GENG Yiran,SUN Jixia,ZHANG Yingjie,et al.Effect of GA3 and 6-BA on Breaking Dormancy and Promoting Germination of Rose Seeds[J].Northern Horticulture,2024,(20):46-54.[doi:10.11937/bfyy.20240654]
GA3及6-BA浸种对月季种子破眠促萌效果研究
- Title:
- Effect of GA3 and 6-BA on Breaking Dormancy and Promoting Germination of Rose Seeds
- 文章编号:
- 1001-0009(2024)20-0046-09
- Keywords:
- Chinese rose; seed germination; hormone treatment; endogenous hormone; key gene expression
- 分类号:
- S 685.12
- 文献标志码:
- A
- 摘要:
- 以不同浓度的赤霉素（GA3）及细胞分裂素（6-BA）浸种处理后的低维护月季品种‘荣光’的种子为试材，采用酶联免疫吸附法测定低温层积（冰箱4 ℃）90 d后0、50、100、150、200 mg·L-1的GA3及6-BA处理下月季种子内源激素GA、脱落酸（ABA）、生长素（IAA）、细胞分裂素（CTK）含量以及激素相关关键目的基因的相对表达量，研究不同浓度的GA3及6-BA处理对‘荣光’种子破眠促萌的影响，以期为筛选出打破月季种子休眠的最适激素浓度提供参考依据。结果表明：不同浓度GA3及6-BA处理的月季种子发芽势及发芽率存在显著差异，150 mg·L-1GA3和50 mg·L-16-BA浸种的发芽率（55.56%、46.67%）最高。与对照相比，GA3及6-BA处理后的月季种子发芽过程中的ABA含量显著降低，GA3、IAA、CTK 含量均显著增加。不同浓度GA3及6-BA处理月季种子发芽过程中的GA3+IAA+CTK/ABA比值均显著高于对照，150 mg·L-1GA3和100 mg·L-16-BA处理下比值最高，说明GA3或6-BA浸种可通过上调GA+IAA+CTK/ABA比值，进而促进月季种子发芽，最适浓度为150 mg·L-1GA3或50~100 mg·L-16-BA处理；激素处理下月季种子激素合成关键基因呈现不同变化，可能通过下调GA2ox、ABI5转录，上调ABI4、WRKY13转录，实现拮抗ABA的作用，促进种子萌发。
- Abstract:
- The seeds of low-maintenance rose variety ‘Glory’ after soaking with different concentrations of GA3 and 6-BA were used as experimental materials.The contents of endogenous hormones GA，ABA，IAA and CTK in rose seeds treated with 0，50，100，150 mg·L-1 and 200 mg·L-1 GA3 and 6-BA and the relative expression levels of hormone-related key genes were determined by enzyme-linked immunosorbent assay (ELISA) after 90 days of low temperature stratification (refrigerator 4 ℃).The effects of different concentrations of GA3 and 6-BA on breaking dormancy and promoting germination of ‘Glory’ seeds were studied，in order to provide reference for screening the optimal hormone concentration to break the dormancy of rose seeds.The results showed that there were significant differences in germination potential and germination rate of rose seeds treated with different concentrations of GA3 and 6-BA，and the germination rates of seeds soaked with 150 mg·L-1GA3 and 50 mg·L-16-BA were the highest (55.56% and 46.67%).Compared with the control，the content of ABA in rose seeds after GA3 and 6-BA treatment was decreased，while the contents of GA，IAA and CTK were significantly increased.The ratio of GA+IAA+CTK/ABA in different concentrations of GA3 and 6-BA treatment was significantly higher than that in the control，and the ratio was the highest under 150 mg·L-1GA3 and 100 mg·L-16-BA treatment，indicated that the ratio of GA+IAA+CTK/ABA could be increased by adjusting GA3 or 6-BA soaking.The optimal concentration was 150 mg·L-1GA3 or 50-100 mg·L-16-BA.The key genes of hormone synthesis in rose seeds showed different changes under hormone treatment，which may achieve the effect of antagonizing ABA and promoting seed germination by down-regulating the transcription of GA2ox and ABI5 and up-regulating the transcription of ABI4 and WRKY13.
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备注/Memo
第一作者简介：耿一然（1999-），女，硕士研究生，研究方向为月季栽培管理与生理生化。E-mail：712715793@qq.com.责任作者：郭文姣（1982-），女，本科，高级农艺师，现主要从事花卉遗传育种与栽培等研究工作。E-mail：467623413@qq.com.基金项目：山东省重点研发计划资助项目（2022LZGCQY009）；烟台市科技发展计划资助项目（2021NYNC014）。收稿日期：2024-02-15