophylla[J].Northern Horticulture,2024,(23):73-82.[doi:10.11937/bfyy.20241569]
太子参枯萎病对土壤微生物组成和功能的影响
- Title:
- Effects of Fusarium Wilt on Soil Microbial Structure and Function of Pseudostellaria heterophylla
- 文章编号:
- 1001-0009(2024)23-0073-10
- Keywords:
- Pseudostellaria heterophylla; fusarium wilt; community of microbiome; function of microbiome; continuous cropping obstacle
- 分类号:
- R 282.2
- 文献标志码:
- A
- 摘要:
- 以太子参为试材，采用微生物组分析方法，研究了太子参枯萎病对其根际和根区土壤微生物组成和功能的影响，以期明确太子参枯萎病介导其微生物组成变化的微生态机制。结果表明：枯萎病显著改变了太子参根际和根区土壤中细菌群落结构，对土壤中拟杆菌门和髌骨细菌门的细菌具有很强的吸引作用，而对绿弯菌门的细菌具有很强的排除作用；同时枯萎病富集了禾谷镰刀菌、土赤壳菌等病原菌，而排斥了木霉菌等有益真菌。共发生网路结果表明，太子参枯萎病降低了土壤菌群的多样性和稳定性，土壤微生物间向单一化、简单化方向演变，表现为土壤抵御逆境的缓冲能力下降。土壤微生物功能分析结果表明，枯萎病可能通过氨基酸代谢通路的富集增强病原菌的致病力和传播力，枯萎病介导萜类、聚酮类、抗生素类次级代谢物的合成路径功能基因的富集促进对特定有益微生物的排除作用。该研究初步揭示太子参枯萎病介导了连作障碍加重及其原因，为阐释太子参连作障碍的微生态机制奠定基础。
- Abstract:
- Taking Pseudostellaria heterophylla as the test material,the effects of fusarium wilt on the microbial composition and function of P.heterophylla microbiome in the rhizosphere and root zone soil was studied using microbial sequencing,in order to clarify its microecological mechanism that fusarium wilt disease mediated the P.heterophylla microbiome alteration.The results showed that fusarium wilt significantly changed the bacterial community structure in the rhizosphere and root zone soil of P.heterophylla，which had a strong attraction to Bacteroides and Patescibacteria in the soil，while it had a strong elimination effect on Chloroflexi.In addition，Fusarium spp.and Llyonectria spp. were enriched，while beneficial fungus such as Trichoderma sppwere excluded.The cooccurrence network analysis found that fusarium wilt reduced the diversity and stability of soil microbial communities，and soil microorganisms evolved towards simplification，which was manifested by a decrease in the buffering capacity of soil to resist adverse conditions.The analysis of function of microbiome indicate that fusarium wilt may enhance the pathogenicity and transmissibility of pathogen through the enrichment of amino acid metabolic pathways，and mediates the accumulation of terpene，polyketone，and antibiotic secondary metabolites，promoting the elimination of specific beneficial microorganisms.This study preliminarily revealed that the fusarium wilt of P.heterophylla mediated the aggravation of continuous cropping obstacle and its causes，laying a foundation for explaining the microecological mechanism of continuous cropping obstacle of P.heterophylla.
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备注/Memo
第一作者简介：袁青松（1986-），男，博士，副教授，现主要从事中药民族药质量控制与中药材病虫害防治等研究工作。E-mail：yqs198609031006@126.com.责任作者：周涛（1968-），女，博士，教授，现主要从事中药资源学等研究工作。E-mail：taozhou88@163.com.基金项目：国家重点研发计划资助项目（2023YFC3603803）；贵州省科技支撑计划资助项目（黔科合支撑［2022］一般136）；贵州省基础研究计划（自科）资助项目（黔科合基础-ZK［2023］一般415）。收稿日期：2024-04-13