ZHANG Tiantian,LI Yanling,HAO Ruijie.Alleviative Effect of Exogenous SA and PGPB on the Growth Physiology of Antirrhinum majus Under Low Temperature and Weak Light Stress[J].Northern Horticulture,2024,(17):51-60.[doi:10.11937/bfyy.20240548]
外源水杨酸和植物促生菌对低温弱光胁迫下金鱼草生长及生理的缓解效应
- Title:
- Alleviative Effect of Exogenous SA and PGPB on the Growth Physiology of Antirrhinum majus Under Low Temperature and Weak Light Stress
- 文章编号:
- 1001-0009(2024)17-0051-10
- Keywords:
- plant growth promoting bacteria; exogenous salicylic acid; Antirrhinum majus; photosynthesis; ASA-GSH
- 分类号:
- S 682.19
- 文献标志码:
- A
- 摘要:
- 以金鱼草为试材，在低温弱光（5 ℃，光照强度100 μmol·m-2·s-1）条件下处理金鱼草，研究不同浓度水杨酸（SA）和植物促生菌（PGPB）配施对金鱼草幼苗生长及生理指标的影响，以期为增强金鱼草抗低温弱光环境的特性提供参考依据。结果表明：低温弱光胁迫下金鱼草生长受抑制，光合作用下降，ASA-GSH循环受阻，而低温弱光胁迫下添加各浓度SA以及SA+PGPB处理对金鱼草生长均具有显著的促生效应，明显提高金鱼草的株高、茎粗、叶面积、总生物量和根系活力。低温弱光胁迫下，2 mmol·L-1 SA+PGPB处理的金鱼草叶绿素a、叶绿素b、总叶绿素（a+b）、类胡萝卜素含量分别比对照处理增加了162.1%、128.6%、149.0%和102.3%，金鱼草Fv/Fm、Fv/Fo和ΦPSⅡ分别比对照增加136.4%、57.4%和200.0%，NPQ则下降47.6%；金鱼草气体交换参数Pn、Tr和Gs分别增加107.7%、237.5%和91.8%，Ci则下降30.4%；低温弱光胁迫下2 mmol·L-1 SA+PGPB对金鱼草提高ASA、GSH、ASA/DHA以及GSH/GSSG的效果最好，而抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、单脱氢抗坏血酸还原酶（MDHAR）以及谷胱甘肽还原酶（GR）活性也达到常温常光处理下的水平。结果可知，低温弱光胁迫下各浓度SA或SA+PGPB处理能够通过增加叶绿素含量，提高根系活力，改善植物光合特性以及叶绿素荧光参数，提高ASA-GSH相关抗氧化物质以及酶活性来促进植物生长，其中以2 mmol·L-1 SA+PGPB缓解金鱼草受低温弱光胁迫的效果最好。
- Abstract:
- Antirrhinum majus was used as the test material,normal temperature and light (25 ℃,light intensity 600 μmol·m-2·s-1) as the control,A.majus was treated under low temperature and weak light conditions (5 ℃,light intensity 100 μmol·m-2·s-1).The effects of different concentrations of salicylic acid (SA) and plant growth promoting bacteria (PGPB) on the growth physiology of A.majus seedlings were studied,in order to provide reference for the cultivation of A.majus low temperature and weak light stress.The results showed that under low temperature and weak light stress,the growth of A.majus was inhibited,photosynthesis decreased,and the ASA-GSH cycle was hindered.However,under low temperature and weak light stress,the addition of various concentrations of SA and SA+PGPB had a significant promoting effect on the growth of A.majus,significantly improving its plant height,stem diameter,leaf area,total biomass,and root vitality.Under low temperature and weak light stress,the content of chlorophyll a,chlorophyll b,total chlorophyll (a+b),and carotenoids in A.majus treated with 2 mmol·L-1SA+PGPB increased by 162.1%,128.6%,149.0%,and 102.3%,respectively,compared to the control treatment.The Fv/Fm,Fv/Fo,and Φ PS Ⅱ increased by 136.4%,57.4%,and 200.0% respectively compared to the control,while NPQ decreased by 47.6%.The gas exchange parameters Pn,Tr,and Gs of A.majus increased by 107.7%,237.5%,and 91.8%,respectively,while Ci decreased by 30.4%.Under low temperature and weak light stress,2 mmol·L-1SA+PGPB showed the best effect on the improvement of ASA,GSH,ASA/DHA,and GSH/GSSG in A.majus,while the activities of ascorbate peroxidase (APX),dehydroascorbate reductase (DHAR),monodehydroascorbate reductase (MDHAR),and glutathione reductase (GR) also reached the level under normal temperature and light treatment.The results showed that under low temperature and weak light stress,treatments with SA or SA+PGPB at various concentrations could promote plant growth by increasing chlorophyll content,improving root vitality,improving plant photosynthetic characteristics and chlorophyll fluorescence parameters,enhancing ASA-GSH related antioxidant substances and enzyme activity.Among them,2 mmol·L-1 SA+PGPB had the best effect in alleviating low temperature and weak light stress on A.majus.
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备注/Memo
第一作者简介：张甜甜（1986-），女，硕士，讲师，现主要从事园林植物应用与生理等研究工作。E-mail：Zhsfzs0124@163.com.基金项目：2021年山西省高等学校教学改革创新资助项目（Z2021059）；2023年山西省高等学校一般性教学改革创新立项资助项目(J20231736)；山西农业大学2023年度“特”“优”农业高质量发展科技支撑工程资助项目（TYGC23-63）；国家自然科学基金资助项目（41907012）。收稿日期：2024-02-01