ZHOU Lanlan,HU Shuangming,SHANG Xiaohua,et al.Experimental on the Isolation of Wild Morchella Fungi in Gannan Prefecture and the Screening of Mother Culture Medium[J].Northern Horticulture,2024,(17):118-124.[doi:10.11937/bfyy.20240746]
甘南州野生羊肚菌菌种分离及母种培养基筛选试验
- Title:
- Experimental on the Isolation of Wild Morchella Fungi in Gannan Prefecture and the Screening of Mother Culture Medium
- 文章编号:
- 1001-0009(2024)17-0118-07
- Keywords:
- Gannan prefecture; wild morel mushroom; strain isolation; screening of mother culture medium
- 分类号:
- S 646;S 544
- 文献标志码:
- A
- 摘要:
- 以采自甘南州境内（舟曲县、临潭县、迭部县）的野生羊肚菌子实体为试材，采用组织分离方法，研究了5种培养基配方对羊肚菌菌丝生长情况（萌发时间、生长速度、菌丝长势）的影响，并结合不同培养基菌丝在显微镜下的表现，筛选出适宜野生羊肚菌生长的最佳培养基，同时通过出菇试验，初步筛选出优良菌株，以期为甘南州食用菌种业自主创新提供坚实的种质基础和种源支撑。结果表明：PDA培养基为羊肚菌菌丝生长的适宜培养基，菌丝生长速度快，菌丝浓密，菌核形成早，在显微镜下菌丝呈树状，有分支。出菇试验结果显示，ZD和LB为优良菌株，可以进一步进行试验。
- Abstract:
- Taking wild Morchella fruiting bodies collected from within Gannan prefecture (Zhouqu county，Lintan county，Diebu county) as the experimental materials,tissue separation methods were used，the effects of five culture medium formulations on the mycelial growth of Morchella (germination time，growth rate，mycelial growth momentum)，and combined the performance of different culture medium mycelium under the microscope were investigated，the best culture medium suitable for the growth of wild Morchella were screenech.At the same time，through mushroom emergence experiments，excellent strains were preliminarily screened，in order to provide a solid germplasm foundation and source support for independent innovation in the edible mushroom industry in Gannan prefecture.The results showed that PDA medium was a suitable medium for the growth of Morchella mycelium，with fast growth rate，dense mycelium，early formation of fungal nuclei，and branching mycelium under a microscope.The results of the mushroom emergence test showed that ZD and LB were excellent strains and could be further tested.
参考文献/References:
［1］肉斯塔木·艾买提,李敏奇,闫兴富,等.不同外源营养袋对羊肚菌产量的影响［J］.北方园艺,2022(15):126-131.［2］李翔,邓杰,徐宏,等.响应面法优化羊肚菌红枣复合营养粉的加工工艺［J］.食品研究与开发,2019,49(17):58-63.［3］李卫东.羊肚菌生物活性成分及药理作用的研究进展综述［J］.食药用菌,2018,26(3):157-160.［4］李小林,陈诚,黄羽佳,等.顶空固相微萃取-气质联用分析4种野生食用菌干品的挥发性香气成分［J］.食品与发酵工业,2015,41(9):174-180.［5］张广伦,肖正春.新疆野生粗炳羊肚菌的化学成分分析［J］.江苏食用菌,1992(5):21-22.［6］李烨,温鲁.羊肚菌的研究与开发［J］.中国食用菌,2004,23(1):6-7,10.［7］林彬,刘凤梅,刘宜敏.粗柄羊肚菌蛋白质及氨基酸分析［J］.食用菌学报,1996,3(4):21-24.［8］孙晓明,张卫明,吴素玲,等.羊肚菌抗疲劳作用研究［J］.中国野生植物资源,2001,20(1):17-18,32.［9］朱林,程显好,田吉腾.