HOU Guowen,HAN Guojun,ZANG Longfei,et al.Effects of Amino Acid Fertilizer Combined With Urea on Nitrogen Components and Enzyme Activities of Pepper Soil[J].Northern Horticulture,2024,(19):73-80.[doi:10.11937/bfyy.20240774]
氨基酸肥与尿素配施对辣椒土壤有机氮组分与酶活性的影响
- Title:
- Effects of Amino Acid Fertilizer Combined With Urea on Nitrogen Components and Enzyme Activities of Pepper Soil
- 文章编号:
- 1001-0009(2024)19-0073-08
- Keywords:
- amino acid fertilizer; water-soluble fertilizer; organic nitrogen component; enzyme activity
- 分类号:
- S 614.3
- 文献标志码:
- A
- 摘要:
- 以制干辣椒为试材，采用田间氨基酸肥与尿素配施试验，在相同施氮量下设置7个施肥处理，分别为不施肥（CK）、单施尿素（M0）、20%氨基酸肥+80%尿素（M20）、40%氨基酸肥+60%尿素（M40）、60%氨基酸肥+40%尿素（M60）、80%氨基酸肥+20%尿素（M80）、100%氨基酸肥（M100），测定辣椒收获期0~20、20~40 cm土层中有机氮组分的含量，以及土壤蛋白酶、脲酶、硝酸还原酶和亚硝酸还原酶活性，研究氨基酸肥与尿素配施对土壤有机氮组分及土壤氮素相关酶活性的影响，并分析有机氮组分与土壤酶活性的相关性，以期为辣椒生产中合理施用氨基酸肥提供参考依据。结果表明：配施氨基酸肥能显著提高土壤有机氮组分含量，其中酸解铵态氮>氨基酸态氮>酸解未知态氮>未酸解氮>氨基糖态氮。当施用氨基酸肥比例增加时，同一土层的总氮、氨基酸态氮、铵态氮、未知态氮含量以及脲酶、蛋白酶和硝酸还原酶的活性均为先上升后下降。在有机氮组分中，除氨基糖态氮和未酸解氮外，其他处理在M20时达最大；土壤酶活性中，除蛋白酶M20处理最大外，其他处理在M60达最大。相关性分析表眀，酸解总氮与全氮含量呈显著正相关，而酸解总氮与未酸解氮含量呈显著负相关，氨基糖态氮、酸解未知态氮和全氮含量与4种酶活性均呈显著正相关。综上所述，M20处理土壤供氮潜力最高，能够有效提高辣椒氮肥利用效率。
- Abstract:
- Taking dried pepper as the test material，the combined application of amino acid fertilizer and urea in the field was used.Under the same nitrogen application，seven fertilization treatments were set,no fertilizer (CK)，single urea (M0)，20% amino acid fertilizer+80% urea (M20)，40% amino acid fertilizer+60% urea (M40)，60% amino acid fertilizer+40% urea (M60)，80% amino acid fertilizer+20% urea (M80)，100% amino acid fertilizer (M100).The contents of organic nitrogen components in 0-20 cm and 20-40 cm soil layers at the harvest stage of pepper and soil protease，urease，nitrate reductase and nitrite reductase activity were determined.The effects of combined application of amino acid fertilizer and urea on soil organic nitrogen components and soil nitrogen-related enzyme activities were studied，and the correlation between organic nitrogen components and soil enzyme activities was analyzed，in order to provide reference for the application of amino acid fertilizer in pepper production.The results showed that combined application of amino acid fertilizer significantly increased the content of soil organic nitrogen components，with the order of ammonium nitrogen>amino acid nitrogen>hydrolysable unknown nitrogen>unhydrolyzable nitrogen>amino sugar nitrogen.When the amount of amino acid fertilizer application increased，the total nitrogen，amino acid nitrogen，ammonium nitrogen，unknown nitrogen，urease，protease，and nitrate reductase activities in the same soil layer would first increase and then decrease.Among the organic nitrogen components，except for amino sugar nitrogen and unhydrolyzable nitrogen，the highest values were observed in treatment M20；among the soil enzyme activities，except for the maximum protease activity in treatment M20，the other treatments reached maximum values at M60.The correlation analysis showed that the content hydrolyzed total nitrogen was significantly positively correlated with total nitrogen，and significantly negatively correlated with unhydrolyzable nitrogen.Amino sugar nitrogen，hydrolyzed unknown nitrogen，and total nitrogen were significantly positively correlated with the four enzyme activities.In conclusion，treatment M20 had the highest soil nitrogen supply potential and could effectively improve the nitrogen use efficiency of pepper plants.
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备注/Memo
第一作者简介：候国文(1998-)，男，硕士研究生，研究方向为农业资源高效利用。E-mail:1849196867@qq.com.责任作者：韩国君(1977-)，男，博士，副教授，硕士生导师，现主要从事农业资源利用等研究工作。E-mail:hangj@gsau.edu.cn.基金项目：国家自然科学基金资助项目(31960631)。收稿日期：2024-02-25