WANG Fanfan,TANG Tao,ZHANG Luna,et al.Effects of Different Factors on Seed Germination and Seedling Growth of Rheum officinale[J].Northern Horticulture,2024,(20):91-99.[doi:10.11937/bfyy.20241227]
不同因素对药用大黄种子萌发和幼苗生长的影响
- Title:
- Effects of Different Factors on Seed Germination and Seedling Growth of Rheum officinale
- 文章编号:
- 1001-0009(2024)20-0091-09
- Keywords:
- Rheum officinale; seeding; soaking time; water content; phytohormone
- 分类号:
- S 35
- 文献标志码:
- A
- 摘要:
- 以三年生药用大黄种子为试材,采用三因素四水平的正交实验设计,研究了浸种时长、基质含水量和激素对药用大黄种子萌发及幼苗特性的影响,以期为药用大黄种苗繁育标准化规模化发展提供参考依据。结果表明:浸种时长为6 h、基质含水量为70%处理的种子出苗率最佳,T16(24 h,70%,赤霉素)的种苗叶面积、株高、地上质量和鲜质量均最佳,T8(12 h,70%,IAA)的种苗茎粗和叶片可溶性蛋白质含量最佳,T7(12 h,60%,水杨酸)的种苗根长和叶片过氧化物酶活性最佳,T10(18 h,50%,水杨酸)的种苗叶片丙二醛(MDA)含量最佳,T5(12 h,40%,6-BA)的种苗叶片超氧化物歧化酶(SOD)活性最佳。相关性分析和极差分析结果表明,药用大黄种苗繁育的理论最佳方案为浸种18 h、基质含水量保持70%和喷施水杨酸。
- Abstract:
- Taking three-year-old seeds of Rheum officinale as the test materials,orthogonal experimental method with three factors and four levels was conducted to study the effects of soaking time,substrate water content and hormones on seed germination and seedling characteristics,in order to provide reference for the standardization and scale development of seedling breeding of R.officinale.The results showed that the seed emergence rate was the best when the soaking time was 6 hours and the substrate water content was 70%.The seedling leaf area,plant height,aboveground weight and fresh weight of T16 (24 hours,70%,gibberellic acid) were the best.T8 (12 hours,70%,IAA) had the best stem diameter and soluble protein content in leaves.The root length and leaf peroxidase activity treated with T7 (12 hours,60%,salicylic acid) were the best.The content of malondialdehyde in leaves treated with T10 (18 hours,50%,salicylic acid) was the best.Superoxide dismutase activity of seedling leaves treated with T5 (12 hours,40%,6-BA) was the best.The results of correlation analysis and range analysis showed that the best theoretical scheme for seedling breeding of R.officinale was soaking seeds for 18 hours,maintaining 70% water content and spraying salicylic acid.
参考文献/References:
[1]国家药典委员会.中华人民共和国药典一部(2020年)[M].北京:中国医药科技出版社,2020.[2]唐涛,王帆帆,郭杰,等.恩施州药用大黄黑粉病鉴定及病区真菌群落结构多样性分析[J].中药材,2021,44(9):2028-2033.[3]冯茜雯,周天华.药用大黄主要病害的识别与防治[J].南方农业,2023,17(22):59-62,103.[4]康胜华,侯璐,朱琳.唐古特大黄根腐病病原菌的鉴定[J].青海农林科技,2023(1):62-66.[5]王瑞霞,闫永刚,王俊,等.不同浸种方式对矮大黄容器育苗出苗率的影响[J].现代农业科技,2021(19):73-74.[6]梁小燕,李依民,高静,等.外源水杨酸对盐胁迫下大黄种子萌发及幼苗生长的影响[J].西北植物学报,2022,42(11):1919-1926.[7]谢丰璞,王楠,高静,等.旱、盐胁迫对不同海拔、生长年限与贮藏条件的药用大黄种子萌发特性的影响[J].生态学杂志,2023,42(1):18-28.[8]韩愈,韦霄,唐辉,等.土壤水分对地枫皮种子萌发与早期幼苗生长的影响[J].种子,2018,37(7):10-15.[9]李军乔,李晨芹,王鑫慈,等.2种外源激素对蕨麻苗期生长的影响[J].种子,2022,41(5):120-127.[10]HE Y,ZHANG M,ZHOU W,et al.Transcriptome analysis reveals novel insights into the continuous cropping induced response in Codonopsis tangshen,a medicinal herb[J].Plant Physiology and Biochemistry,2019,141:279-290.[11]张文龙,罗永慧,严毅,等.贵州特色药材苦参良种繁育体系的建设与思考[J].种子,2018,37(6):76-78.[12]邓丽丽,韦霄,熊忠臣,等.不同处理对赤苍藤种子萌发和幼苗生长的影响[J].广西科学院学报,2023,39(1):78-85.[13]冯聪聪,翟勇,李浩男,等.不同处理对柴胡种子萌发的影响[J].现代农业科技,2020(13):50-51,53.[14]张合瑶,马福仙,李莲芳,等.微波辐射和浸种对华山松种子生活力和发芽的影响[J].西南林业大学学报(自然科学),2023,43(5):23-30.[15]姜雨昕,曾行调,种湘歌,等.不同因素对北苍术种子育苗的影响[J].人参研究,2023,35(2):47-51.[16]李吉涛,陈宗游,唐辉,等.不同环境因子及破壳处理对锥栗种子萌发特性的影响[J].广西植物,2020,40(2):143-150.[17]李廷菊,李灿琳,冉毅,等.不同播期和激素浓度对独一味幼苗生长和药用成分的影响[J].草学,2023(6):49-59.[18]常晖,靳鹏博,杨祎辰,等.不同处理对黄精种子萌发和幼苗生长的影响[J].中国野生植物资源,2023,42(7):32-38.[19]王安虎,杨坪.不同育苗基质及激素处理对华重楼种子出苗的影响研究[J].西昌学院学报(自然科学版),2023,37(1):6-10,35.[20]任鸿濛,李迎超,贾仕军,等.植物生长调节剂浸种对甘草种子萌发的影响[J].北方园艺,2023(17):110-115.[21]周武先,李大荣,龚丝雨,等.外源锌对白术种子萌发及幼苗生长的影响[J].北方园艺,2022(3):98-106.[22]李娟,林海丹,童家赟.何首乌种子萌发条件的优化和育苗条件的筛选[J].北方园艺,2021(23):114-120.
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备注/Memo
第一作者简介:王帆帆(1994-),女,硕士,助理研究员,现主要从事中药材病害防控等研究工作。E-mail:wff164229@163.com.责任作者:游景茂(1983-),男,博士,研究员,现主要从事中药材病虫害防控等研究工作。E-mail:jingmaoyou@126.com.基金项目:恩施州科技计划资助项目(D20220055);国家现代农业产业技术体系建设专项资助项目(CARS-21);湖北省农业科学院青年科技基金资助项目(2023NKYJJ30);湖北省中央引导地方科技发展专项资助项目(2022BGE256);湖北省重点研发资助项目(2022BBA153)。收稿日期:2024-03-25