羊肚菌的研究进展［J］.安徽农业科学,2008,36(10):4054-4057.［10］谢占玲,谢占青.羊肚菌研究综述［J］.青海大学学报(自然科学版),2007,25(2):36-40.［11］周兰兰,王海峰,尚晓花,等.甘肃省甘南州野生羊肚菌研究现状及展望［J］.北方园艺,2023(21):138-143.［12］肖江勇,何秀霞,王丹,等.ELISA鉴别条件下的野生羊肚菌菌种分离方法［J］.中国食用菌,2015,34(3):11-14.［13］李玉.野生食用菌菌种分离与鉴定［D］.福州:福建农林大学,2008.［14］许峰,刘宇,王守现,等.一株野生大型真菌的分离与鉴定［J］.中国农学通报,2012,28(13):176-179.［15］段福文,闫尔葵.楚雄地区4种野生菌子实体生长过程调查报告［J］.中国食用菌,2015,34(1):34-36.［16］王建瑞,刘宇,图力古尔.山东省大型真菌物种濒危程度与优先保育评价［J］.生态学报,2015,35(3):837-848.［17］张压宝.马龙县食用菌产业科技创新的现状及对策研究［J］.中国食用菌,2015,34(3):78-81.［18］刘伟,冯丽,张亚平.新疆羊肚菌rDNA的ITS序列分析［J］.石河子大学学报(自然科学版),2009,27(5):584-587.［19］李梦桐,王楠,玛依拉·吐尔地别克,等.羊肚菌栽培及富硒控制技术研究进展［J］.江苏农业科学,2021,49(6):20-27.［20］胡志强,王鑫,王旭,等.松杉灵芝母种培养基筛选及菌株对比试验［J］.中国林副特产,2019(6):36-38.［21］杜忠伟,胡志强,亢学平,等.一种羊肚菌的菌种分离和母种培养基筛选［J］.中国林副特产,2017(6):25-27.［22］马庆芳,张丕奇,马银鹏,等.寒地越冬模式下的羊肚菌生产菌株筛选［J］.北方园艺,2023(8):115-120.［23］冯连荣,张妍,赵鑫闻,等.一株野生黄伞的菌种分离、鉴定及生物学特性［J］.北方园艺,2022(21):115-122.［24］宋立志,冯连荣,梁德军,等.一株野生大型真菌的菌种分离及ITS序列鉴定［J］.安徽农业科学,2018,46(14):113-115.［25］郑旋,康超,杨玲,等.不同培养条件对六妹羊肚菌菌丝形态的影响［J］.中国食用菌,2020,39(12):53-62.［26］邱奉同,王轶.白灵菇母种培养基筛选研究［J］.北方园艺,2008(3):231-232.［27］果禹鑫,尹玉婷,纪鑫梦,等.北京地区野生羊肚菌分离与鉴定［J］.北方园艺,2018(13):145-152.［28］谢放,夏樱霞,苏强军,等.中国被毛孢三种菌丝形态的超显微特征观察［J］.浙江农业学报,2021,33(5):855-860.［29］罗友梅,陈瑞雅,李小勇,等.智能手机+显微镜原位观察食用菌菌丝的操作方法［J］.食药用菌,2021,29(6):539-542.［30］赵航轲,彭浩,卢雨欣.汉中地区羊肚菌人工栽培菌种初步选育研究［J］.中国食用菌,2019,38(8):17-20,27.［31］金琳山,陈波,王晓敏,等.羊肚菌新品种‘黔羊肚菌1号’［J］.园艺学报,2023,50(S1):91-92.
相似文献/References:
[1]果禹鑫,尹玉婷,纪鑫梦,等.北京地区野生羊肚菌分离与鉴定[J].北方园艺,2018,42(13):145.[doi:10.11937/bfyy.20174507]
GUO Yuxin,YIN Yuting,JI Xinmeng,et al.Isolation and Identification of Wild Morels in Beijing[J].Northern Horticulture,2018,42(17):145.[doi:10.11937/bfyy.20174507]
[2]周兰兰,王海峰,尚晓花,等.甘肃省甘南州野生羊肚菌研究现状及展望[J].北方园艺,2023,(21):138.[doi:10.11937/bfyy.20231725]
ZHOU Lanlan,WANG Haifeng,SHANG Xiaohua,et al.Research Status and Prospects of Wild Morchella in Gannan Prefecture，Gansu Province[J].Northern Horticulture,2023,(17):138.[doi:10.11937/bfyy.20231725]
[3]周兰兰,尚晓花,尚红梅,等.乡村振兴背景下甘肃省甘南州食用菌产业转型升级对策研究[J].北方园艺,2024,(12):134.[doi:10.11937/bfyy.20234169]
ZHOU Lanlan,SHANG Xiaohua,SHANG Hongmei,et al.Research on the Transformation and Upgrading Strategies of the Edible Mushroom Industry in Gannan Prefecture，Gansu Province Under the Background of Rural Revitalization[J].Northern Horticulture,2024,(17):134.[doi:10.11937/bfyy.20234169]
备注/Memo
第一作者简介：周兰兰（1987-），女，硕士，高级农艺师，现主要从事食用菌栽培技术等研究工作。E-mail:3403245047@qq.com.基金项目：甘南州2022年度重点产业领域科技计划资助项目(2022JY2NC010)。收稿日期：2024-02-22