JING Zhuoqiong,GUO Zhijie,SUN Qian,et al.Identification of Pathogen Causing Anthracnose of Pepper (Capsicum annuum L.) in Gansu Province[J].Northern Horticulture,2024,(15):1-7.[doi:10.11937/bfyy.20240808]
甘肃省辣椒炭疽病病原菌鉴定
- Title:
- Identification of Pathogen Causing Anthracnose of Pepper (Capsicum annuum L.) in Gansu Province
- 文章编号:
- 1001-0009(2024)15-0001-07
- 关键词:
- 辣椒炭疽病; 分离鉴定; 多基因联合系统发育分析; 松针炭疽菌
- Keywords:
- anthracnose of pepper; isolation and identification; multilocus phylogenetic analysis; Colletotrichum fioriniae
- 分类号:
- S 436.418
- 文献标志码:
- A
- 摘要:
- 以从甘肃省兰州市安宁区采集疑似辣椒炭疽病的发病辣椒果实为试材，采用组织分离法分离病原菌，通过构建多基因联合系统发育树，结合形态学和柯赫氏法则鉴定兰州市辣椒炭疽病病原真菌种类,以期为该病的诊断和防控提供参考依据。结果表明：从具有典型症状的病果上分离得到13株具有相同培养性状的菌株，柯赫氏法则明确代表菌株CA1902为辣椒炭疽病的致病菌；通过ITS、TUB2、ACT、CHS-1、HIS3和GAPDH构建多基因联合系统发育树，该菌株与松针炭疽菌Colletotrichum fioriniae聚在同一分支，且支持率为100%。综合可知，甘肃省兰州市安宁区辣椒炭疽病病原菌种类为松针炭疽菌C.fioriniae，是该种引起甘肃地区辣椒炭疽病的首次报道。考虑到辣椒的经济价值，可依据该鉴定结果制定有效的病害防控措施。
- Abstract:
- Taking diseased chili fruits with typical anthracnose symptoms collected from Anning district，Lanzhou city，Gansu Province as the test materials，taking tissue isolation method to isolate pathogens，a multi gene combined phylogenetic tree，and combining morphology and Koch′s rule were constructed to identify the fungal species causing pepper anthracnose in Lanzhou city,in order to provide reference for the diagnosis and prevention and control of this disease.The results showed that 13 strains with the same culture characteristics were obtained from typical anthracnose pepper fruits.The assay of Koch′s postulates confirmed that CA1902 was the causal agent of pepper anthracnose that were identified as Colletotrichum fioriniae by morphology and multilocus phylogenies from combined datasets of ITS，TUB2,ACT,CHS-1,HIS3 and GAPDH.The isolate CA1902 clustered with C.fioriniae，having 100% bootstrap support.According to the above,the pathogen causing anthracnose of pepper (Capsicum annuum L.) was Colletotrichum fioriniae in Anning district,in Lanzhou,Gansu Province.This study was the first report of C.fioriniae causing anthracnose on pepper in Gansu Province，China.Considering the economic value of pepper，this identification maight aid in the selection of appropriate management tactics for this disease.
参考文献/References:
［1］邹学校,朱凡.辣椒的起源、进化与栽培历史［J］.园艺学报,2022,49(6):1371-1381.［2］王培先,李正南,张磊,等.辣椒种质抗炭疽病水平鉴定及 炭疽病菌药剂敏感性分析［J］.北方园艺,2022(21):1-8.［3］LAKSHMESHA K K,LAKSHMIDEVI N,MALLIKARJUNA S A.Changes in pectinase and cellulase activity of Colletotrichum capsicimutants and their effect on anthracnose disease on capsicum fruit［J］.Archives of Phytopathology and Plant Protection,2005,38(4):267-279.［4］DIAO Y Z,ZHANG C,LIU F,et al.Colletotrichum species causing anthracnose disease of chili in China［J］.Persoonia,2017,38:20-37.［5］XU X,ZHANG Z,WANG Y,et al.First report of Colletotrichum fioriniae causing anthracnose disease of Rhus chinensis in China［J］.Plant Disease,2023,107(12):4034.［6］CHANG J,ZHAI F,ZHANG Y,et al.Identification and characterization of Colletotrichum fioriniae and C.fructicola that cause anthracnose in pecan［J］.Frontiers in Plant Science,2022(13):1043750.