ZHAO Le,WANG Lei.Effects of Different AMF Inoculations on Root Activity and Growth Characteristics of Ophiopogon japonicus Seedlings[J].Northern Horticulture,2024,(9):41-46.[doi:10.11937/bfyy.20233511]
不同丛枝菌根真菌（AMF）接种量对麦冬幼苗根系活力及生长特性的影响
- Title:
- Effects of Different AMF Inoculations on Root Activity and Growth Characteristics of Ophiopogon japonicus Seedlings
- 文章编号:
- 1001-0009(2024)09-0041-06
- Keywords:
- Ophiopogon japonicus; Rhizophagus intraradices; mycorrhizal infection rate; root activity; physiological and biochemical indexes
- 分类号:
- S 688.4
- 文献标志码:
- A
- 摘要:
- 以麦冬为试材，采用拌种接种方法，以等体积混合的苗圃土、蛭石和珍珠岩为培养基质，研究不同接种量（10、20、30、40 g）丛枝菌根真菌根内根孢囊霉（RI）对麦冬幼苗的苗高、根长、菌根侵染率、根系活力、叶绿素含量、相对电导率、可溶性蛋白质含量的影响，以期为麦冬生长建植提供更为有效的栽培方式。结果表明：接种根内根孢囊霉能与麦冬形成共生关系，其中接种40 g根内根孢囊霉时麦冬的根系侵染率最高。与对照相比，随着接种量的增加，显著促进了麦冬的生长，40 g处理下麦冬幼苗的株高、根长、分蘖数、叶面积、叶绿素含量、相对电导率和可溶性蛋白质含量均显著增加；灰色关联度分析也表明，AMF侵染率与植物的株高、分蘖数和相对电导率的关联度最大。
- Abstract:
- Taking Ophiopogon japonicus as the test material,and the mixed nursery soil,vermiculite and perlite with equal volume were used as the culture matrix.The seedling height,root length,mycorrhizal infection rate,root activity,chlorophyll content,relative conductivity and soluble protein content of Ophiopogon japonicus seedlings under different inoculation amounts (10,20,30,40 g) of arbuscular mycorrhizal fungi (AMF) were studied,in order to provide a more effective cultivation method for the growth and establishment of Ophiopogon japonicus.The results showed that Rhizophagus intraradices could form a symbiotic relationship with Ophiopogon japonicus,and Rhizophagus intraradices inoculated with 40 g had the highest root infection rate.Compared with the control,the growth of Ophiopogon japonicum seedlings was significantly promoted with the increase of inoculation amount,and the plant height,root length,tillering number,leaf area,chlorophyll content,relative electrical conductivity and soluble protein content of Ophiopogon japonicum seedlings under 40 g treatment were significantly increased.Grey correlation analysis also showed that AMF infection rate was most correlated with plant height,tiller number and relative electrical conductivity.
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备注/Memo
第一作者简介：赵乐（1998-），女，硕士研究生，研究方向为风景园林与人居环境。E-mail:1922639976@qq.com.责任作者：王雷（1972-），男，博士，副教授，现主要从事草坪生态学与园林植物等研究工作。E-mail:14747405563@163.com.基金项目：内蒙古自治区应用技术研究与开发资金资助项目（2021GG0075）;乌海市全域矿山排土场植被生态恢复战略规划资助项目（WHZCGF230038）。收稿日期：2023-10-15