LU Panling,DU Xuan,WANG Ying,et al.Effects of Salt-alkaline Stress on Physiological Characteristics of Salt Tolerance in Chieh-qua Seedlings[J].Northern Horticulture,2024,(15):8-14.[doi:10.11937/bfyy.20240347]
碱性盐胁迫对节瓜幼苗耐盐生理特性的影响
- Title:
- Effects of Salt-alkaline Stress on Physiological Characteristics of Salt Tolerance in Chieh-qua Seedlings
- 文章编号:
- 1001-0009(2024)15-0008-07
- Keywords:
- chieh-qua; salt-alkaline stress; reactive oxygen species; osmotic regulation; chlorophyll fluorescence
- 分类号:
- S 642
- 文献标志码:
- A
- 摘要:
- 以‘菲绿达1号’节瓜为试材，采用水培的方法，设置不同的碱性盐NaHCO3（0、25、50、75、100 mmol·L-1和125 mmol·L-1）处理，研究了不同浓度碱性盐胁迫对节瓜生理特性的影响，以期为碱性盐胁迫对节瓜的胁迫损伤机理提供参考依据。结果表明：25、50 mmol·L-1等低浓度盐胁迫对节瓜幼苗的影响不显著，但较高浓度的盐胁迫使节瓜幼苗的光合系统出现损伤，Y(Ⅱ)、ETR、qP、Fv/Fm出现显著上升。此外，高浓度的盐胁迫使得节瓜的活性氧含量（O·2和H2O2）和多种抗氧化酶活性显著上调；盐胁迫还使丙二醛（MDA）含量和相对电导率显著上升，这可能是ROS过量积累引起的；研究还发现高浓度的盐胁迫还促进了脯氨酸、可溶性糖和可溶性蛋白质等渗透调节物质的显著上升。综上所述，25、50 mmol·L-1盐溶液对节瓜幼苗的损伤有限，但75 mmol·L-1及以上的盐浓度超出节瓜幼苗的耐受范围；此外，节瓜主要通过增加光合热耗散、提高抗氧化酶活性和增加渗透调节物的合成来应对盐胁迫。
- Abstract:
- Chieh-qua seedlings ‘Feicuida 1’ was selected as the experimental material，the method of hydroponics was adopted and different salt concentrations (0，25，50，75，100，125 mmol·L-1) were set up to observe and measure the physiological response of chieh-qua seedlings,in order to provide reference for the damage mechanism of alkaline salt stress on chieh-qua seedlings. The results showed that low concentrations of salt stress such as 25，50 mmol·L-1 had no obvious effect on the seedlings，but higher concentrations of salt stress caused damage to the photosynthetic system of the seedlings，and Y(Ⅱ)，ETR，qP，Fv/Fm increased significantly.In addition，the ROS content (O·〖TX--*9〗2 and H2O2) and the activities of various antioxidant enzymes were significantly up-regulated under high concentration salt stress.Salt stress also significantly increased MDA content and relative conductivity，which may be caused by excessive accumulation of ROS.Finally，the study also found that the high concentration of salt stress also promoted the significant increase of osmoregulatory substances such as Pro，soluble sugar and soluble protein.In conclusion，the damage of 25，50 mmol·L-1 salt solution was limited，but the salt concentration of 75 mmol·L-1 and above exceeded the tolerance range of chieh-qua seedlings.In addition，the chieh-qua could cope with salt stress by increasing photosynthetic heat dissipation，increasing antioxidant enzyme activity and increasing osmoregulator synthesis.
参考文献/References:
［1］吴嘉垲,杨晨,欧立军.盐胁迫对不同辣椒品种种子萌发特性的影响［J］.辣椒杂志,2023,21(3):20-23.［2］刘德兴,荆鑫,焦娟,等.嫁接对番茄产量、品质及耐盐性影响的综合评价［J］.园艺学报,2017,44(6):1094-1104.［3］刘娜,杜旋,鲁博,等.不同破壳率对节瓜种子发芽及幼苗生长的影响［J］.上海农业学报,2021,37(6):60-64.［4］何晓明,彭庆务,王敏,等.我国节瓜遗传育种研究进展［J］.广东农业科学,2021,48(9):1-11.［5］秦曼丽,胡绪峰,刘德麒,等.硅对盐胁迫下黄瓜生长和多胺代谢的影响［J］.植物生理学报,2022,58(6):1077-1091.［6］张子健,刘美岑,张东,等.盐胁迫对甜瓜幼苗根系生长及膜脂过氧化的影响［J］.内蒙古农业大学学报(自然科学版),2022,43(2):11-16,101.［7］王伟奇,张蒙,秦肇辰,等.南瓜耐盐性研究进展［J］.中国蔬菜,2020(10):18-26.［8］张瑜,吴才君,苏文桢,等.西瓜OSCA基因家族全基因组鉴定及胁迫响应分析［J］.南方农业学报,2021,52(12):3330-3339.［9］徐新娟,李勇超.2种植物相对电导率测定方法比较［J］.江苏农业科学,2014,42(7):311-312.［10］方怡然,薛立.盐胁迫对植物叶绿素荧光影响的研究进展［J］.生态科学,2019,38(3):225-234.［11］BARBER J.