［7］HU S,PENG L,DING H,et al.First report of Colletotrichum fioriniae causing anthracnose on Rhododendron delavayi in China［J］.Plant Disease,2022,106(11):2995.［8］XU S J,JING Z Q,GUO Z J,et al.First report of anthracnose disease caused by Colletotrichum fioriniae on barbary wolfberry (Lycium barbarum L.) in Gansu Province,China［J］.Plant Disease,2022,106(9):2523.［9］LAI J L,BAI C H,PAN J M,et al.Colletotrichum horii and Colletotrichum fioriniae causing anthracnose on star anise (Illicium verum) in China［J］.Plant Disease,2023,107(10):3294.［10］WANG Q H,KUANG S Y,ZENG Y C,et al.First report of Colletotrichum fioriniae causing leaf spot on Schima superba in the world［J］.Plant Disease,2022,106(9):2524.［11］LIU H,LI D,ZHANG T,et al.First report of anthracnose on Cinnamomum camphora (camphor tree) caused by Colletotrichum fioriniae and Colletotrichum siamense in China［J］.Plant Disease,2022,106(5):1519.［12］但雨柔,刘铭,崔馨燕,等.多花黄精炭疽病病原菌鉴定［J］.植物保护,2023,49(2):288-293.［13］DAMM U,CANNON P F,WOUDENBERG J H C,et al.The Colletotrichum acutatum species complex［J］.Studies in Mycology,2012,73:37-113.［14］张翠文,魏立娟,杨成德.甘肃省辣椒炭疽病菌的分离鉴定及生物学特性［J］.西北农业学报,2023,32(10):1627-1636.［15］SREENIVASAPRASAD S,TALHINHAS P.Genotypic and phenotypic diversity in Colletotrichum acutatum,a cosmopolitan pathogen causing anthracnose on a wide range of hosts［J］.Molecular Plant Pathology,2005,6(4):361-378.［16］SHIVAS R G,TAN Y P.A taxonomic re-assessment of Colletotrichum acutatum，introducing C.fioriniae comb.et stat.nov.and C.simmondsii sp.nov［J］.Fungal Diversity,2009,39:111-122.［17］MACKENZIE S J,PERES N A,BARQUERO M P,et al.Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida［J］.Phytopathology,2009,99(5):620-631.［18］POLASHOCK J J,OUDEMANS P V,CARUSO F L,et al.Population structure of the North American cranberry fruit rot complex［J］.Plant Pathology,2009,58:1116-1127.［19］IVIC D,VONCINA D,SEVER Z,et al.Identification of Colletotrichum species causing bitter rot of apple and pear in Croatia［J］.Journal of Phytopathology,2013,161(4):284-286.［20］MUNDA A.First report of Colletotrichum fioriniae and C.godetiae causing apple bitter rot in Slovenia［J］.Plant Disease,2014,98(9):1282.［21］NODET P,BARONCELLI R,FAUGRE D,et al.First report of apple bitter rot caused by Colletotrichum fioriniae in Brittany,France［J］.Plant Disease,2016,100(7):1497.［22］TASHIRO N,URAKAWA A,SHOJI K,et al.Postharvest anthracnose on Satsuma mandarin orange caused by Colletotrichum fioriniae［J］.Journal of General Plant Pathology,2018,84(5):381-385.［23］LING J F,PENG A T,JIANG Z D,et al.First report of anthracnose fruit rot caused by Colletotrichum fioriniae on litchi in China［J］.Plant Disease,2021,105(4):1225.［24］NIGAR Q,CADLE-DAVIDSON L,GADOURY D M,et al.First report of Colletotrichum fioriniae causing grapevine anthracnose in New York［J］.Plant Disease,2022,1077(1):223.［25］崔治家,邵晶,马毅,等.甘肃省道地药材枸杞子资源现状及产业发展对策研究［J］.中国现代中药,2023,9(1):15-21.［26］马琪,祁伟伟,杨盈,等.天水市苹果产业发展现状及对策［J］.北方果树,2023(3):50-51,59.
相似文献/References:
[1]秦刚,吴庆丽.辣椒炭疽病生防菌筛选及抗菌活性初步研究[J].北方园艺,2012,36(06):124.
QIN Gang,WU Qing-li.The Intial Screen of Biological Control and Study of Antimicrobial Activity of Capsicum Anthracnose[J].Northern Horticulture,2012,36(15):124.
