XI Jinshan,BAO Xingcheng,YAO Dongdong,et al.Effects of Multi-walled Carbon Nanotubes on the Quality and Coloration of ‘Summer Black’ Grape Berries[J].Northern Horticulture,2024,(13):26-34.[doi:10.11937/bfyy.20240509]
多壁碳纳米管对‘夏黑’葡萄果实品质及着色的影响
- Title:
- Effects of Multi-walled Carbon Nanotubes on the Quality and Coloration of ‘Summer Black’ Grape Berries
- 文章编号:
- 1001-0009(2024)13-0026-09
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 以九年生‘夏黑’葡萄为试材,采用多壁碳纳米管(MWCNTs)对‘夏黑’葡萄叶面以及果实表面进行喷施的方法,分别设置0 mg·L-1(CK)、4 mg·L-1(M1)、8 mg·L-1(M2)、12 mg·L-1(M3)MWCNTs 4个处理,研究了MWCNTs对‘夏黑’葡萄果实品质的影响,筛选出提高‘夏黑’葡萄果实品质的最佳MWCNTs浓度,以期为纳米材料在葡萄生产中的应用提供参考依据。结果表明:MWCNTs显著提高了葡萄果实质量、纵径。处理后第35天,M1、M2、M3处理的果实色泽指数(CIRG)、固酸比、花色苷含量、苯丙氨酸裂解酶(PAL)及查尔酮异构酶(CHI)活性均高于CK,果皮叶绿素含量及可滴定酸含量低于CK。处理后第35天,M2处理的果实色泽指数、可溶性固形物含量、pH、固酸比、花色苷含量、PAL活性、CHI活性均显著高于CK 7.7%、5.9%、3.8%、26.6%、19.7%、25.22%、16.55%;可滴定酸含量、叶绿素含量分别比CK低16%、16%。综合得分表明M2>M1>M3>CK。对‘夏黑’葡萄喷施8 mg·L-1MWCNTs(M2)对其综合品质提升效果最好。
- Abstract:
- Nine-year-old ‘Summer Black’ grapes were used as the test materials,using the method of spraying the foliage as well as the fruit surface of ‘Summer Black’ grapes using multi-walled carbon nanotubes (MWCNTs),with four treatments of 0 mg·L-1 (CK),4 mg·L-1 (M1),8 mg·L-1 (M2),and 12 mg·L-1 (M3) MWCNTs in four treatments.The effects of MWCNTs on the fruit quality of ‘Summer Black’ grapes were investigated,and the optimal concentration of MWCNTs to improve the fruit quality of ‘Summer Black’ grapes was screened,in order to provide reference for the application of nanomaterials in grape production.The results showed that MWCNTs significantly increased grapevine fruit quality and longitudinal diameter.At the 35th day after treatment,fruit color index (CIRG),solid-acid ratio,anthocyanin content,PAL and CHI activity were higher in M1,M2 and M3 treatments than in CK,and pericarp chlorophyll content and titratable acid content were lower than in CK.At the 35th day after treatment,fruit color index,soluble solids content,pH,solid-acid ratio,anthocyanin content,PAL enzyme activity,and CHI enzyme activity were all significantly higher in the M2 treatment than in CK,being 7.7%,5.9%,3.8%,26.6%,19.7%,25.22%,and 16.55% higher,respectively,than in CK.Also titratable acid content and chlorophyll content were lower than CK,16% and 16% lower than CK,respectively.The composite score indicated that M2>M1>M3>CK.Spraying ‘Summer Black’ grapes with 8 mg·L-1 MWCNTs (M2) had the best effect on their overall quality improvement.
