[1]董然,金鸽,林强,等.湖北百合LhrMYB1、LhrMYB12、LhrMYB15及LhrMYB16基因克隆及生物信息学分析[J].北方园艺,2024,(12):42-51.[doi:10.11937/bfyy.20234392]
　DONG Ran,JIN Ge,LIN Qiang,et al.Cloning and Bioinformatics Analysis of LhrMYB1,LhrMYB12,LhrMYB15 and LhrMYB16 Genes in Lilium henryi Baker[J].Northern Horticulture,2024,(12):42-51.[doi:10.11937/bfyy.20234392]
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湖北百合LhrMYB1、LhrMYB12、LhrMYB15及LhrMYB16基因克隆及生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年12 页码: 42-51 栏目: 出版日期: 2024-06-30
Title:
Cloning and Bioinformatics Analysis of LhrMYB1,LhrMYB12,LhrMYB15 and LhrMYB16 Genes in Lilium henryi Baker
文章编号:
1001-0009(2024)12-0042-10
作者:
董然1; 金鸽2; 3; 林强2; 3; 韩东洋2; 3; 钟慧烨3; 4; 张秀海3
（1.梧州学院 六堡茶现代产业学院，广西 梧州 543002；2.吉林农业大学 林学与草学学院，吉林 长春130118；3.北京市农林科学院，北京 100097；4.西藏农牧学院 资源与环境学院，西藏 林芝 860000）
Author(s):
DONG Ran1; JIN Ge2; 3; LIN Qiang2; 3; HAN Dongyang2; 3; ZHONG Huiye3; 4; ZHANG Xiuhai3
(1.Liupao Tea Modern Industry College，Wuzhou University，Wuzhou，Guangxi 543002；2.College of Forestry and Grassland Science，Jilin Agricultural University，Changchun,Jilin 130118；3.Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097；4.College of Resources and Environment，Xizang Agriculture and Animal Husbandry College，Nyingchi,Xizang 860000)
关键词:
湖北百合; MYB; 基因克隆; 生物信息学分析
Keywords:
Lilium henryi Baker; MYB; gene cloning; bioinformatics analysis
分类号:
S 682.29
DOI:
10.11937/bfyy.20234392
文献标志码:
A
摘要:
以湖北百合为试材，采用RT-PCR方法克隆MYB基因，利用DNAMAN 8.0软件、ExPaSy-ProParam软件、NetPhos 3.1 Server等工具，研究了MYB基因所编码蛋白的结构以及功能，以期为开展湖北百合相关MYB转录因子的研究提供参考依据。结果表明：LhrMYB1、LhrMYB15蛋白为稳定蛋白、碱性蛋白、亲水性蛋白，LhrMYB12蛋白为稳定蛋白、酸性蛋白、亲水性蛋白；LhrMYB16蛋白为不稳定蛋白、碱性蛋白、亲水性蛋白。LhrMYB1与芒果中MYB3-like亲缘关系最近（XM 044620835），LhrMYB12与百合杂交品种亲缘关系最近（MK182389），LhrMYB15与百合杂交品种 division I中MYB15like亲缘关系最近（LC218141），LhrMYB16与百合杂交品种 division I中MYB16-1亲缘关系最近（LC218139）。
Abstract:
Taking Lilium henryi Baker as the test material，the MYB gene was cloned using RT-PCR method.The structure and function of the protein encoded by the MYB gene were studied using tools such as DNAMAN 8.0，ExPaSy-ProParam，and NetPhos 3.1 Server，in order to provide reference for the study of MYB transcription factors relused to Lilium henryi Baker.The results showed that LhrMYB1 and LhrMYB15 proteins were stable proteins，alkaline proteins，and hydrophilic proteins，while LhrMYB12 protein was stable protein，acidic protein，and hydrophilic protein；LhrMYB16 protein was an unstable protein，alkaline protein，and hydrophilic protein.LhrMYB1 had the closest relationship with MYB3-like in mango (XM 044620835)，LhrMYB12 had the closest relationship with lily hybrid variety (MK182389), LhrMYB15 had the closest relationship with lily hybrid variety division I MYB15like (LC218141)，and LhrMYB16 had the closest relationship with lily hybrid variety division I MYB16-1 (LC218139).

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备注/Memo

第一作者简介：董然（1966-），女，博士，教授，现主要从事经济植物资源与利用等研究工作。E-mail:1836630983@qq.com.责任作者：张秀海（1972-），男，博士，研究员，现主要从事百合分子育种等研究工作。E-mail:zhangxiuhai@baafs.net.cn.基金项目：北京市农林科学院创新能力专项资助项目（KJCX20230106）；现代农业产业技术体系北京市创新团队资助项目（BAIC09-2023）；北京市农林科学院科研创新平台建设资助项目（PT2023-2）。收稿日期：2023-12-15

更新日期/Last Update: 2024-07-11