[2]马 荣 群,黄 粤,宋 正 旭,等.辣 椒 炭 疽 病 抗 性 资 源 筛 选[J].北方园艺,2008,32(09):0.[doi:10.11937/bfyy.200809077]
[J].Northern Horticulture,2008,32(15):0.[doi:10.11937/bfyy.200809077]
[3]李艳青,赵玉翠,潘好芹.山东寿光茄子褐纹病病原鉴定及药效试验[J].北方园艺,2017,41(09):106.[doi:10.11937/bfyy.201709023]
LI Yanqing,ZHAO Yucui,PAN Haoqin.Pesticide Effect Experiment and Identification of Phomopsis vexans From Eggplant in Shouguang of Shandong Province[J].Northern Horticulture,2017,41(15):106.[doi:10.11937/bfyy.201709023]
[4]赵欢.药用植物内生真菌的研究进展[J].北方园艺,2017,41(13):170.[doi:10.11937/bfyy.20163646]
ZHAO Huan.Advances in Endophytic Fungi From Medicinal Plant[J].Northern Horticulture,2017,41(15):170.[doi:10.11937/bfyy.20163646]
[5]肖敏,曾向萍,严婉荣,等.八种药剂对节瓜炭疽病菌的毒力测定与田间防效[J].北方园艺,2017,41(23):16.[doi:10.11937/bfyy.20172394]
XIAO Min,ZENG Xiangping,YAN Wanrong,et al.Toxicity Test of Eight Fungicides to Chiehqua Colletotrichum and Control Effect in Fields[J].Northern Horticulture,2017,41(15):16.[doi:10.11937/bfyy.20172394]
[6]金忠民,赵婧佟,温昱晨,等.耐铅镉菌株的筛选及其生长条件的优化[J].北方园艺,2017,41(24):65.[doi:10.11937/bfyy.20171122]
JIN Zhongmin,ZHAO Jingtong,WEN Yuchen,et al.Screening Strains Resistant of Lead and Cadmium and Growth Conditions Optimization[J].Northern Horticulture,2017,41(15):65.[doi:10.11937/bfyy.20171122]
[7]李小六,范永山,宋彦超,等.大花蕙兰菌根显微结构及内生真菌的分离与鉴定[J].北方园艺,2018,42(08):92.[doi:10.11937/bfyy.20173259]
LI Xiaoliu,FAN Yongshan,SONG Yanchao,et al.Mycorrhizal Microstructure of Cymbidium hybridum and Isolation and Identification of Its Mycorrhizal Fungi[J].Northern Horticulture,2018,42(15):92.[doi:10.11937/bfyy.20173259]
[8]程欢欢,姚伟伟,彭丽娟,等.一株辣椒炭疽病生防芽孢杆菌的筛选[J].北方园艺,2019,43(23):6.[doi:10.11937/bfyy.20191145]
CHENG Huanhuan,YAO Weiwei,PENG Lijuan,et al.Screening of an Antagonistic Bacillus Strain Against Pepper Anthracnose Disease[J].Northern Horticulture,2019,43(15):6.[doi:10.11937/bfyy.20191145]
[9]刘国丽,李红,张敏.人工栽培蛹虫草真菌病害的分离与鉴定[J].北方园艺,2020,44(04):129.[doi:10.11937/bfyy.20192894]
LIU Guoli,LI Hong,ZHANG Min.Isolation and Identification of Fungal Disease in Cordyceps militaris Cultivation[J].Northern Horticulture,2020,44(15):129.[doi:10.11937/bfyy.20192894]
[10]陈少先,刘悦,曾瑶英,等.鹰嘴桃采后病原菌的分离与鉴定[J].北方园艺,2021,(17):108.[doi:10.11937/bfyy.20210293]
CHEN Shaoxian,LIU Yue,ZENG Yaoying,et al.Isolation and Identification of Pathogenic Bacteria of Olecranon Peach After Harvest[J].Northern Horticulture,2021,(15):108.[doi:10.11937/bfyy.20210293]
备注/Memo
第一作者简介：荆卓琼（1984-），女，硕士，助理研究员，现主要从事植物病害与生物防控等研究工作。E-mail：372367645@qq.com.责任作者：徐生军（1984-），男，博士，副研究员，现主要从事植物病害与生物防控等研究工作。E-mail：xusj1001@aliyun.com.基金项目：国家重点研发计划资助项目（2021YFD1400202）；兰州市科技计划资助项目（2021-1-174）；国家自然科学基金资助项目（32060634）；甘肃省农业科学院农业科技创新专项资助项目（2021GAAS54）；甘肃省农业科学院科技成果转化资助项目（2021GAAS-CGZH01）。收稿日期：2024-02-27