LUO Fuxun,LIU Jun,LI Zhilin,et al.Study on the Culture of F1 Protocorms and Rhizomes of Cymbidium and Doumbalium[J].Northern Horticulture,2024,(8):34-43.[doi:10.11937/bfyy.20233696]
大花蕙兰+豆瓣兰F1代原球茎和根状茎培养研究
- Title:
- Study on the Culture of F1 Protocorms and Rhizomes of Cymbidium and Doumbalium
- 文章编号:
- 1001-0009(2024)08-0034-10
- 关键词:
- 大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代; 根状茎; 原球茎; 组织培养; 结构观察
- Keywords:
- Cymbidium hybridium ‘Dafeng’ × C.serratum var.Goeringii ‘Taijishengmei’ F1 generation; rhizome; protocorm; tissue culture; structure observation
- 分类号:
- S 682.3
- 文献标志码:
- A
- 摘要:
- 以大花蕙兰‘大凤’（Cymbidium hybridium ‘Dafeng’）与豆瓣兰‘太极圣梅’（C.serratum var.Goeringii ‘Taijishengmei’）F1代原球茎、根状茎为试材，采用在1/2MS培养基中添加不同植物激素的方法，研究了不同植物激素对大花蕙兰‘大凤’与豆瓣兰‘太极圣梅’F1代原球茎与根状茎增殖和分化的影响，以期为兰属植物高效繁育提供参考依据。结果表明：以1/2MS+香蕉80.0 g·L-1+碳粉0.5 g·L-1为基本培养基，加入TDZ 1.0 mg·L-1和NAA 0.2 mg·L-1最有利于根状茎增殖，增殖率达到343.33%；基本培养基加6-BA 2.0 mg·L-1和NAA 0.3 mg·L-1有利于根状茎分化，分化率达到83.33%。原球茎的增殖最佳配方为基本培养基加TDZ 1.0 mg·L-1和NAA 0.2 mg·L-1，增殖率达到160.00%；原球茎分化最佳配方为基本培养基加6-BA 2.0 mg·L-1和NAA 0.4 mg·L-1，分化率为76.00%。经解剖观察，原球茎和根状茎二者均存在不透明的维管束中心，且根状茎所含不透明的维管束中心比例大于原球茎；此外，F1代根状茎的增殖、芽分化率均高于原球茎。无论根状茎和原球茎，其内部结构均有根的特征，从内到外依次由表皮、皮层和维管组织构成。
- Abstract:
- The primary protocorm and rhizome of Cymbidium hybridium ‘Dafeng’ and C.serratum var.Goeringii ‘Taijishengmei’ F1 were used as experimental materials.The method of adding different plant hormones in 1/2MS medium was adopted.The effects of different plant hormones on the proliferation and differentiation of F1 protocorms and rhizomes of Cymbidium hybridium ‘Dafeng’ and C.serratum var.Goeringii ‘Taijishengmei’ were studied,in order to provide reference for efficient breeding of Cymbidium.The results showed that 1/2MS+banana 80.0 g·L-1+carbon powder 0.5 g·L-1 as the basic medium，TDZ 1.0 mg·L-1 and NAA 0.2 mg·L-1 were the most beneficial to rhizomes proliferation，and the proliferation rate reached 343.33%.The addition of 6-BA 2.0 mg·L-1 and NAA 0.3 mg·L-1 in basic medium was beneficial to the differentiation of rhizomes，and the differentiation rate reached 83.33%.The optimum formula for the growth of protocorm was as follows,basic medium plus TDZ 1.0 mg·L-1 and NAA 0.2 mg·L-1，the growth rate reached 160.00%.The optimum formula for protocorm differentiation was as follows,6-BA 2.0 mg·L-1 and NAA 0.4 mg·L-1 were added in basic medium，and the differentiation rate was 76.00%.According to the anatomical observation，both protocorm and rhizomes had opaque vascular bundle centers，and the proportion of opaque vascular bundle centers in rhizome was larger than that in protocorm.In addition，the proliferation and bud differentiation rates of F1 generation rhizomes were higher than those of the original bulbs.Both rhizomes and protocorms have the characteristics of roots in their internal structures，which are composed of epidermis，cortex and vascular tissue from inside to outside.
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备注/Memo
第一作者简介：罗富寻（1998-），女，硕士研究生，研究方向为观赏植物资源利用与创新。E-mail:1769818790@qq.com.责任作者：王玉英（1980-），女，博士，副教授，现主要从事观赏植物资源利用与创新等研究工作。E-mail:wyysxp@126.com.基金项目：云南省重大科技专项资助项目（202102AE090052）；云南省基础研究资助项目（202001AT070101）；云南省创新团队资助项目（202105AE160012）。收稿日期：2023-10-27