GAO Lianlian,WEN Can,TU Jing,et al.Isolation,Identification and Determination of Liquid Fermentation Ability of a Wild Stropharia rugosoannulata From Annulata Isolate[J].Northern Horticulture,2024,(10):112-118.[doi:10.11937/bfyy.20234319]
一株野生大球盖菇的分离鉴定及液体发酵能力测定
- Title:
- Isolation,Identification and Determination of Liquid Fermentation Ability of a Wild Stropharia rugosoannulata From Annulata Isolate
- 文章编号:
- 1001-0009(2024)10-0112-07
- Keywords:
- Stropharia rugosoannulata; isolation and identification; liquid fermentation; biomass; enzyme activity
- 分类号:
- S 646.1+9
- 文献标志码:
- A
- 摘要:
- 以采自沅江市的野生大型真菌Str1为试材，通过形态学以及分子生物学鉴定，明确其分类学地位。采用单因素试验对菌丝固体培养条件进行研究，同时通过添加黄麻粉优化该菌株液体发酵能力，以期为大球盖菇（Stropharia rugosoannulata）的深入研究和黄麻副产品的回收利用提供参考依据。结果表明：Str1菌株与序列号为MF 919477.1的球盖菇科，大球盖菇（Stropharia rugosoannulata）聚为一支，结合形态学特征将Str1鉴定为大球盖菇。菌丝生长最适温度为25 ℃、pH为7、最适碳源为葡萄糖、最适氮源为酵母粉，在液体培养基中添加0.10%黄麻粉摇瓶发酵7 d，可显著提升大球盖菇菌丝体生物量且提升木聚糖酶的分泌，但对羧甲基纤维素酶的分泌影响不大。
- Abstract:
- Str1,a wild macrofungus collected from Yuanjiang City,was identified by morphology and molecular biology to clarify its taxonomic status.Single factor test was used to study the solid culture conditions of mycelia,and the liquid fermentation capacity of the strain was optimized by adding jute powder,in order to provide reference for the further study of Stropharia rugosoannulata,and also be conducive to the recycling of jute by-products.The results showed that Str1 strain and Stropharia rugosoannulata (Stropharia rugosoannulata),serial number MF 919 477.1,converged into one branch.Str1 was identified as macroglobularis by morphological characteristics.The optimal temperature for mycelial growth was 25 ℃,pH was 7,the optimal carbon source was glucose,and the optimal nitrogen source was yeast powder.Adding 0.10% jute powder to the liquid medium for shaking fermentation for 7 days could significantly increase the mycelial biomass and the secretion of xylanase,but had little effect on the secretion of carboxymethyl cellulase.
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备注/Memo
第一作者简介：高恋恋（1999-），女，硕士研究生，研究方向为食用菌等真菌类。E-mail:2982135051@qq.com.责任作者：魏宝阳（1974-），男，博士，副教授，现主要从事食用菌等大型真菌类研究与教学等工作。E-mail:13467543153@163.com.基金项目：科技特派员服务乡村振兴资助项目（2021NK4176）;2021年中科荣耀（苏州）生物科技有限公司横向资助项目。收稿日期：2023-12-11