‘Photosystem Ⅱ:The water splitting enzyme of photosynthesis and the origin of oxygen in our atmosphere［J］.Quarterly Reviews of Biophysics,2016,49:e14.［12］ZABRET J,BOHN S,SCHULLER S K,et al.Structural insights into photosystem Ⅱ assembly［J］.Nature Plants,2021(7):524-538.［13］严蓓,孙锦,束胜,等.外源钙对NaCl胁迫下黄瓜幼苗叶片光合特性及碳水化合物代谢的影响［J］.南京农业大学学报,2014,37(1):31-36.［14］程玉静,郭世荣,束胜,等.外源硝酸钙对盐胁迫黄瓜幼苗生长和光合作用的影响［J］.江西农业学报,2012,24(4):38-41,45.［15］董杰,陈新新,杨倩,等.高光、水分和盐胁迫下小麦光合特性和抗氧化酶系统的比较［J］.麦类作物学报,2018,38(3):315-322.［16］徐晨,刘晓龙,李前,等.供氮水平对盐胁迫下水稻叶片光合及叶绿素荧光特性的影响［J］.植物学报,2018,53(2):185-195.［17］周晓瑾,黄海霞,张君霞,等.盐胁迫对裸果木幼苗光合特性的影响［J］.草业学报,2023,32(2):75-83.［18］XIE D L,ZHENG X L,ZHOU C Y,et al.Functions of redox signaling in pollen development and stress response［J］.Antioxidants,2022,11(2):287.［19］WANG W,LIU S,YAN M.Synthesis of γ-aminobutyric acid-modified chitooligosaccharide derivative and enhancing salt resistance of wheat seedlings［J］.Molecules,2022,27(10):3068.［20］杨林林,韩敏琦,高嘉,等.不同形态硒对盐胁迫下小麦苗期光合生理、抗氧化系统及离子稳态的影响［J］.山东农业科学,2023,55(9):46-56.［21］YASIR T A,KHAN A,SKALICKY M,et al.Exogenous sodium nitroprusside mitigates salt stress in lentil (Lens culinaris Medik.) by affecting the growth,yield,and biochemical properties［J］.Molecules,2021,26(9):2576.［22］GILL R A,ZANG L,ALI B,et al.Chromium-induced physio-chemical and ultrastructural changes in four cultivars of Brassica napus L［J］.Chemosphere,2015,120:154-164.［23］YU H,ZHAO W,WANG M,et al.The exogenous application of spermidine alleviates photosynthetic inhibition and membrane lipid peroxidation under low-light stress in tomato (Lycopersicon esculentum Mill.) seedlings［J］.Plant Growth Regulation,2016,78(3):413-420.［24］杨宏伟,刘文瑜,沈宝云,等.NaCl胁迫对藜麦种子萌发和幼苗生理特性的影响［J］.草业学报,2017,26(8):146-153.［25］贾庆美,胡克玲.外源H2O2对盐胁迫下甜瓜生理特性的影响［J］.安徽农学通报,2023,29(14):39-42,55.［26］胡高峰,张小勇,莫海涛,等.抗盐碱制剂对棉花幼苗生理生化特性的影响［J］.西北农业学报,2009,18(2):169-172.［27］岳新丽,湛润生,牛雅玉,等.NaCl胁迫对黄花菜种子萌发和幼苗生长的影响［J］.中国农学通报,2023,39(16):35-40.［28］高桐梅,吴寅,李丰,等.苗期水分胁迫对芝麻生长和生理特性的影响［J］.核农学报,2017,31(11):2229-2235.［29］熊超明,杨婉莹,王文晓,等.不同盐碱胁迫对草莓生长与生理的影响［J］.北方园艺，2023(11):38-44.
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(15):128.
[3]张 芬,张 波,田丽萍,等.盐胁迫对番茄幼苗叶片光合特性及叶绿素和β-胡萝卜素含量的影响[J].北方园艺,2014,38(11):15.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves[J].Northern Horticulture,2014,38(15):15.
[4]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(15):64.
[5]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(15):86.
[6]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(15):65.
[7]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(15):1.
[8]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(15):75.
[9]刘 杰.盐碱胁迫对向日葵体内矿质营养的影响[J].北方园艺,2014,38(02):1.
LIU Jie.Effects of Simulated Salt and Alkali Conditions on the Mineral Nutrition of Helianthus annuusL.[J].Northern Horticulture,2014,38(15):1.
[10]张芬,张波,田丽萍,等.盐胁迫对加工番茄叶片番茄红素[J].北方园艺,2014,38(04):82.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Expression of Lycopene Cyclase Gene in Processing Tomato Leaves[J].Northern Horticulture,2014,38(15):82.
备注/Memo
第一作者简介：卢盼玲（1993-），女，硕士，研究实习员，现主要从事蔬菜育种与栽培等研究工作。E-mail：lpl2245@163.com.责任作者：刘娜（1982-），女，博士，副研究员，现主要从事节瓜育种与栽培等研究工作。E-mail：yyliuna@163.com.基金项目：上海市科技兴农资助项目（沪农科推字［2022］第1-20号）。收稿日期：2024-01-23