LYU Qiyu,ZHANG Xiuting,GONG Yuzhu,et al.Study on Screening of Anti-Trichoderma harzianum Strains of Oudemansiella raphanipes[J].Northern Horticulture,2024,(15):118-127.[doi:10.11937/bfyy.20234537]
卵孢小奥德蘑抗哈茨木霉菌株的筛选研究
- Title:
- Study on Screening of Anti-Trichoderma harzianum Strains of Oudemansiella raphanipes
- 文章编号:
- 1001-0009(2024)15-0118-10
- 分类号:
- S 603.4
- 文献标志码:
- A
- 摘要:
- 以全国收集的26株卵孢小奥德蘑菌株为试材，采用人工抗逆境驯化和原生质体育种方法，研究了卵孢小奥德蘑菌株在逆境下的生长特性，以期为卵孢小奥德蘑优良菌株选育提供参考依据。结果表明：对采集的26株卵孢小奥德蘑菌株，经过（26 ℃，16 h；30 ℃，8 h）、（26 ℃，16 h；32 ℃，8 h）、（26 ℃，16 h；34 ℃，8 h）、（26 ℃，16 h；36 ℃，8 h）条件下进行耐高温菌株驯化，筛选出Sgw、H08gw、Lgw、Qgw、H09gw、H01gw等6株耐高温菌株；通过卵孢小奥德蘑菌株与哈茨木霉菌株的对峙试验，筛选出对木霉抗性较强的F2、Sgw、Lgw 3株菌株；以耐高温驯化菌株（Sgw、H08gw）、抗木霉菌株（F2、Sgw、Lgw）为母本进行原生质体融合，并在（26 ℃，16 h；32 ℃，8 h）条件下再生，筛选出4株抗逆性较强菌株Y1、Y4、Z1、Z9；经出菇验证，菌株Y4、Y1、Sgw耐高温，产量高，抗木霉污染。经对抗逆性菌株酶活性指标测定、抗性菌株转录组分析，发现抗木霉菌株菌丝细胞代谢能力，催化活性，细胞结构体等功能发生了变化，并通过信号转导、碳水化合物代谢、氨基酸代谢、过氧化物酶体等代谢通路，以达到抵御哈茨木霉侵染和高温胁迫的作用。Y4、Y1、Sgw菌株为该研究筛选的最终优良菌株，可作为生产上进一步优化培育。
- Abstract:
- Taking 26 Oudemansiella raphanipes strains collected from the whole country as test materials，the breeding of Oudemansiella raphanipes strains with stress resistance was studied by means of artificial stress resistance domestication and protoplast sports breeding，in order to provide reference for the breeding of Oudemansiella raphanipes excellent strains.The results showed that 26 strains of Oudemansiella raphanipes were collected，after (26 ℃，16 hours；30 ℃，8 hours)，(26 ℃，16 hours；32 ℃，8 hours)，(26 ℃，16 hours；34 ℃，8 hours)，(26 ℃，16 hours;36 ℃，8 hours) under the condition of six high temperature resistant strains，Sgw，H08 gw，Lgw，Qgw，H09 gw and H01 gw，were screened out.Through the confrontation test between Oudemansiella raphanipes and Trichoderma harzianum strains，three strains of F2，Sgw and Lgw with strong resistance to Trichoderma harzianum were screened.Protoplast fusion was carried out with high temperature resistant domesticated strains (Sgw，H08gw) and Trichoderma harzianum resistant strains (F2，Sgw，Lgw) as female parents，and protoplast fusion was carried out at (26 ℃，16 hours；32 ℃，8 hours)，and 4 strains with strong stress resistance were screened out,Y1，Y4，Z1 and Z9.Y4，Y1 and Sgw had high temperature resistance，high yield and resistance to Trichoderma harzianumpollution.Through the determination of the enzyme activity index of the stress-resistant strain and the transcriptome analysis of the resistant strain，it was found that the metabolic ability，catalytic activity，cell structure and other functions of the mycelium cells of the Trichoderma harzianum-resistant strain changed，and through signal transduction，carbohydrate metabolism，amino acid metabolism，peroxisome and other metabolic pathways，in order to achieve the effect of resisting Trichoderma harzianum infection and high temperature stress.Y4，Y1 and Sgw strains were the final excellent strains screened in this study，which could be further optimized and cultivated in production.
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备注/Memo
第一作者简介：吕骐羽（1998-），男，硕士，助理农艺师，现主要从事食用菌品种选育等研究工作。E-mail:lvqiyu88@163.com.责任作者：姜淑霞（1962-），女，硕士，教授，现主要从事食用菌栽培等研究工作。E-mail：jsx6206@163.com.基金项目：中国华能集团有限公司总部科技资助项目（HNKJ21-HF293）。收稿日期：2023-12-27