LIN Rongyan,CHEN Yiquan,KONG Lan,et al.Identification and Genetic Diversity Analysis of Hybrid Progenies of Dendrobium Based on SRAP Markers[J].Northern Horticulture,2024,(15):46-52.[doi:10.11937/bfyy.20240074]
石斛兰杂交后代SRAP鉴定与遗传多样性分析
- Title:
- Identification and Genetic Diversity Analysis of Hybrid Progenies of Dendrobium Based on SRAP Markers
- 文章编号:
- 1001-0009(2024)15-0046-07
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 以麝香石斛、檀香石斛及麝香石斛×檀香石斛的100个杂交后代株系为试材，采用SRAP分子标记的方法，研究了杂交后代的真实性及亲本与杂交后代群体的遗传多样性，以期为加速石斛兰新品种的选育提供参考依据。结果表明：综合6对SRAP引物组合的扩增情况，共鉴定出100个杂交后代为真杂种，鉴定效率达100%；6对SRAP引物组合的多态性比例达97.26%，多态性信息含量均值为0.85；由聚类分析可知，92%的杂交后代在亲缘关系上与父本聚为一类；由遗传相似系数分析可知，杂交后代与父本间的遗传相似系数平均值大于母本。综上所述，供试的麝香石斛×檀香石斛杂交后代均为真杂种，且表现为偏父本遗传，SRAP分子标记可作为石斛兰杂交后代早期鉴定的有效手段。
- Abstract:
- One hundred hybrid progenies of Dendrobium progenies,D.anosmum and D.porishii×D.anosmum were used as the test materials，the authenticity of the hybrid progenies was identified by SRAP molecular markers，their genetic diversity was also analyzed，in order to provide reference for accelerating the breeding process of new Dendrobium varieties.The results showed that 100 hybrid progenies were identified as true hybrids，with an identification efficiency of 100%，based on the amplification of 6 pairs of SRAP primer combinations.The polymorphic ratio of the 6 pairs of SRAP primer combinations was 97.26%，and the average of polymorphism information content was 0.85.According to the cluster diagram，92% of the hybrid offspring were clustered with the paternal parent in terms of parentage.According to the box-plot of genetic similarity coefficient analysis，the average genetic similarity coefficient between the hybrid offspring and the male parent was greater than that of the female parent，which indicated that the offspring of the hybrid combination were genetically more biased towards the male parent.In conclusion，the tested hybrid offspring of D.parishii×D.anosmum were all true hybrids and showed partial paternal inheritance.SRAP molecular markers could be used as an effective means for early identification of hybrid offspring in Dendrobium.
参考文献/References:
［1］周胜芳，夏豫川，刘钰，等．石斛SSR分子标记的研究进展［J］．分子植物育种，2023，21(4)：1239-1254．［2］ZHENG S G，HU Y D，ZHAO R X，et al．Genome-wide researches and applications on Dendrobium［J］．Planta，2018，248(4)：769-784．［3］叶秀仙，陈艺荃，林榕燕，等．石斛兰杂交结实性研究［J］．福建农业科技，2021，52(3)：1-5．［4］李慧峰，冉昆，王涛．利用SRAP标记构建山东省苹果资源指纹图谱［J］．沈阳农业大学学报，2020，51(4)：470-475．［5］MOHAMMED N A，AFZAL M，AL-FAIFI S A，et al．Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes［J］．Plant Biotechnology Reports，2023，17(4)：519-530．［6］VINODHINI R T，ASHWIN N M R，AGISHA V N，et al．Discovering the molecular variations among distinct sporisoriumscitamineum isolates using sequence-related amplified polymorphism (SRAP) markers［J］．Sugar Tech，2023，25(5)：1144-1151．［7］LIU J，CAI G，LI X，et al．Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer′s yeast［J］．Molecular Biology Reports，2023，50(2)：1943-1948．［8］ZHAO X，ZOU G，ZHAO J，et al．Genetic relationships and diversity among populations of Paris polyphylla assessed using SCoT and SRAP markers［J］．Physiology and Molecular Biology of Plants：An International Journal of Functional Plant Biology，2020，26(6)：1281-1293．［9］田彦挺，李际红，王锦楠，等．窄冠型杨树杂交子代SRAP鉴定及其遗传变异研究［J］．核农学报，2018，32(5)：875-882．［10］蒋小刚，王华，张美德．白术种质资源遗传多样性的SRAP分析［J］．北方园艺，2022(1)：109-115．［11］BUDAK H，SHEARMAN R C，PARMAKSIZ I，et al．Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers［J］.Theoretical and Applied Genetics，2004，108(2)：328-334．［12］杨军，游小妹，陈常颂．利用荧光SSR标记鉴定茶树自然杂交后代遗传背景［J］．西北植物学报，2023，43(12)：1981-1993．［13］WANG F Q，BIAN L，QIU P P，et al．Development and application of KASP marker for high throughput detection of the seedless trait in grapevine［J］．Journal of Integrative Agriculture，2023，22(11)：3269-3283．［14］PATEL V，TRIPATHI M K，SHRIVASTAVA R K，et al．Detection of true hybrids in pearl millet cross combinations by employing SSR molecular markers［J］．International Journal of Environment and Climate Change，2023，13(10)：2366-2377．［15］孙晓梅，崔金秋，王慧聪，等．芍药属植物杂交亲和性及杂交后代ISSR分子鉴定［J］．北方园艺，2016(9)：98-101．［16］刘玉洋，徐靖，何金宇，等．铁皮石斛和金钗石斛杂交后代的分子鉴定［J］．浙江农业科学，2018，59(9)：1709-1713．［17］杨柳燕，杨贞，蔡友铭，等．SRAP分子标记在铁皮石斛后代品种鉴定中的应用［J］．分子植物育种，2018，16(17)：5690-5695．［18］林榕燕，陈艺荃，钟淮钦，等．SRAP标记在秋石斛杂交后代鉴定中的应用［J］．江苏农业科学，2021，49(22)：55-59．［19］林榕燕，罗远华，樊荣辉，等．文心兰杂交后代的EST-SSR和SRAP分子标记鉴定［J］．福建农业学报，2021，36(12)：1439-1446．［20］刘颖鑫，李沛曈，迟天华，等．菊花脑×甘菊种间F1杂种的鉴定和遗传多样性分析［J］．园艺学报，2019，46(8)：1553-1564．［21］文雁成，张书芬，何俊平，等．基于基因芯片的甘蓝型油菜优异材料杂种优势群划分［J］．分子植物育种，2023，21(11)：3785-3797．［22］赵秋菊，崔学强，邓杰玲，等．基于iPBS标记的石斛兰杂交子代真实性鉴定［J］．热带作物学报，2023，44(6)：1153-1160．
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备注/Memo
第一作者简介：林榕燕（1990-），女，硕士，助理研究员，现主要从事花卉生物技术等研究工作。E-mail:lryyan@163.com.责任作者：叶秀仙（1977-），女，本科，副研究员，现主要从事花卉育种与组织培养技术等研究工作。E-mail:yxx7861@163.com.基金项目：福建省省属公益类科研院所资助项目 （2022R1031009）；福建省农业科学院对外合作资助项目（DWHZ-2023-06）；福建省农业科学院自由探索科技创新资助项目（ZYTS2023003）；福建省种业工程资助项目（ZYCX-LY-202102）；福建省农业科学院科技创新团队建设资助项目（CXTD2021010-2）。收稿日期：2024-01-05