ZENG Chuntao,ZHANG Shuyue,QIU Xuewen,et al.Effects of Melatonin Soaking on Seed Germination and Physiological Indexes of Cowpea Under Low Temperature Stress[J].Northern Horticulture,2024,(20):8-15.[doi:10.11937/bfyy.20241006]
褪黑素浸种对低温胁迫下豇豆种子萌发和生理指标的影响
- Title:
- Effects of Melatonin Soaking on Seed Germination and Physiological Indexes of Cowpea Under Low Temperature Stress
- 文章编号:
- 1001-0009(2024)20-0008-08
- Keywords:
- cowpea; seed; melatonin; cold stress; physiological characteristics
- 分类号:
- S 643.4
- 文献标志码:
- A
- 摘要:
- 以豇豆品种‘华赣绿秀’的种子为试材，采用浸种的方法，在0、50、100、200 μmol·L-1的褪黑素（MT）浓度条件下，经历了10 ℃的低温环境，研究褪黑素处理对豇豆种子萌芽阶段的发芽率、抗氧化酶活性、超氧阴离子（O2）产生速率、渗透调节物质的含量以及丙二醛（MDA）含量等逆境生理指标的影响，以期为豇豆在设施生产上的应用提供参考依据。结果表明：1）在低温胁迫下，豇豆种子的萌发受到抑制，而施加外源褪黑素可以提高低温胁迫下的种子萌发率，而萌发率仍低于对照。2）豇豆种子中的过氧化物酶（POD）和过氧化氢酶（CAT）活性均在200 μmol·L-1MT处理第4天达到最大值，超氧化物歧化酶（SOD）活性在200 μmol·L-1MT处理第6天达到峰值，而MDA含量在100 μmol·L-1MT处理第6天达到峰值，O2产生速率在50 μmol·L-1MT处理第6天达到最小值。3）游离脯氨酸含量在50 μmol·L-1MT处理第6天达到最大值，可溶性蛋白质含量在100 μmol·L-1MT处理第4天达到最大值，而可溶性糖含量在200 μmol·L-1MT处理第2天达到最大值。研究发现，在低温胁迫条件下施加外源褪黑素可以提高种子的发芽率、增强抗氧化酶活性、减少MDA含量和减缓O2的生成速度，促进渗透调节物质的积累，有效缓解低温胁迫对豇豆种子萌发时的损伤，增强其对低温的耐受性，恢复其正常生长能力，且施加浓度为200 μmol·L-1时效果较好。
- Abstract:
- Taking the seeds of the cowpea variety ‘Huagan Lyuxiu’ as the test materials,under the conditions of 0，50，100 and 200 μmol·L-1 melatonin (MT) concentration,undergoing a low temperature environment of 10 ℃,the germination rate,antioxidant enzyme activity,superoxide anion (O2) production rate,content of osmotic adjustment substances，and MDA content of cowpea seeds were studied under the treatment of melatonin,in order to provide reference for the application of cowpea in facility production.The results showed that,1) germination of cowpea seeds was inhibited under low-temperature stress，whereas the application of exogenous melatonin improved seed germination under low-temperature stress，whereas the germination rate was still lower than that of the control.2) Both peroxidase (POD) and catalase (CAT) activities in cowpea seeds reached a maximum on 4th day of the 200 μmol·L-1MT treatment，superoxide dismutase (SOD) activity peaked on 6th day of the 200 μmol·L-1MT treatment，whereas the MDA content peaked on 6th day of the 100 μmol·L-1MT treatment，and the rate of superoxide anion (O2) production reached a minimum on 6th day of the 50 μmol·L-1 MT treatment.3) The free proline content reached a maximum on 6th day of the 50 μmol·L-1MT treatment，the soluble protein content reached a maximum on 4th day of the 100 μmol·L-1MT treatment，and the soluble sugar content reached a maximum on 2nd day of the 200 μmol·L-1MT treatment.It was found that the application of exogenous melatonin under low-temperature stress conditions could increase the germination rate of seeds，enhance the activity of antioxidant enzymes，reduce the content of MDA，slow down the production rate of superoxide anion (O2)，and promote the accumulation of osmotic-regulating substances，which could effectively alleviate the damage caused by low-temperature stress to cowpea seeds during germination，enhance their tolerance to low temperatures，and restore their normal growth ability.The effect was better when a concentration of 200 μmol·L-1was applied.
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备注/Memo
第一作者简介：曾春涛（2000-），女，硕士研究生，研究方向为蔬菜生理生态与环境调控。E-mail：schizobucia@126.com.责任作者：吴才君（1964-），男，博士，教授，博士生导师，现主要从事蔬菜生理生态与环境调控等研究工作。E-mail：wucj12@126.com.基金项目：江西省农作物良种联合攻关项目专项资金资助项目（赣财农指［2022］7号）。收稿日期：2024-03-12