[1]张新超,张新宇,张雪梅,等.‘绿岭’核桃带芽茎段的组织培养和快速繁殖[J].北方园艺,2023,(24):38-44.[doi:10.11937/bfyy.20232999]
　ZHANG Xinchao,ZHANG Xinyu,ZHANG Xuemei,et al.Tissue Culture and Rapid Propagation of ‘Lyuling’ Walnut Stems With Axillary Buds[J].Northern Horticulture,2023,(24):38-44.[doi:10.11937/bfyy.20232999]
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‘绿岭’核桃带芽茎段的组织培养和快速繁殖

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年24 页码: 38-44 栏目: 出版日期: 2023-12-30
Title:
Tissue Culture and Rapid Propagation of ‘Lyuling’ Walnut Stems With Axillary Buds
文章编号:
1001-0009(2023)24-0038-0
作者:
张新超1; 张新宇2; 张雪梅3; 4; 5; 李寒3; 4; 5; 齐国辉3; 4; 5
（1.河南省林业资监测院，河南 郑州 450045；2.东北林业大学 野生动物与自然保护地学院，黑龙江 哈尔滨 150040；3.河北农业大学 林学院，河北 保定 071000；4.河北省核桃工程技术研究中心，河北 临城 054300；5.河北省(邢台）核桃产业技术研究院，河北 临城 054300）
Author(s):
ZHANG Xinchao1; ZHANG Xinyu2; ZHANG Xuemei3; 4; 5; LI Han3; 4; 5; QI Guohui3; 4; 5
(1.Henan Resources Monitoring Institute，Zhengzhou，Henan 450045；2.College of Wildlife and Protected Area，Northeast Forestry University，Harbin,Heilongjiang 150040;3.Forestry College,Hebei Agricultural University，Baoding，Hebei 071000;4.Research Center for Walnut Engineering and Technology of Hebei，Lincheng，Hebei 054300;5.Institute of Walnut Industry Technology of Hebei Province (Xingtai)，Lincheng，Hebei 054300)
关键词:
核桃; 茎段; 组织培养; 快速繁殖
Keywords:
walnut; stem; tissue culture; rapid propagation
分类号:
S 664.1
DOI:
10.11937/bfyy.20232999
文献标志码:
A
摘要:
以‘绿岭’核桃带芽茎段为试材，采用组织培养方法，研究了植物生长调节剂、珍珠岩对其快速繁殖、瓶内生根及移栽成活的影响，以期构建‘绿岭’核桃初代培养、继代增殖、瓶内生根和移栽驯化的完整体系，建立高效组培快繁体系。结果表明：茎段腋芽生长的最佳培养基为DKW+1.2 mg·L-1 6-BA+0.012 mg·L-1 K-IBA，培养30 d后初代苗高为31.48 mm；最佳继代增殖培养基为DKW+0.6 mg·L-1 6-BA+0.010 mg·L-1 K-IBA，增殖系数可达 5.31；最佳瓶内生根培养基为1/2DKW+15 mg·L-1 K-IBA，生根率51.85%；炼苗后移栽到培养土、珍珠岩（1∶1）+ABT 500 mg·L-1混合基质中，成活率达 33%以上。
Abstract:
Taking the stem withbud of ‘Lyuling’ walnut as the test materials，the tissue culture method was used，the effects of plant growth regulators and perlite on rapid propagationon and in vitro rooting were studied,in order to set up the fittest medium and subculture for germination of stem axillary bud and the best rooting medium and the transplantation to built the tissue culture and rapid propagation. The results showed that the fittest medium for germination of stem axillary bud was DKW+1.2 mg·L-1 6-BA+0.012 mg·L-1 K-IBA，with a 31.48 mm germination high of axillary bud after culture for 30 days；the best medium for shoot multiplication was DKW+0.6 mg·L-1 6-BA+0.010 mg·L-1 K-IBA，and the multiplication coefficient could reach 5.31；the best rooting medium was 1/2DKW+15 mg·L-1 K-IBA perlite，with a 51.85% rooting rate.After the culture-bottle seedlings were transplanted into the matrix composed of peat soil and perlite (1∶1)+ABT 500 mg·L-1，the survival rate was up to 33%.