参考文献/References:
[1]郑秋玲,刘珅坤,崔万锁,等.不同树形及花穗整形长度对夏黑葡萄果实品质的影响[J].中国农学通报,2019,35(2):53-56.[2]胡容平,范中菡,董义霞,等.褪黑素对葡萄果实品质的影响[J].北方园艺,2022(4):39-44.[3]何堂熹.广西百色地区芒果低产园改造主要栽培技术研究[D].南宁:广西大学,2020.[4]张颖.四个桃品种生物学特性及果实生长发育规律研究[D].长沙:中南林业科技大学,2021.[6]de MARCHI L,NETO V,PRETTI C,et al.Effects of multi-walled carbon nanotube materials on Ruditapes philippinarum under climate change:The case of salinity shifts[J].Aquatic Toxicology,2018,199:199-211.[7]TIAN F,CHEN W,WU C E,et al.Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films[J].International Journal of Biological Macromolecules,2019,126:917-925.[8]VERMA S K,DAS A K,GANTAIT S,et al.Applications of carbon nanomaterials in the plant system:A perspective view on the pros and cons[J].The Science of the Total Environment,2019,667:485-499.[9]FLORES D,CHACN R,ALVARADO L,et al.Effect of using two different types of carbon nanotubes for blackberry (Rubus adenotrichos) in vitro plant rooting,growth and histology[J].American Journal of Plant Sciences,2014,5(24):3510-3518.[10]XIONG J L,LI J,WANG H C,et al.Fullerol improves seed germination,biomass accumulation,photosynthesis and antioxidant system in Brassica napus L.under water stress[J].Plant Physiology and Biochemistry:PPB,2018,129:130-140.[11]SELVARAJ M,HAI A,BANAT F,et al.Application and prospects of carbon nanostructured materials in water treatment:A review[J].Journal of Water Process Engineering,2020(33):100996.[12]GIRI A,TOMKO J,GASKINS J T,et al.Large tunability in the mechanical and thermal properties of carbon nanotube-fullerene hierarchical monoliths[J].Nanoscale,2018,10(47):22166-22172.[13]KONG D,ZHONG L,WU C,et al.QHOD-net:A new highly metallic two-dimensional carbon allotrope material[J].Transactions of the Indian Institute of Metals,2023,76(3):829-835.[14]LI P,FENG D,FENG Y,et al.Measuring thermal conductivity of an individual carbon nanotube using Raman spectroscopy[J].Journal of Thermal Science,2022,31(4):1016-1022.[15]de LA ROSA G,GARCA-CASTAEDA C,VZQUEZ-NUEZ E,et al.Physiological and biochemical response of plants to engineered NMs:implications on future design[J].Plant Physiology and Biochemistry:PPB,2017,110:226-235.[16]GUO D L,LIU H N,WANG Z G,et al.Sodium dehydroacetate treatment prolongs the shelf-life of ‘Kyoho’ grape by regulating oxidative stress and DNA methylation[J].Journal of Integrative Agriculture,2022,21(5):1525-1533.[17]DEMIRER G S,ZHANG H,MATOS J L,et al.High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants[J].Nature Nanotechnology,2019,14:456-464.[18]刘晓静,冯宝春,冯守千,等.‘国光’苹果及其红色芽变花青苷合成与相关酶活性的研究[J].园艺学报,2009,36(9):1249-1254.[19]LISTER C E,LANCASTER J E,WALKER J R L.Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars[J].Journal of the Science of Food and Agriculture,1996,71(3):313-320.[20]ZHAO J,TAN S,LI H,et al.Multi-walled carbon nanotubes remediate the phytotoxicity of quinclorac to tomato[J].Bulletin of Environmental Contamination and Toxicology,2022,109(3):477-483.[21]MATHEW S,TIWARI D K,TRIPATHI D.Interaction of carbon nanotubes with plant system:a review[J].Carbon Letters,2021,31(2):167-176.