CHEN Bin,YI Xiaochang.Screening of High-yield Laccase Strains in Mixed Culture of LYL263 From Ganoderma lucidum[J].Northern Horticulture,2024,(8):114-118.[doi:10.11937/bfyy.20233512]
红芝LYL263混合培养高产漆酶菌株的筛选
- Title:
- Screening of High-yield Laccase Strains in Mixed Culture of LYL263 From Ganoderma lucidum
- 文章编号:
- 1001-0009(2024)08-0114-05
- 分类号:
- S 567.31
- 文献标志码:
- A
- 摘要:
- 以产漆酶的红芝LYL263菌株为试材，采用不同微生物混合培养的方法，研究了红芝LYL263与苏平平菇、白金金针菇、凤尾菇混合培养的产酶效果和产酶条件，以期能筛选出提高红芝LYL263产漆酶的相容菌株以及与之相适应的产酶条件。结果表明：红芝LYL263与其相容菌株-苏平平菇混合培养后漆酶活性达到1 346.4 U·mL-1，比红芝LYL263单独培养产酶提高35.7%；红芝LYL263与苏平平菇的接种比例为4:2时，其产酶量最高，最优接种时间是同时接入红芝LYL263和苏平平菇。
- Abstract:
- Taking laccase-producing LYL263 from Ganoderma lucidum as test material,the enzyme production effect and conditions of LYL263 with Flammulina velutipes,Pleutotus ostreatus and Pleurotus pulmonarius were studied by the mixed culture method of different microorganisms,in order to screen out compatible strains and adaptive conditions that could improve the laccase-producing capacity of LYL263.The results showed that laccase activity reached 1 346.4 U·mL-1 after mixed culture of LYL263 and its compatible strain,Pleutotus ostreatus,which was 35.7% higher than that of LYL263 cultured alone.When the inoculation ratio of LYL263 and Pleutotus ostreatus was 4∶2,the enzyme production was the highest.The optimal inoculation time was to add LYL263 and Pleutotus ostreatus at the same time.
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备注/Memo
第一作者简介：谌斌(1967-)，男，博士，研究员，现主要从事真菌资源的开发利用等研究工作。E-mail：binchen1967cn@126.com.基金项目：国家自然科学基金资助项目（41661077）；广西自然科学基金面上资助项目（2011GXNSFA018080）。收稿日期：2023-10-15