参考文献/References:

［1］李保国,齐国辉.绿色优质薄皮核桃生产［M］.北京:中国林业出版社,2007.［2］中国林业和草原年鉴［M］.北京:中国林业出版社，2021.［3］孟佳,方晓璞,史宣明,等.我国核桃产业发展状态、问题与建议［J］.中国油脂,2023,48(1):84-86.［4］刘喜星,顾玉红,李保国,等.‘绿岭’核桃种胚无叶腋芽萌发快繁途径的初步研究［J］.林业实用技术,2012（11):64-66.［5］刘丽丽,任涛.核桃嫁接繁殖苗木技术［J］.中国林副特产,2022（6):55-56.［6］TAYLOR M D，RAUP A.The effect of auxin and cutting type on rooting of cupressus cashmeriana［C］.Pennsylvania：Conference of the International Plant Propagators Society，2013.［7］ALIREZA F，SAHRA N，AFSANEH A，et al.Biochemical changes in terminal buds of three different walnut (Juglans regia L.) genotypes during dormancy break［J］.Biochemical Systematics and Ecology，2018,76(12)：52-57.［8］DONG N G，LI Y F，QI J X，et al.Nitric oxide synthase-dependent nitric oxide production enhances chilling tolerance of walnut shoots in vitro via involvement chlorophyll fluorescence and other physiological parameter levels［J］.Scientia Horticulturae,2018,230(11):68-77.［9］WIERZCHOWSKI K，CHEC〖JX-+0.8mm〗〖KG-*2〗＇KO J，PYKA I.Variability of mercury content in coal matter from coal seams of the upper Silesia coal basin［J］.Archives of Mining Sciences,2017,62(4):843-856.［10］张新超,张新宇,唐敏,等.植物生长调节剂对‘绿岭’核桃组培苗瓶内生根及内源激素含量的影响［J］.河北农业大学学报,2021,44(1):51-56,66.［11］张新超,张新宇,董一,等.消毒剂对‘绿岭’核桃茎段组织培养的影响［J］.林业与生态科学,2019,34(2):158-166.［12］李强.软枣猕猴桃外植体消毒及继代增殖研究［J］.新农业,2018(11):7-9.［13］胡文斌,张少飞,孙娜,等.核桃茎段组织培养无菌芽苗的诱导［J］.甘肃农业科技,2018(5):36-39.［14］黄靖,袁亚芳,刘春英.不同基质对铁皮石斛组培苗移栽成活率的影响［J］.福建农业科技,2018(10)：4-6.［15］刘笛,王悦,崔弘,等.吲哚丁酸对关苍术种子萌发及幼苗生长的影响［J］.种子,2018,37(7):84-86.［16］许丁帆,刘艳军,王赵囡,等.绒毛白蜡组培快繁体系的建立［J］.北方园艺,2021(19):78-83.［17］郭芳.‘海尔特兹’红树莓组织培养育苗技术及部分生理特性研究［D］.保定:河北农业大学,2017.［18］覃换玲,高营营,黄丽辉,等.小果甜柿组织培养快繁技术［J］.果树学报，2023,40(9):1992-2000.［19］陈凌艳,郑宇,陈礼光,等.西洋杜鹃组培苗生根培养及其内源激素含量变化的研究［J］.福建林学院学报,2011,31(2):131-135.［20］张文学,印迈,续晨,等.美国山核桃组织培养研究［J］.安徽农学通报,2019,25(11):22,43.［21］何崇单,姚锐,黄瑞春,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响［J］.中南林业科技大学学报,2017,37(10):7-12.［22］LI Z，ZHANG Y X，GAO Y.Atipamezole is a promising non-discriminative inhibitor against pan-CYP450 including diclofenac 4′-hydroxylation：A comparison with ABT for drug ADME optimization and mechanism study［J］.European Journal of Pharmaceutical Sciences,2019,130(1):156-165.［23］郭逸榴.石斛的组织培养技术及移栽探析［J］.种子科技,2023,41(5):1-3.［24］阿拉坦图雅,张丽,王园琳,等.苹果矮化砧组织培养研究进展［J］.中国南方果树,2023,29(9):21-23,37.［25］莫小锋,秦丽萍,文清岚,等.ABT生根粉、IBA和H2O2复合处理对台湾果桑扦插生根的影响［J］.中国南方果树,2018,47(3):102-106.

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备注/Memo

第一作者简介：张新超（1992-），女，硕士，助理工程师，现主要从事林业资源监测等研究工作。E-mail:1026349368@qq.com.责任作者：齐国辉（1968-），女，博士，教授，现主要从事经济林栽培生理等研究工作。E-mail:bdqgh@sina.com.基金项目：河北省重点研发计划资助项目（21326304D-2-1）。收稿日期：2023-08-30

更新日期/Last Update: 2024-01-02