WANG Xin,JING Ruiyong,XIA Yuqiao,et al.Screening of Culture Substrates and Exploration of Living Condition of Hohenbuehelia serotina[J].Northern Horticulture,2024,(23):105-113.[doi:10.11937/bfyy.20240986]
亚侧耳栽培基质筛选及其生活条件探索
- Title:
- Screening of Culture Substrates and Exploration of Living Condition of Hohenbuehelia serotina
- 文章编号:
- 1001-0009(2024)23-0105-09
- Keywords:
- Hohenbuehelia serotina; straw partial substitution for wood chips; degradation rate of lignocellulose; extracellular enzyme activity; living condition
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以亚侧耳为试材,利用5种作物秸秆替代部分(40%)木屑初步筛选亚侧耳栽培基质,采用单因素试验法,研究了不同培养条件对亚侧耳菌丝生长速率、胞外酶活性、木质素、纤维素、半纤维素降解率及失重率的影响,以期为作物秸秆替代木屑栽培亚侧耳提供参考依据。结果表明:在这5种农作物秸秆中,大豆和玉米秸秆处理的菌丝生长速度与全木屑无差异,综合失重率、纤维素、半纤维素和木质素降解率及5种胞外酶活性等指标,大豆和玉米秸秆可替代部分木屑;单因素试验探索亚侧耳生活条件中,综合所有测定指标分析表明在碳氮比27∶1、pH 7.0、料水比1∶1.8 g·mL-1和22~25 ℃时更有利于亚侧耳菌丝的生长;相关性分析结果表明,菌丝生长速率与胞外酶活性、木质纤维素降解率和失重率之间的偶联性较强。综上所述,亚侧耳栽培部分替代木屑的适宜秸秆为玉米和大豆秸秆,最适生活条件为碳氮比27∶1、pH 7.0、料水比1∶1.8 g·mL-1、温度22~25 ℃。
- Abstract:
- Taking Hohenbuehelia serotina as the test material,culture substrates of Hohenbuehelia serotina were selected by replacing partly (40%) wood chips with 5 kinds of crop straws,the single factor test method was used,effects of different culture conditions on the mycelial growth rate,extracellular enzyme activity,degradation rate of lignin,cellulose and hemicellulose,substrate weight loss rate of Hohenbuehelia serotina were studied,in order to provide reference for cultivating Hohenbuehelia serotina with crop straw partial substitution for wood chips.The results showed that among the five kinds of crop straws,Hohenbuehelia serotina mycelial growth rate were no significant difference between soybean straw,corn straw and all wood chips treatment.Soybean and corn straw can replace part of wood chips according to weight loss rate,degradation rate of cellulose,hemicellulose and lignin,and activity of 5 kinds of extracellular enzymes.In the experiment of exploring the living conditions of Hohenbuehelia serotina with single factor,the ratio of carbon to nitrogen was 27∶1,pH 7.0,the ratio of material to water was 1∶1.8 g·mL-1 and the temperature of 22-25 ℃ by analyzing all indexes measured.Correlation analysis showed that there was a strong coupling between mycelial growth rate and extracellular enzyme activity,lignocellulose degradation rate and weight loss rate.In conclusion,optimal crop straw partial substitution for wood chips were corn and soybean straw,the optimum living conditions of Hohenbuehelia serotina are carbon-nitrogen ratio of 27∶1,pH value of 7.0,material-water ratio of 1∶1.8 g·mL-1 and temperature of 22-25 ℃.
参考文献/References:
[1]辛树权,赵骥民,沈永.不同培养条件对元蘑菌丝生长的影响[J].北方园艺,2013(10):140-142.[2]田风华.中国东北元蘑种质资源评价及其三萜合成途径相关基因研究[D].长春:吉林农业大学,2019.[3]韩金胜,李香雪,蔡晓楼,等.林下元蘑栽培与管理技术[J].中国林副特产,2021(6):37-39,42.[4]LI X,WANG L,WANG Y,et al.Effect of drying method on physicochemical properties and antioxidant activities of Hohenbuehelia serotina polysaccharides[J].Process Biochemistry,2016,51(8):1100-1108.[5]LI X,WANG L,WANG Z.Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina[J].International Journal of Biological Macromolecules,2017,98:59-66.[6]WANG L,LI X,WANG B.The cytotoxicity activity of Hohenbuehelia serotina polyphenols on HeLa cells via induction of cell apoptosis and cell cycle arrest[J].Food and Chemical Toxicology,2019,124:239-248.[7]刘姿彤,钱华,王延锋,等.元蘑富硒栽培试验初报[J].食药用菌,2022,30(6):424-427.