[22]刘宏印,何树海,殷莉,等.冀南地区“摩尔多瓦” 葡萄的种植表现及关键栽培技术[J].北方园艺,2023(24):154-157.[23]王璐伟,陈利娜,李好先,等.不同类型果袋对天使红石榴果实品质的影响[J].果树学报,2024,41(1),113-121.[24]代红军,魏强,贺琰,等.油菜素内酯对高温胁迫下葡萄花色苷合成及果实品质的影响[J].园艺学报,2023,50(8):1711-1722.[25]王鹏洋,曲姗姗.ABA对果粒品质的影响研究进展[J].现代农业科技,2019(7):198.[26]李芳菲,马文瑶,程大伟,等.植物生长调节物质对葡萄着色影响的研究进展[J].果树学报,2019,36(7):928-938.[27]LIN Y,TAYLOR S,LI H,et al.Advances toward bioapplications of carbon nanotubes[J].Journal of Materials Chemistry,2004,14(4):527-541.[28]FAN X,XU J,LAVOIE M,et al.Multiwall carbon nanotubes modulate paraquat toxicity in Arabidopsis thaliana[J].Environmental Pollution,2018,233:633-641.[29]王应军,李娜,罗潇宇,等.多壁碳纳米管对铜绿微囊藻生长及生理特征的影响[J].生态毒理学报,2018,13(6):316-325.[30]杨晓娜,张星和,黄琪珍,等.食品配料及添加剂对双水相法提取红树莓花色苷稳定性的影响[J].北方园艺,2023(4):85-94.[31]郭西智,陈锦永,顾红,等.葡萄果实着色影响因素及改进措施[J].中外葡萄与葡萄酒,2016(5):124-127.[32]LARRONDE F,KRISA S,DECENDIT A,et al.Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars[J].Plant Cell Reports,1998,17(12):946-950.[33]GANJAVI A S,ORAEI M,GOHARI G,et al.Glycine betaine functionalized graphene oxide as a new engineering nanoparticle lessens salt stress impacts in sweet basil (Ocimum basilicum L.)[J].Plant Physiology and Biochemistry:PPB,2021,162:14-26.[34]GOHARI G,SAFAI F,PANAHIRAD S,et al.Modified multiwall carbon nanotubes display either phytotoxic or growth promoting and stress protecting activity in Ocimum basilicum L.in a concentration-dependent manner[J].Chemosphere,2020,249:126171.[35]SAMANTA S,SINGH A,BANERJEE A,et al.Exogenous supplementation of melatonin alters representative organic acids and enzymes of respiratory cycle as well as sugar metabolism during arsenic stress in two contrasting indica rice cultivars[J].Journal of Biotechnology,2020,324:220-232.[36]SINGH M,AVTAR R,LAKRA N,et al.Early oxidative burst and anthocyanin-mediated antioxidant defense mechanism impart resistance against Sclerotinia sclerotiorum in Indian mustard[J].Physiological and Molecular Plant Pathology,2022,120:101847.[37]LI Y,LIU M,YANG X,et al.Multi-walled carbon nanotubes enhanced the antioxidative system and alleviated salt stress in grape seedlings[J].Scientia Horticulturae,2022,293:110698.[38]唐媛媛,牛腾飞,叶菊,等.异戊烯基黄酮的化学结构、药理活性及生物合成研究进展[J].分子植物育种,2023,21(10):3319-3329.[39]倪荣.蕨类植物查尔酮异构酶和查尔酮合酶功能研究[D].济南:山东大学,2019.[40]谢琦,何媛,王婉妮,等.外源糖对‘媚丽’葡萄酒发酵品质的影响[J].中外葡萄与葡萄酒,2023(5):94-102.[41]张梦燕,孙军利,赵宝龙,等.外源ALA对日光温室弗雷无核葡萄果实着色及相关酶活性的影响[J].华北农学报,2018,33(S1):139-145.[42]龙小凤.多壁碳纳米管诱导葡萄细胞白藜芦醇生物合成的研究[D].苏州:苏州大学,2013.[43]孔德馨,姜雅轩,张佩远,等.黑龙江省延寿县驯化软枣猕猴桃品种果实品质比较分析[J].北方园艺,2024(5):9-16.[44]于文剑,杨丽,张俊环,等.杏果实风味形成及调控机制研究进展[J].果树学报,2023,40(12):2624-2637.[45]朱盼盼,王录俊,李蕊,等.不同配比GA3和CPPU对葡萄果实品质的影响[J].安徽农业科学,2018,46(19):38-40,62.
相似文献/References:
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备注/Memo
第一作者简介:葸金山(1999-),男,硕士研究生,研究方向为果树栽培生理。E-mail:1417297875@qq.com.责任作者:于坤(1985-),男,博士,副教授,现主要从事果树栽培生理等研究工作。E-mail:yukun410@163.com.基金项目:国家自然科学基金资助项目(31760550);八师石河子市中青年科技创新人才计划资助项目(2022RC01);石河子大学高层次人才资助项目(RCZK201925);兵团 “青年强”科技创新骨干人才计划资助项目(2023CBO08-05);石河子大学科学研究资助项目(CXFZ202016)。收稿日期:2024-01-30