[8]SHENG C,WANG Y,PAN C,et al.Evaluation of rice straw,corncob,and soybean straw as substrates for the cultivation of Lepista sordida[J].Life,2024,14(1):101.[9]陈正启,刘林,华蓉,等.我国农作物秸秆在食用菌栽培中的利用现状及应用前景[J].中国食用菌,2023,42(1):1-6.[10]杨慧,邓超,张志昌,等.不同培养料对秋冬季平菇生长的影响[J].南方农业,2021,15(16):40-42.[11]AKTER M,HALAWANI R F,ALOUFI F A,et al.Utilization of agro-industrial wastes for the production of quality oyster mushrooms[J].Sustainability,2022,14(2):994.[12]ZHOU F,HANSEN M,HOBLEY T J,et al.Valorization of green biomass:Alfalfa pulp as a substrate for oyster mushroom cultivation[J].Foods,2022,11(16):2519.[13]孙瑞祥,韩建东,杨树德,等.豆秸与玉米芯复混栽培平菇试验[J].北方园艺,2022(16):128-133.[14]XU S,WANG F,FU Y,et al.Effects of mixed agro-residues (corn crop waste) on lignin-degrading enzyme activities,growth,and quality of Lentinula edodes[J].RSC Advances,2020,10(17):9798-9807.[15]TAGKOULI D,BEKIARIS G,PANTAZI S,et al.Volatile profiling of Pleurotus eryngii and Pleurotus ostreatus mushrooms cultivated on agricultural and agro-industrial by-products[J].Foods,2021,10(6):1287.[16]马银鹏,周舒扬,马庆芳,等.玉米秸秆基质对黑木耳栽培的影响[J].北方园艺,2022(19):122-128.[17]HUANG L,SUN N,BAN L,et al.Ability of different edible fungi to degrade crop straw[J].AMB Express,2019,9(1):4.[18]周宇,刘朋虎,刘波,等.不同栽培配方对茶树菇生长发育及胞外酶活性的研究[J].北方园艺,2023(19):114-121.[19]徐秀红,陈阿敏,裴芸,等.香菇液体和固体菌种在栽培料中胞外酶活性分析[J].分子植物育种,2021,19(20):6933-6941.[20]曹永佳,马鸿飞,崔宝凯,等.不同固体发酵培养基下三种白腐真菌分泌的木质纤维素酶活性[J].菌物学报,2021,40(5):1123-1139.[21]曾春函,王传华.我国主要蜜环菌生物种胞外酶活性对温度的种特异性响应[J].菌物学报,2019,38(2):195-209.[22]胡延如,尼振原,张佳欣,等.培养料不同含氮量和碳氮比对糙皮侧耳生长和营养成分含量的影响[J].食用菌学报,2022,29(1):36-40.[23] 郑林用,刘本洪.食用菌培养料碳氮比(C/N)的计算方法[J].四川农业科技,1997(3):37.[24]王鑫淼,荆瑞勇,吴楠,等.不同基质对桑黄菌丝生长及酶活性的影响[J].北方园艺,2022(9):109-114.[25]宋芳芳,任萍,徐建良,等.水稻秸秆在旱作土壤中的降解过程及降解菌剂施用效果[J].中国土壤与肥料,2015(2):103-110.[26]任鹏飞,刘岩,任海霞,等.秸秆栽培食用菌基质研究进展[J].中国食用菌,2010,29(6):11-14.[27]严晓芳.安吉地区野生秀珍菇的鉴定驯化及其生理学特性研究[D].合肥:安徽农业大学,2023.[28]KACHLISHVILI E,TSIKLAURI N,KHARDZIANI T,et al.Physiological regulation of edible and medicinal higher basidiomycetes lignocellulolytic enzyme activity[J].International Journal of Medicinal Mushrooms,2002,4(2):8.[29]司徒成,余天华,宋宇欣.不同食用菌对猕猴桃枝木质纤维素降解的比较[J].北方园艺,2022(5):111-118.[30]SONG R Q,DENG X.Study on biodegradated ability of thirteen edible fungi to straw[J].Journal of Forestry Research,2004,15(3):223-226.[31]杜静,李坤鹏,钮琰星,等.油菜秸秆替代棉籽壳栽培食用菌的可行性分析[J].食品安全质量检测学报,2015,6(10):4034-4039.[32]CHEN H,LI S,CUI Z,et al.Synergistic degradation of maize straw lignin by manganese peroxidase from Irpex lacteus[J].Applied Biochemistry and Biotechnology,2023,195(6):3855-3871.[33]张秀伟,蔡甫格,吴家丽,等.贵州2株野生侧耳的鉴定、生物学特性及驯化试验[J].中国食用菌,2023,42(4):41-46.[34]简利茹,杜双田,蒋琪,等.基质用量和料水比对蛹虫草子座虫草素产量的影响[J].西北农业学报,2016,25(11):1736-1741.[35]邹莉,王义,王轶,等.亚侧耳菌丝生物学特性研究[J].菌物学报,2008,27(6):915-921.[36]WANG Q,XIAO T,JUAN J,et al.Lignocellulose degradation efficiency of Agaricus bisporus strains grown on wheat straw-based compost[J].Journal of Agricultural and Food Chemistry,2023,71(28):10607-10615.[37]LI G,WANG Y,ZHU P,et al.Functional characterization of laccase isozyme (PoLcc1) from the edible mushroom Pleurotus ostreatus involved in lignin degradation in cotton straw[J].International Journal of Molecular Sciences,2022,23(21):13545.[38]赵翠敏,杜芳,邹亚杰,等.基于不同基质刺芹侧耳菌丝生长与木质素降解酶的相关性研究[J].中国食用菌,2020,39(11):109-114.[39]肇莹,马晓颖,陈珣,等.不同基质栽培蛹虫草营养成分与酶活的相关性分析[J].北方园艺,2021(8):99-105.
备注/Memo
第一作者简介:王鑫(1999-),女,硕士研究生,研究方向为生物与医药。E-mail:2276925632@qq.com.责任作者:王丽艳(1977-),女,博士,副教授,现主要从事食用菌资源开发与利用等研究工作。E-mail:laosan1@126.com.基金项目:黑龙江八一农垦大学研究生创新资助项目(YJSCX2023-Y71);国家自然科学基金资助项目(31870477);黑龙江八一农垦大学三纵三横资助项目(ZRCPY202114)。收稿日期:2024-03-11